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High-resolution Chromosomal Stripe Analysis Solution Specially Prepared for Karyotype Analysis

A technology of karyotype analysis and chromosome, which is applied in the biological field, can solve problems such as fuzzy banding analysis, and achieve the effect of improving solubility, obvious effect, fine and clear banding

Active Publication Date: 2021-10-15
BEIJING INST OF HUMAN GENETICS & REPRODUCTION MEDICINE LTD CHINA +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For the trypsin solution commonly used in the existing G-banding, there is a shortcoming of blurred band resolution, such as the microscopic photos of the G-banding disclosed in CN103308361 B and CN 103710435 B patents, see figure 1 , figure 2

Method used

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  • High-resolution Chromosomal Stripe Analysis Solution Specially Prepared for Karyotype Analysis
  • High-resolution Chromosomal Stripe Analysis Solution Specially Prepared for Karyotype Analysis
  • High-resolution Chromosomal Stripe Analysis Solution Specially Prepared for Karyotype Analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Prepare a high-resolution chromosome band analysis solution specially prepared for karyotype analysis, and each component of the band analysis solution includes:

[0046] Disodium hydrogen phosphate dodecahydrate 9-11g / L, potassium dihydrogen phosphate 1-2g / L, solvent 1L triple distilled water.

[0047] Wherein, it is prepared from the following raw materials:

[0048] A. Raw materials for weighing ratio: 10.14g of disodium hydrogen phosphate dodecahydrate, 1.33g of potassium dihydrogen phosphate;

[0049] B. Dissolve the above raw materials into 800ml three-distilled water, fully dissolve, adjust the pH to 7.25, and then set the volume to 1000ml;

[0050] C. Store at 4°C.

[0051] The high-resolution chromosome G-banding staining method comprises the following steps:

[0052]Step 1: In tank No. 1, pour 50ml of striped analysis solution, add 1ml of thawed trypsin, mix well, and preheat to 37°C;

[0053] in:

[0054] The working concentration of trypsin in experimen...

Embodiment 2

[0071] Prepare a high-resolution chromosome band analysis solution specially prepared for karyotype analysis, and each component of the band analysis solution includes:

[0072] Disodium hydrogen phosphate dodecahydrate 9-11g / L, potassium dihydrogen phosphate 1-2g / L, triple distilled water 1L.

[0073] Wherein, it is prepared from the following raw materials:

[0074] A. Raw materials for weighing ratio: 10.14g of disodium hydrogen phosphate dodecahydrate, 1.33g of potassium dihydrogen phosphate;

[0075] B. Dissolve the above raw materials into 800ml three-distilled water, fully dissolve, adjust the pH value, and then set the volume to 1000ml;

[0076] C. Store at 4°C.

[0077] in:

[0078] The pH value of the analytical solution in experimental group 7 was 7.2.

[0079] The pH value of the solution in the experimental group 8 was 7.3.

[0080] The pH value of the solution in the experimental group 9 was 7.4.

[0081] The pH value of the analytical solution of experimen...

Embodiment 3

[0096] Prepare a high-resolution chromosome band analysis solution specially prepared for karyotype analysis, and each component of the band analysis solution includes:

[0097] Disodium hydrogen phosphate dodecahydrate 9-11g / L, potassium dihydrogen phosphate 1-2g / L, triple distilled water 1L.

[0098] Wherein, it is prepared from the following raw materials:

[0099] A. Raw materials for weighing ratio: 10.14g of disodium hydrogen phosphate dodecahydrate, 1.33g of potassium dihydrogen phosphate;

[0100] B. Dissolve the above raw materials into 800ml three-distilled water, fully dissolve, adjust the pH to 7.4, and then set the volume to 1000ml;

[0101] C. Store at 4°C.

[0102] The high-resolution chromosome G-banding staining method comprises the following steps:

[0103] Step 1: In tank No. 1, pour 50ml of striped analysis solution, add 1ml of thawed trypsin, the working concentration of trypsin is 0.15g / L, mix well, and preheat to 37°C;

[0104] Step 2, No. 2 tank, ad...

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Abstract

The invention relates to a high-resolution chromosome banding analysis solution specially prepared for karyotype analysis and a preparation method thereof, and a high-resolution chromosome G banding and staining method using the high-resolution chromosome banding analysis solution specially prepared for karyotype analysis. High-resolution chromosome band analysis solution is specially prepared for karyotype analysis, which consists of the following components: disodium hydrogen phosphate dodecahydrate 9-11g / L, potassium dihydrogen phosphate 1-2g / L, NaF 0.01-0.04g / L, Phenylethyl ether 1‑5ml / L, the solvent is triple distilled water, and the pH value of the solution is 7.2‑7.6. The high-resolution chromosome band analysis solution is specially prepared for karyotype analysis in the present invention, which improves the solubility and dispersibility of trypsin granules. Capable of controlling and standardizing the digestive activity of trypsin and the uniformity of specimen digestion. The analysis effect of high-resolution chromosome banding is obvious, and the banding is fine and clear.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-resolution chromosome band analysis solution specially prepared for karyotype analysis. Background technique [0002] Chromosomes are the carriers of genes. Chromosomes may undergo quantitative and structural changes, which may cause various genetic defects, congenital genetic diseases, embryo cessation, spontaneous abortion, tumors, etc. Diseases caused by chromosomal abnormalities are called chromosomal disorders. Chromosomal detection is currently mostly used clinically for prenatal and postnatal care, reproduction, obstetrics, newborns, stem cell therapy, blood diseases, tumor inspection and prevention, etc. [0003] Chromosomal karyotype analysis is mainly divided into several steps: cell culture, harvest, preparation, banding and analysis. Among them, banding refers to the technique of using special enzyme treatment and then dyeing to show a fixed pattern of bright and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 李存玺刘恒涛刘舒李能干
Owner BEIJING INST OF HUMAN GENETICS & REPRODUCTION MEDICINE LTD CHINA