Nanometer silver/berberine slow release coating loaded calcium phosphate ceramic material and preparation method

A technology of calcium phosphate ceramics and berberine, applied in coatings, pharmaceutical formulations, pharmaceutical sciences, etc., can solve problems such as strong cytotoxicity, inability to be antibacterial with high-concentration nano-silver, and achieve the effect of reducing cytotoxicity

Inactive Publication Date: 2019-04-02
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: how to solve the problem that the existing calcium phosphate-based bioactive ceramic material loaded with nano-silver particles has strong cytotoxicity, which makes it impossible to use high-concentration nano-silver for antibacterial

Method used

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  • Nanometer silver/berberine slow release coating loaded calcium phosphate ceramic material and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Using silk fibroin to reduce silver nitrate in situ to prepare nano-silver, and incorporate berberine molecules to further prepare nano-silver / berberine slow-release coating. The calcium phosphate foaming material uses tricalcium phosphate powder as a raw material, and adopts a sintering molding method to prepare calcium phosphate ceramics. The nano-silver / berberine slow-release coating is connected to the surface of the calcium phosphate ceramic material through polydopamine.

[0028] (1) preparing a silk fibroin aqueous solution whose mass concentration is 5%;

[0029] (2) add silver nitrate and berberine to step (1) gained solution under stirring, the configuration silver nitrate concentration is 1mmol / L, the mixed liquor that berberine concentration is 50mg / L, concussion is even;

[0030] (3) UV light is carried out to the mixed solution obtained in step (2), and the light exposure time is 30min to obtain a uniform nano-silver / berberine / silk fibroin mixed solution;...

Embodiment 2

[0036] The product preparation process and steps are the same as in Example 1, except that the concentration of each functional drug in step (2) is adjusted, silver nitrate and berberine are added to the silk fibroin aqueous solution with a mass concentration of 5%, and the concentration of silver nitrate is configured. 2mmol / L, berberine concentration is the mixed solution of 50mg / L, shakes evenly, then according to the method in step (3) carries out ultraviolet light to the mixed solution, then soaks, is coated in the ceramic material of calcium phosphate substrate, obtains A bone repair material with good osteogenic activity and active antibacterial dual effects. This example further increases the concentration of silver ions in the functional coating, which shows better anti-staphylococcus aureus, Candida albicans, Escherichia coli antibacterial function.

Embodiment 3

[0038] The product preparation process and steps are the same as in Example 1, except that the concentration of each functional drug in step (2) is adjusted, silver nitrate and berberine are added to the silk fibroin aqueous solution with a mass concentration of 5%, and the concentration of silver nitrate is configured. 3mmol / L, berberine concentration is the mixed solution of 50mg / L, shakes evenly, then according to the method in step (3) carries out ultraviolet light to the mixed solution, then soaks, is coated in the ceramic material of calcium phosphate substrate, obtains A bone repair material with good osteogenic activity and active antibacterial dual effects. This example further increases the concentration of silver ions in the functional coating, which shows better anti-staphylococcus aureus, Candida albicans, Escherichia coli antibacterial function.

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Abstract

The invention discloses a nanometer silver / berberine slow release coating loaded calcium phosphate ceramic material and a preparation method. The nanometer silver / berberine slow release coating loadedcalcium phosphate ceramic material consists of calcium phosphate ceramics and nanometer silver / berberine composite slow release coating loaded on the surface of the calcium phosphate ceramics. The calcium phosphate ceramics are prepared through sintering calcium phosphate ceramic raw material powder, nanometer silver is prepared through in-situ reduction of silver nitrate through silk fibroin, berberine molecules are mixed, nanometer silver / berberine mixed liquor is obtained and is connected to the surface of the calcium phosphate ceramics through polydopamine, and the nanometer silver / berberine slow release coating loaded calcium phosphate ceramic material is prepared. According to the nanometer silver / berberine slow release coating loaded calcium phosphate ceramic material and the preparation method disclosed by the invention, berberine and antibacterial effects of nanometer silver granules are combined, so that the potential effects of the berberine for promoting osteogenic differentiation of cells can be sufficiently exerted. The berberine and the nanometer silver have the effect of cooperating for resisting bacteria, so that the concentration of the nanometer silver is reduced, and reduction of cytotoxicity of implantation materials is facilitated. The composite material can be applied to bone defect repair and oral cavity implants.

Description

technical field [0001] The invention belongs to the field of biological ceramic materials, in particular to a calcium phosphate ceramic material loaded with nano-silver / berberine slow-release coating and a preparation method. Background technique [0002] Calcium phosphate-based bioactive ceramic materials are similar to bone tissue in terms of inorganic structure and chemical composition. After implantation in the body, calcium and phosphorus ions on the surface of the material dissolve and deposit, so that new bone crystals are directly deposited on the surface of bone-like apatite. , can promote the proliferation and differentiation of bone cells, make bone tissue and implant materials form a tight and stable combination, and promote new bone formation. These excellent properties make it widely used clinically. However, this bioactive ceramic implant material also has shortcomings, and cannot inhibit the growth of bacteria, leading to infection and so on. [0003] To ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/50A61L27/54A61L27/30A61L27/28
CPCA61L27/12A61L27/28A61L27/306A61L27/50A61L27/54A61L2300/104A61L2300/216A61L2300/404A61L2300/45A61L2400/12A61L2430/02A61L2430/12
Inventor 孙建勋胡诚周长春宋平赵莉李磊吴丽娜高鹏孙桓范洪远
Owner SICHUAN UNIV
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