Type b Clostridium perfringens exotoxin and its preparation method, toxin-producing medium and application

Abstract

The invention discloses a type B Clostridium perfringens exotoxin, a preparation method thereof, a toxin-producing medium and an application thereof. Wherein every 1000ml described toxin-producing medium is made of the raw materials of following weight ratio: peptone 15-25g, yeast extract powder 3-5g, NaCl 3-4g, NaCl 2 HPO 4 12H 2 O 5‑10g, dextrin 5‑10g or crude dextrin 5‑10g, and the rest is water for injection. The virulence of the type B Clostridium perfringens toxin prepared by the method of the invention can reach up to 1 mouse MLD≤0.0006mL, which is 3.3 times higher than the seedling production standard. Moreover, the neutralization titer of the corresponding serum neutralization titers of rabbits and sheep in the triple inactivated vaccine of sheep rapid epidemic disease and sudden attack (or lamb dysentery) enterotoxemia also reaches or exceeds the standard of relevant regulations.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a type B Clostridium perfringens exotoxin, a preparation method thereof, a toxin-producing medium and applications thereof. Background technique [0002] Clostridium perfringens is a general term for diseases caused by pathogenic species of Clostridium. Clostridium is a class of anaerobic bacteria, there are more than 60 species, and only more than 10 common pathogenic bacteria, mostly present in soil, sewage, and human and various animal feces. Gram staining was positive, except for a few bacterial species, all had flagella, which could move and form spores, and their diameter was larger than that of bacteria. Most bacterial species can produce exotoxins with severe toxicity, which are not only the main factor of pathogenicity, but also the main antigen. After being transformed into toxoids, they can stimulate animals to produce antitoxins, which can be...

AI Technical Summary

Problems solved by technology

[0005] At present, the vaccines used in the industry generally use animal raw materials such as beef, fish, and beef liver to prepare anaerobic meat liver and stomach enzyme digestion soup and cod liver and stomach enzyme digestion soup medium, but using this method to prepare the medium is often due to raw materials. The quality is uneven, the difference between batches is large, and the phenomenon of unstable toxin production performance often occurs. Especially in recent years, due to the development of the domestic dairy industry, the number of dairy cows has increased rapidly, and a large number of eliminated dairy beef has flooded the market. The quality of the raw materials used to prepare the vaccine medium has declined; at the same time, the residual drugs in beef and beef liver often inhibit the growth of bacteria. decline in vaccine quality

In addition, the traditional medium preparation process is cumbersome, time-consuming, requires a lot of manpower, high price, and low recovery rate, which also brings great waste and high cost to vaccine production, causing great troubles to vaccine manufacturers

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