Veterinary clostidrium novyi exotoxin, as well as preparation method, toxin production culture medium and application

Abstract

The invention discloses a veterinary clostidrium novyi exotoxin, a preparation method thereof, a toxin production culture medium, and applications. Every 1000 ml of the toxin production culture mediumis prepared from the following raw materials in parts by weight: 15-25 g of peptone, 5-10 g of tryptone, 5-10 g of casein peptone, 1-3 g of yeast extract powder, 1-3 g of NaCl, 5-10 g of KH2PO4, 5-10g of dextrin or maltose, 95-105 g of rust-free iron nails or scrap iron, and the balance water for injection. The highest toxicity of a veterinary clostidrium novyi exotoxin solution prepared by themethod can reach 1MLD <= 0.00008 ml, and the toxicity is 6.25 times higher than that in a standard. In addition, the neutralizing titer of sheep black plague and bradsot combined inactivated vaccine prepared from the veterinary clostidrium novyi exotoxin in corresponding serum of rabbits and sheep also reaches or is higher than the standard of related regulations.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a toxin-producing medium for veterinary Clostridium novyi exotoxin, a method for preparing veterinary Clostridium novyi exotoxin by using the medium, and veterinary Clostridium novyi exotoxin Application of Clostridium exotoxin in the preparation of veterinary vaccines. Background technique [0002] Clostridial Diseases of Sheep are a group of diseases caused by microorganisms of the genus Clostridium. Including sheep rapid epidemic (Braxy, Bradsot, caused by Clostridium putrefaction Cl.septicum), sheep enterotoxaemia (Enterotoxaemia, caused by Clostridium welchii Cl. Struck (caused by Clostridium welchii Cl.perfringens type C), lamb dysentery (caused by Clostridium welchii bacteria Cl.perfringens type B), black disease (caused by Clostridium novyi Caused by), Clostridium botulinum poisoning disease (Botulism, caused by Clostridium botulinum type C, D t...

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Problems solved by technology

[0008] At present, the preparation of Clostridium novyi exotoxin is generally prepared by using beef, fish, beef liver and other animal raw materials to prepare anaerobic meat liver gastric enzyme digestion soup, cod liver meat gastric enzyme digestion soup medium, etc., using this Animal-like raw material culture medium often has unstable toxin production performance due to uneven quality of raw materials and large batch-to-batch differences. Especially in recent years, due to the development of the domestic dairy industry, the number of dairy cows has increased rapidly. As a result, a large number of eliminated dairy beef flooded the market and caused a decline in the quality of raw materials for preparing vaccine media; at the same time, residual drugs in beef and beef liver often inhibited bacterial growth, resulting in low toxin-producing performance. All of the above seriously affected vaccines. The quality of vaccines, the aggravation of cattle and sheep clostridial diseases in recent years also reflects the decline of vaccine quality

In addition, the traditional medium preparation process is cumbersome, time-consuming, requires a lot of manpower, is expensive, and the toxin-producing efficiency of the prepared medium is also low, which brings huge waste and high cost to vaccine production, and brings great harm to vaccine production enterprises. cause great distress

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