A method for the remediation of arsenic-contaminated soil by bacteria-induced active iron-manganese oxides under aerobic/microaerobic conditions
A technology of iron and manganese oxides, inducing activity, applied in the restoration of polluted soil, etc., can solve the problems of easy migration of arsenic and limited arsenic repair ability.
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Embodiment example 1
[0028] 1) Enrichment culture of bacteria
[0029] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 5%, and then aerobic enrichment culture was carried out at 25 degrees Celsius (150 rpm) for 2 days;
[0030] The components of the medium A are 0.5 g of yeast extract powder, 0.5 g of hydrolyzed casein, 0.5 g of glucose, 0.29 g of calcium chloride, 0.5 g of magnesium sulfate, 1 mL of trace elements, and 1 liter of deionized water;
[0031] The selected bacterial strains come from ATCC, the American Type Bacteria Collection Center, with the preservation number ATCC 23483;
[0032] 2) Preparation of active metabolites:
[0033] Add the Pseudomonas putida bacteria solution enriched in step 1) to the Pseudomonas putida culture medium B with an initial concentration of manganese carbonate of 1 g / L and a pH of 6.8 at an inoculum of 6% by volume, and then in 25 Celsius, cultivated under aerobic conditions for 4 day...
Embodiment example 2
[0039] 1) Enrichment culture of bacterial strains:
[0040] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 10%, and then aerobic enrichment culture was carried out at 25 degrees Celsius for 5 days under horizontal shaking (150 rpm);
[0041] The components of medium A are 0.5g of yeast extract powder, 0.5g of hydrolyzed casein, 0.5g of glucose, 0.2g of calcium chloride, 0.3g of magnesium sulfate, 4mL of trace elements, and 1 liter of deionized water;
[0042] The selected bacterial strains come from the American Type Bacteria Collection Center, with the preservation number ATCC 23483;
[0043] 2) Preparation of active iron manganese oxide:
[0044] Add the Pseudomonas putida bacteria solution enriched in step 1) to the Pseudomonas putida culture medium B containing manganese carbonate with an initial concentration of 1.0g / L and a pH of 6.8 at an inoculation volume of 10% by volume, and then in 30 degree...
Embodiment example 3
[0049] 1) Enrichment culture of bacterial strains:
[0050] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 8%, and then aerobic enrichment culture was carried out at 20 degrees Celsius (120 rpm) for 5 days;
[0051] The composition of medium B is 0.35g of yeast extract powder, 0.35g of hydrolyzed casein, 0.35g of glucose, 0.2g of calcium chloride, 0.5g of magnesium sulfate, 2mL of trace elements, and 1 liter of deionized water;
[0052] The selected bacterial strains come from the American Type Bacteria Collection Center, with the preservation number ATCC 23483;
[0053] 2) Preparation of active iron manganese oxide:
[0054] Add Pseudomonas putida culture medium B containing manganese carbonate with an initial concentration of 1.0g / L and a pH of 7.0 to the inoculum of Pseudomonas putida obtained in step 1) at a volume ratio of 7%. The ingredients are 0.25g of ferrous ammonium sulfate, 0.25g of sodium c...
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