Method for improving nickel contaminated soil remediation efficiency of plants

A technology of contaminated soil and phytoremediation, applied in the field of soil remediation, can solve the problems of destruction, soil acidification, soil physiology and biochemistry, etc., and achieve the effect of optimizing efficiency and improving efficiency

Active Publication Date: 2019-04-05
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above bacterial agents can effectively promote the accumulation of nickel in plants to repair nickel soil, there are also problems such as soil acidification and other soil physiological and biochemical damage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The test plants used in this experiment were Colombia-0 wild-type Arabidopsis. The soil was obtained by mixing nutrient soil (Klasmann-deilmannGmbh), vermiculite and perlite at a ratio of 6:3:1 (v / v), and autoclaving (121°C, 30min). The sterilized soil was placed on a plastic waterproof film and spread out to dry naturally, and the soil was added with nickel sulfate hexahydrate (NiSO 4 ·6H 2 O) solution mixing obtains containing 50mg / kg Ni soil. Both the non-polluted soil and the Ni-polluted soil were aged for three months after preparation. The physical and chemical properties of the test soil are as follows: pH 6.3, 35g / kg N, 25g / kg P, 45g / kg K.

[0025] Before the start of the test, the aged soil was homogenized again and distributed into F150 straight-sided pots (150×105×115 mm), and 200 g of the test soil was weighed in each pot for use. Spread a piece of 80-mesh nylon gauze on a sponge with a thickness of about 2 cm, infiltrate it with Hoagland's culture mediu...

Embodiment 2

[0035] In Example 1, "Colombia-0 wild-type Arabidopsis" was changed to "Type 2C protein phosphatase deletion mutant", and the rest were the same as in Example 1. The results obtained are shown in Table 2:

[0036] C-type protein phosphatase (AT4G26080, AT5G57050 and AT1G72770) deletion mutants can be obtained by relatively mature CRISPR / Cas9 gene editing methods.

[0037] Table 2 Ni content and accumulation in shoots of 2C-type protein phosphatase deletion mutants treated with 50mg / kg Ni-contaminated soil

[0038]

Embodiment 3

[0040] In Example 1, "Colombia-0 wild-type Arabidopsis" was changed to "sucrose-like non-glycolytic type 1 protein kinase (AT3G50500 and AT5G66880) deletion mutants (which can be obtained through the relatively mature CRISPR / Cas9 gene editing means) Obtain gene) ", all the other are with embodiment 1. The results obtained are shown in Table 3:

[0041] Table 3 Ni content and accumulation in shoots of Arabidopsis thaliana shoots treated with 50 mg / kg Ni-contaminated soil

[0042]

Ni

Ni+Protomicromonospora yunnanensis

Statistical Analysis

Ni contentmg / kg

20

21

Not obvious

Ni accumulation per plant μg / plant

0.4

0.5

Not obvious

[0043] In summary, the inventors have found through a lot of research that this method can promote the absorption of Ni by the plant by significantly increasing the gene expression of the nickel transporter in Arabidopsis roots, thereby promoting the efficiency of phytoremediation of soil Ni poll...

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PUM

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Abstract

The invention discloses a method for improving the nickel contaminated soil remediation efficiency of plants. The method includes steps of planting the remediation plants in to-be-remedied nickel contaminated soil; periodically inoculating bacteria solution of Promicromonospora yunnanensis into the soil at the roots of the remediation plants in the growth cycles of the remediation plants. A preservation number of the Promicromonospora yunnanensis is CGMCC 4.1290. The method has the advantages that a method for applying Promicromonospora yunnanensis activation sucrose non-glycolysis type-1 protein kinases to the Ni contaminated soil is provided, accordingly, the plants can be promoted to absorb Ni in the soil, and effects of improving the soil Ni contamination remediation efficiency of theplants can be realized.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a method for activating plant root sucrose non-glycolytic type 1 protein kinase by using Promicromonas yunnanensis to improve the efficiency of phytoremediation of nickel-contaminated soil. Background technique [0002] With the continuous advancement of my country's economic society and urbanization process, environmental pollution, especially soil pollution, is becoming increasingly serious. As of 2016, 25 million hectares of cultivated land across the country have suffered heavy metal pollution to varying degrees (Zhu Yijun et al. Chinese Society of Environmental Sciences 2016 Academic Annual Conference. 2016:4014-4018). Among them, the excessive discharge of Ni catalysts and Ni residues produced in industrial activities, sewage irrigation in agricultural production, excessive application of phosphate fertilizers, and extensive use of plastic film, etc., cause a large...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 卢琪李怀悦翁轶能攸越潘维都韶婷
Owner ZHEJIANG GONGSHANG UNIVERSITY
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