Primer for CDS (Coding Sequence) domain sequence of ACP5 (Acid Phosphatase 5) gene of clonal chicken

Abstract

The invention discloses a primer for a CDS (Coding Sequence) domain sequence of an ACP5 (Acid Phosphatase 5) gene of clonal chicken. The invention designs a group of specific primers, PCR (PolymeraseChain Reaction) amplification is carried out on the ACP5 gene of chicken by combining the primers and applying an RT (Reverse Transcription)-PCR technology, a target fragment of the PCR amplificationis a complete CDS domain sequence of the ACP5 gene of the chicken, the obtained complete CDS domain sequence of the ACP5 gene is high in accuracy and reliable in result, and basic materials are provided for constructing protokaryotic and eukaryotic expression vectors of the ACP5 gene of the chicken, related animal models and the like. According to the primer disclosed by the invention, high-GC (Guanine-Cytosine) content sequence cloning is obtained through a common cloning means; enzyme cutting sites are added to the primer, so that the primer can be directly used for other expression experiences, and meanwhile, an important laboratory significance in treating and improving bone metabolism of caged laying hens is obtained.

Description

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AI Technical Summary

Benefits of technology

This patented method allows researcher to study how different parts or organs affect blood glucose levels during developmental stages such as embryogenesis (the process where cells develop from their germ layers). By combining certain special primer sets together they created an assay called CSPC-5 that accurately detected changes caused by these factors on developing fetuses' brain tissue. These techniques are useful tools for studying various aspects of fetal growth including neural activity, neurology, muscle physiology, nutritional status, etc., making it possible to better understanding diseases associated with abnormalities like diabetes mellitus.

Problems solved by technology

The technical problem addressed by these inventions relates to identifying specific proteins called tissue-resident enzyme 2-7 (TAMP2) or acetyltransferring protein 3-58 (ATIPS), specifically involved in regulating calcium homeostasis during skeletal formation and growth processes such as embryogenesis and nerve cell migration. This helps us better predict how well healthy individuals develop degenerative joint disease like arthritis due to increased amounts of certain substances produced through degradation process caused by their own body's immune response.

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