A rotary microfluidic chip for nucleic acid extraction from biological samples

A microfluidic chip and biological sample technology, which is applied in the measurement/inspection of microorganisms, special-purpose bioreactors/fermenters, and post-processing of biomass, which can solve the problems of high cost, long cutting time, and manual extraction of nucleic acid There are many operating steps, etc., to achieve the effect of simple operation

Active Publication Date: 2021-05-11
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is that there are many manual extraction steps for nucleic acid extraction, which requires professionals to participate in the cutting and takes a long time, while the automatic extraction instrument requires a large throughput and high cost

Method used

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  • A rotary microfluidic chip for nucleic acid extraction from biological samples
  • A rotary microfluidic chip for nucleic acid extraction from biological samples
  • A rotary microfluidic chip for nucleic acid extraction from biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The composition of the lysate in the lysate well was 14.77g of guanidine isosulfate dissolved in 20mL of 0.1M Tris buffer, and then 4.4mL of 0.2M EDTA (pH=8.0) and 0.325mL of Triton X-100 (pH=6.4) were added.

[0050] The composition of the cleaning solution in the cleaning hole is an aqueous solution of 80% ethanol

[0051] The composition of the eluent in the elution well is water

[0052] The magnetic beads in the reaction wells are MagicMag Beads (Sangon Biotech)

[0053] The upper sub-chip of the spin chip is molded by PDMS. The maximum volume of the lysis hole is 1mL, the maximum volume of the cleaning hole is 1mL, and the maximum volume of the elution hole is 0.2mL. The sub-chip is made of glass material, which is completed by wet etching. Lower the magnetic bead wells on the chip to 2 µl.

[0054] The surfaces of the upper sub-chip and the lower sub-chip are assembled together through surface silanization and hydrophobization treatment, and the central axis h...

Embodiment 2

[0065] The main structure and operation mode of this example are the same as those of Example 1, the difference is that the rotary microfluidic chip in this example also includes a drying hole, and the eluted nucleic acid is further dried to better remove ethanol and other substances, the schematic diagram like figure 1 shown.

Embodiment 3

[0067] In this embodiment, there are two cleaning holes in the rotary microfluidic chip, and the magnetic beads can be washed repeatedly for many times. Different cleaning fluids can be preloaded in each cleaning hole, and the volumes can be the same or different.

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Abstract

The invention discloses a rotary microfluidic chip for nucleic acid extraction of biological samples, which is characterized in that the rotary microfluidic chip includes an upper sub-chip and a lower sub-chip, the lower surface of the upper sub-chip and the upper surface of the lower sub-chip contact, the upper sub-chip is set to rotate relative to the lower sub-chip, the upper sub-chip includes more than 2 reagent holes, the reagent holes contain the reagents required for nucleic acid extraction, the upper surface of the lower sub-chip Including reaction wells, reaction wells or reagent wells may contain magnetic beads required for nucleic acid purification. The device is easy to operate, can quickly complete nucleic acid extraction without professional technicians, can complete nucleic acid extraction in many different application scenarios, and can also be conveniently combined with downstream microfluidic analysis methods.

Description

technical field [0001] The invention relates to a rotary microfluidic chip, in particular to a rotary microfluidic chip for nucleic acid extraction of biological samples. Background technique [0002] The extraction of nucleic acids (including DNA and RNA) from biological materials is of great significance for biological research and clinical medical testing. Nucleic acid extraction has become one of the most basic operations in molecular biology experiments. Although there are many methods for nucleic acid extraction at present, the steps can basically be summarized as follows: 1. Cell lysis; 2. Precipitation or adsorption of nucleic acid; 3. Purification of nucleic acid; 4. Elution of nucleic acid. [0003] Cell lysis can include temperature lysis (such as thermal lysis), mechanical lysis (such as bead beating and grinding, etc.), ultrasonic lysis (cell membrane damage by ultrasonic waves), chemical lysis (such as adding sodium hydroxide solution), biological Cleavage (s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N15/10
CPCC12N15/1003C12N15/1013C12Q2565/629
Inventor 沈峰
Owner SHANGHAI JIAO TONG UNIV
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