Culture medium for identifying infectious microbes of erythromycin fermentation liquid, preparation method of culture medium, and method for identifying infectious microbes

Abstract

The invention discloses a culture medium for identifying infectious microbes of erythromycin fermentation liquid, a preparation method of the culture medium, and a method for identifying infectious microbes. The culture medium comprises the following raw materials of sodium chloride, a beef extract, yeast cream, glucose, peptone and magnesium sulphate. The preparation method comprises the following steps of enabling the raw materials to dissolve in distilled water, adjusting pH, dropwise adding a phenol red solution to the culture medium until the culture medium is deep red, subpackaging the deep red culture medium into clean empty test tubes, sealing the test tubes with rubber plugs or test tube caps, and performing sterilization so as to obtain the culture medium for identifying infectious microbes of erythromycin fermentation liquid. The method for identifying infectious microbes comprises the following steps of inoculating erythromycin fermentation liquid samples needing examination with the culture medium, performing culture, examining sample taking smear under a microscope, and through examining the microbe type of microbe strains on the smear, judging whether the erythromycin fermentation liquid contains the infectious microbes or not. The culture medium disclosed by the invention is definite in purpose, and coordinated and rich in nutrients, the preparation method is simple, quick, convenient and efficient, and the method for identifying the infectious microbes is simple, effective and high in accuracy degree.

Description

technical field [0001] The invention relates to a culture medium for identifying miscellaneous bacteria in erythromycin fermentation broth, a preparation method thereof and a method for identifying miscellaneous bacteria, belonging to the field of microbial detection. Background technique [0002] The culture medium method is a traditional air microbial aerosol detection method. Generally, the microorganisms are sampled on a liquid, solid or semi-solid sampling medium by natural sedimentation or a sampler, and then cultured and propagated to grow into bacteria, counted, and then separated and purified. , Through detection and identification, determine what kind of microorganism it is. [0003] Erythromycin is an alkaline antibiotic isolated from the culture medium of Streptomyces erythreus in 1952. Erythromycin has a broad-spectrum antibacterial effect, and has a strong inhibitory effect on Gram-positive bacteria, such as Staphylococcus, Streptococcus pyogenes, Streptococcu...

AI Technical Summary

Problems solved by technology

[0004] At present, domestic research on erythromycin fermentation technology has been carried out. With the production of high-yield strains and the continuous improvement of fermentation methods, the level of fermentation units has been continuously improved, but the problem of difficult identification of raw materials contaminated with bacteria has not yet been solved, resulting in Deterioration of the fermentation broth, poor quality