Application of dna tetrahedron in preparation of medicine for treating corneal injury

A technology of corneal injury and tetrahedron, which is applied in the field of ophthalmic medicine, can solve the problems of insufficient treatment effect and side effects, and achieve the effect of simple synthesis method, promoting tissue regeneration, promoting proliferation and migration

Active Publication Date: 2021-03-12
CHENGDU GENREZE GENE TECH CO LTD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But corticosteroids inhibit tissue regeneration and have other potential side effects, and the overall treatment remains inadequate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of dna tetrahedron in preparation of medicine for treating corneal injury
  • Application of dna tetrahedron in preparation of medicine for treating corneal injury
  • Application of dna tetrahedron in preparation of medicine for treating corneal injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] Preparation and Identification of Example DNA Tetrahedron

[0031] 1. Method

[0032] 1.1 Preparation method of DNA tetrahedron (TDN)

[0033] TDN is self-assembled by four uniquely designed DNA single strands (S1, S2, S3, S4) through a fast, simple and specific PCR program (95°C for 10min, rapid cooling to 4°C for 20min, long-term storage at 4°C) Synthetic. The four single strands were added to 96 μl TM buffer (10 mM Tris-HCl, 50 mM MgCl 2 , pH 8.0) in a 200μl EP tube, the reaction solution was heated to 95°C for 10min, and then rapidly cooled to 4°C to synthesize TDN.

[0034] 1.2 The specific sequences of the four DNA single strands are as follows:

[0035]

[0036] 1.3 Characterization of TDN by polyacrylamide gel electrophoresis, dynamic light scattering (DLS), atomic force microscopy (AFM), transmission electron microscopy (TEM) and charge determination:

[0037] ①DLS: The synthesized TDN was diluted to 250nM with twice distilled water and then observed on...

experiment example 1

[0043] Experimental Example 1 Cell Migration Experiment

[0044] Wound healing is a dynamic, strictly ordered biological process, in which re-epithelialization plays a very important role. Wound re-epithelialization mainly depends on the migration of epithelial cells from the wound edge to the wound center. In order to investigate whether TDN contributes to the migration of human corneal epithelial cells, the inventors conducted the following experiments.

[0045] 1. Method

[0046] The effect of TDN on the migration of human corneal epithelial cells was detected by scratch test, transwell test and RTCA method.

[0047] Scratch test: Cells were divided into 1.5×10 5 Cells / well were inoculated into 12-well plates for culture. When the cells were 80-90% full, use a sterile gun tip to draw two vertically intersecting scratches. After washing away the cell debris, add TDN at a concentration of 125nM. , 250nM, 375nM cell culture medium was the test group, and the cell culture m...

experiment example 2

[0054] Experimental Example 2 Cell Proliferation Experiment

[0055] 1. Method

[0056] To examine the effect of TDN on the proliferation behavior of corneal epithelial cells, we conducted three experiments.

[0057] CCK-8 assay: Cells were divided into 5×10 3 The density of each well was inoculated in a 96-well plate, and after using the growth medium for 24h, it was replaced with a serum-free medium containing different concentrations (0nM, 125nM, 250nM, 375nM) of TDN, at 6h, 12h, 24h, 36h, According to the cck-8 product use method, the cck-8 absorbance photometry analysis test was carried out respectively, and the statistical analysis was carried out by using SPSS.

[0058] BrdU cell proliferation test: corneal epithelial cells were treated with 1×10 4 The density of cells / ml was inoculated in a confocal small dish, and after culturing with the growth medium for 24 hours, the medium was replaced with a medium containing 10 μm BrdU and different concentrations (0 nM, 250 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

In order to solve the problem of lack of specific medicines for corneal injury at present, the present invention provides the use of DNA tetrahedron in preparing a medicine for treating corneal injury, the DNA tetrahedron is a tetrahedral nanostructure formed by four DNA single strands through base complementary pairing . The invention can effectively promote tissue regeneration, realize the healing of damaged cornea, has no toxic and side effects, and has a simple preparation process.

Description

technical field [0001] The invention relates to the field of ophthalmic medicines, in particular to the use of DNA tetrahedrons in the preparation of medicines for treating corneal damage. Background technique [0002] Corneal injury is one of the most common and difficult ocular injuries in clinical practice, mainly caused by chemical burns, and the most common type of chemical burns is alkali burns. Aseptic corneal ulcer, perforation, eyeball adhesion and secondary glaucoma after corneal injury are common causes of ocular disability. [0003] The clinical treatment of corneal injury includes timely and thorough cleaning of the conjunctival sac, subconjunctival injection of vitamin C, atropine mydriasis, local and systemic anti-inflammatory, nutritional cornea, amniotic membrane transplantation, autohemotherapy, covering the affected eye, etc., but these drugs can only Slightly alleviate the condition, the treatment effect is poor. [0004] Some studies have used corticos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/711A61P27/02
CPCA61K31/711A61P27/02
Inventor 林云锋刘楠馨张晓琳
Owner CHENGDU GENREZE GENE TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap