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Handheld animal and plant tissue ablation instrument based on ultrasonic principle

A tissue ablation, hand-held technology, applied in the field of RNA, DNA or protein extraction, can solve the problems of homogenization into smaller tissue clumps, inconvenient hand-holding of the finished structure, inconvenient installation of the structure, etc., to achieve low price, strong amplitude, and easy installation solid effect

Pending Publication Date: 2019-04-19
CHENGDU DAOSHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Common applications of ultrasonic vibrators are: ultrasonic cleaners, ultrasonic nebulizers, B-ultrasound probes, etc., but the existing ultrasonic vibrators have the problems of inconvenient structural installation and inconvenient hand-holding of the finished product structure after installation.
[0004] From the above content, it is known that there is no relevant ultrasonic vibrator application in the existing methods for rapid extraction of RNA, DNA or protein from tissues. Aiming at the rapid extraction of RNA, DNA or protein from tissues, a tissue fragmentation product produced by Thermo Company of the United States The principle of the machine: use the irregular high-speed motion of the magnetic beads in the electromagnetic field to grind the tissue into a tissue homogenate, but the machine is expensive and can only homogenize the tissue into smaller tissue clumps; the US PR0*PR0200 handheld, desktop two With the homogenizer and Bioprep-24 biological sample homogenizer high-throughput biological sample homogenization instrument, there are also expensive machines, and the tissue can only be homogenized into smaller tissue clumps

Method used

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  • Handheld animal and plant tissue ablation instrument based on ultrasonic principle
  • Handheld animal and plant tissue ablation instrument based on ultrasonic principle
  • Handheld animal and plant tissue ablation instrument based on ultrasonic principle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Such as Figure 1-10 As shown, the handheld animal and plant tissue ablation instrument based on the ultrasonic principle includes a housing 1 provided with a control board 4 and a driving board 5, and an ultrasonic vibrator fixedly connected to the front end of the housing; the ultrasonic vibrator includes an ultrasonic transducer 2 and a fixed connection The horn 3 is located at the front end of the ultrasonic transducer 2 and transmits the vibration input by the ultrasonic transducer after changing its amplitude; the control board 4 is electrically connected with the driving board 5, and the driving board 5 is electrically connected with the ultrasonic transducer 2 to control The board 4 is connected with a button 6, a display screen 7 and a charging interface; the shell 1 is a hand-held shell, and the shell 1 includes a first segment and a second segment that are mutually obtuse, and the key 6 and the display screen 7 are arranged on the surface of the second segment...

Embodiment 2

[0114] This example introduces a method for quickly extracting RNA, DNA or protein with a hand-held animal and plant tissue ablation instrument based on the principle of ultrasound, including steps: A, preparing cell suspension; B, extracting RNA, DNA or protein; Step A includes After the tissue sample is mixed with the solution, ultrasonic ablation is performed using a handheld animal and plant tissue ablation instrument based on the principle of ultrasound to obtain a cell suspension.

[0115] The method of low-hertz ultrasound is used to quickly and easily ablate the tissue block into a cell suspension, and then facilitate the subsequent extraction of tissue RNA, DNA or protein, and only need to use a small amount of tissue to achieve extraction from the tissue RNA, DNA or protein, especially the purpose of RNA and protein, is easy to operate, saves extraction time, and greatly shortens the time from tissue block to cell suspension; all operations can be completed by only on...

Embodiment approach

[0119] One embodiment, when the solution in step A is RNA extraction solution, protein extraction solution or DNA extraction solution, step B includes extracting RNA, protein or DNA respectively according to the conventional operation mode of RNA extraction solution, protein extraction solution or DNA extraction solution A step of.

[0120] When using RNA extraction solution, protein extraction solution or DNA extraction solution as a solution to obtain cell suspension by ultrasonic ablation, in the subsequent specific RNA, DNA or protein extraction steps, the RNA extraction solution and DNA extraction solution used can be used Or the operating instructions of the reagents corresponding to the protein extraction solution for subsequent extraction operations, or other existing conventional extraction methods suitable for extraction can also be used.

[0121] In another embodiment, when the solution in step A is physiological saline or buffer solution, step B includes mixing the...

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Abstract

The invention discloses a handheld animal and plant tissue ablation instrument based on an ultrasonic principle. The instrument comprises a shell and an ultrasonic vibrator, wherein the shell is internally provided with a control panel and a driving board, and the ultrasonic vibrator is fixedly connected to the front end of the shell; the ultrasonic vibrator comprises an ultrasonic transducer andan amplitude transformer which is fixedly connected to the front end of the ultrasonic transducer and transmits vibration input by the ultrasonic transducer after the amplitude of the vibration is changed; the control panel is provided with a power supply, a DCDC conversion unit, a main control unit, a DCDC power adjusting unit and a sampling unit; the driving board is provided with a driving unit, a voltage transformation unit and a resonance unit. The handheld shell comprises a first section and a second section between which an obtuse angle is formed, and the end, away from the second section, of the first section is the front end of the shell. According to the handheld animal and plant tissue ablation instrument, a low-hertz ultrasonic method is utilized to achieve the purpose of rapidly and simply ablating tissue blocks into cell suspensions, and therefore subsequent extraction of tissue RNA and DNA or protein is facilitated. The instrument has the advantages of being convenient to hold, install, maintain and dismantle, firm, great in effect and long in service life.

Description

technical field [0001] The invention belongs to the technical field of RNA, DNA or protein extraction, in particular to a handheld animal and plant tissue ablation instrument based on the principle of ultrasound. Background technique [0002] The ultrasonic vibrator is also called the ultrasonic vibrator. The ultrasonic vibrator realizes the mutual conversion of electrical energy and mechanical energy (sonic vibration) by the piezoelectric effect of piezoelectric ceramics, and is amplified by the front and rear radiation blocks with matching acoustic impedance. The ultrasonic vibrator is an ultrasonic vibration system composed of an ultrasonic transducer and an ultrasonic horn. The ultrasonic transducer is a device that can convert high-frequency electrical energy into mechanical energy. The ultrasonic horn is a passive device that does not generate vibration itself. It just changes the amplitude of the vibration input by the ultrasonic transducer and then transmits it. Imp...

Claims

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Application Information

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IPC IPC(8): C12M3/08C12M1/42
CPCC12N13/00C12N15/1003B06B1/0261B06B2201/76A61B10/02G01N1/286G01N2001/2866C12M47/06B26D7/086C12M45/02C07K1/145C12M1/42
Inventor 赵仲玖赵伟廖东升邱坤
Owner CHENGDU DAOSHENG BIOTECH CO LTD
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