Magnetic lipase nano-flower catalytic material and preparation method and application thereof

A catalytic material and lipase technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve problems such as difficult separation, reduced stability, and limited industrial application, achieving rapid separation, improved catalytic activity, and eliminating the need for Effect of the high-speed centrifugation step

Inactive Publication Date: 2019-04-19
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still many defects in the practical application of lipase as an industrial catalyst, and although the enzyme activity is improved after the nanoflowers are immobilized, it is easily affected by the external environment and the stability decreases, and there is a problem that it is difficult to separate. Greatly limit its further industrial application

Method used

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  • Magnetic lipase nano-flower catalytic material and preparation method and application thereof
  • Magnetic lipase nano-flower catalytic material and preparation method and application thereof
  • Magnetic lipase nano-flower catalytic material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Weigh 2.6g FeCl 3 , 1.2g trisodium citrate, ultrasonically dispersed in 80mL ethylene glycol. Add 3.12g of sodium acetate under stirring conditions, stir the mixture at 200r / min for 30min, pour it into the reaction kettle, and keep it warm at 200°C for 20h. Fe was obtained by separation and washing under the condition of an external magnetic field 3 o 4 nanoparticles.

[0058] (2) Weigh 1g Fe 3 o 4 The nanoparticles were poured into 80mL 2M NaOH aqueous solution, stirred continuously for 2h, and the precipitate was collected and washed. Disperse the collected precipitate in 200mL of toluene, add 0.702g of 3-aminopropyltriethoxysilane (APTES) dropwise under stirring conditions, heat to 100°C and reflux for 10h with a water separator, and obtain magnetic properties after silanization modification Silicon-based nanoparticles;

[0059] (3) Prepare 45 mL of lipase solution with a concentration of 0.1 mg / mL with buffer solution, add 5 mg of magnetic silicon-based n...

Embodiment 2

[0067] (1) Weigh 2.6g FeCl 3 , 1.2g trisodium citrate, ultrasonically dispersed in 80mL ethylene glycol. Add 3.12g of sodium acetate under stirring conditions, stir the mixture at 200r / min for 30min, pour it into the reaction kettle, and keep it at 200°C for 20h. Fe was obtained by separation and washing under the condition of an external magnetic field 3 o 4 nanoparticles.

[0068] (2) Weigh 1g Fe3 o 4 The nanoparticles were poured into 80 mL of 2M NaOH aqueous solution, stirred continuously for 2 h, and the precipitate was collected and washed. Disperse the collected precipitate in 200mL of toluene, add 0.702g of 3-aminopropyltriethoxysilane (APTES) dropwise under stirring conditions, heat to 100°C and reflux for 10h with a water separator, and obtain magnetic Silicon-based nanoparticles;

[0069] (3) Prepare a lipase solution with a concentration of 0.25 mg / mL with a buffer solution, take 45 mL of the enzyme solution and add 5 mg of magnetic silicon-based nanoparticle...

Embodiment 3

[0071] (1) Weigh 2.6g FeCl 3 , 1.2g trisodium citrate, ultrasonically dispersed in 80mL ethylene glycol. Add 3.12g of sodium acetate under stirring conditions, stir the mixture at 200r / min for 30min, pour it into the reaction kettle, and keep it at 200°C for 20h. Fe was obtained by separation and washing under the condition of an external magnetic field 3 o 4 nanoparticles.

[0072] (2) Weigh 1g Fe 3 o 4 The nanoparticles were poured into 80mL 2M NaOH aqueous solution, stirred continuously for 2h, and the precipitate was collected and washed. Disperse the collected precipitate in 200mL of toluene, add 0.702g of 3-aminopropyltriethoxysilane (APTES) dropwise under stirring conditions, heat to 100°C and reflux for 10h with a water separator, and obtain magnetic properties after silanization modification Silicon-based nanoparticles;

[0073] (3) Prepare a lipase solution with a concentration of 0.5 mg / mL with a buffer solution, take 45 mL of the enzyme solution and add 5 mg...

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Abstract

The invention relates to a magnetic lipase nano-flower catalytic material and a preparation method and an application thereof, and belongs to the field of enzyme immobilization. The preparation methodof the magnetic lipase nano-flower catalytic material is characterized in that silicon-based magnetic Fe3O4 nanoparticles are dispersed into lipase solution; metal cation inorganic salt solution is dripped into the lipase solution to obtain the magnetic lipase nano-flower; the silicon-based magnetic Fe3O4 nanoparticles are Fe3O4 nanoparticles treated by a silane coupling agent. The catalytic material is characterized in that the excellent performances of the lipase-inorganic hybrid nano-flower and the magnetic nanoparticles are fully combined together, so that the inorganic hybrid nano-flowerand the magnetic nanoparticles have wide application values in the fields of biocatalysis, biological medicine, food industry and the like.

Description

technical field [0001] The invention relates to a magnetic lipase nanoflower catalytic material and a preparation method and application thereof, belonging to the field of enzyme immobilization. Background technique [0002] Lipase is a class of enzymes that can catalyze glyceride hydrolysis, esterification, and transesterification reactions in aqueous or organic phase systems. Because of its non-toxic and harmless, green and natural, good water solubility, specific reaction, and mild catalytic environment, it has been widely used in energy, food, washing, pharmaceutical, textile and paper industries. , so it has been closely watched and studied by scholars at home and abroad. However, there are still many defects in the practical application of lipase as an industrial catalyst. In a relatively harsh environment, it is prone to decrease in stability, loss of enzyme activity or even inactivation, and it is difficult for free lipase to be reused. This greatly limits its furt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12P7/64
CPCC12N9/20C12N11/14C12P7/649C12Y301/01003Y02E50/10
Inventor 田晶任伟帆费旭李尧徐龙权王一
Owner DALIAN POLYTECHNIC UNIVERSITY
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