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Combination of alkaloids and flavonoids of semen ziziphi spinosae for preventing and treating depression and insomnia diseases

A technology of jujube seed and total alkaloids, applied in the fields of health care products and medicine, to achieve the effect of simple and clear chemical composition, clear composition and small dosage

Pending Publication Date: 2019-04-26
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Suanzaoren soaked in water is often used abroad to treat insomnia. Studies have confirmed that alkaloids, saponins, and flavonoids are all effective parts of Suanzaoren, but the sedative, hypnotic and antidepressant effects of a single effective part have certain limitations. Different effective parts have different effects and emphases, and the effect of relieving depression and calming the nerves is the result of the synergistic effect of different parts
A related patent (application number CN201010278726) discloses a drug containing 90-160 parts of total saponins in the effective parts of Suanzaoren, 12-16 parts of total alkaloids in Suanzaoren, and 0-10 parts of total flavonoids in Suanzaoren for the preparation of drugs for treating insomnia and depression. However, the results show that the effects of different parts and doses of Suanzaoren are not directly related, indicating that the pharmacological effects of Suanzaoren are not linearly related to their components and doses. in-depth study

Method used

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  • Combination of alkaloids and flavonoids of semen ziziphi spinosae for preventing and treating depression and insomnia diseases
  • Combination of alkaloids and flavonoids of semen ziziphi spinosae for preventing and treating depression and insomnia diseases
  • Combination of alkaloids and flavonoids of semen ziziphi spinosae for preventing and treating depression and insomnia diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Experimental Study on the Protective Effect of Glutamic Acid-induced PC12 Cell Injury

[0021] 1. Test material

[0022] 1.1 Drugs and reagents

[0023] Cell line: PC12 cell line (source: Rattμs norvegicμs adrenal pheochromocytoma, provided by Nanjing KGI Biotechnology Development Co., Ltd.).

[0024] Reagents: H-DMEM cell culture medium (Hyclone, Thermo Fisher Biochemical Beijing Co., Ltd.); fetal bovine serum (Hyclone, Thermo Fisher Biochemical Beijing Co., Ltd.); Yuntian), penicillin-streptomycin mixed liquid double antibody (BI company of Israel), L-glutamic acid (Klonetech, Japan). Tetramethylazolium salt (MTT), dimethyl sulfoxide (DMSO) (pokeweed mitogen, Sigma company).

[0025] Drugs: total alkaloids, total flavonoids, total saponins (made in the laboratory)

[0026] 1.2 Test equipment

[0027] Ultra-clean bench (SW-CJ-2G, Suzhou Purification); carbon dioxide incubator (HF160W, Heal Force Company); desktop centrifuge (B600, Baiyang Centrifuge Factory); micr...

Embodiment 2

[0039] Pharmacodynamic experiments of different drug compatibility compositions on CUMS depression and insomnia mouse model

[0040] 1 Experimental materials

[0041] 1.1 Experimental animals

[0042] Healthy male ICR mice (weight 20±2g) were purchased from the Experimental Animal Center of the Institute of Radiological Medicine, Chinese Academy of Medical Sciences; they were bred for 3 days to adapt to the environment before the experiment. The room temperature is 22±2°C, and the relative humidity is 65% to 70%. Sunlight for 12 hours, free diet and water intake.

[0043] 1.2 Reagents and medicines:

[0044] Drugs: jujube seed saponins, alkaloids, flavonoids (laboratory self-made)

[0045] 2 Experimental methods

[0046] 2.1 Animal grouping and administration

[0047] The mice were randomly divided into 16 groups, 10 in each group, respectively blank group, model group, venlafaxine group, Jieyu Anshen granule group, alkaloid+flavone group (low dose), alkaloid+flavone gro...

Embodiment 3

[0092] Pharmacodynamic effects of alkaloid-flavonoids in different proportions on CUMS depression and insomnia mice

[0093] 1 Experimental materials

[0094] 1.1 Experimental animals

[0095] Same as Example 1

[0096] 1.2 Reagents and medicines:

[0097] Drugs: alkaloids and flavonoids of Suanzaoren (laboratory self-made)

[0098] 2 Experimental methods

[0099] 2.1 Animal grouping and administration

[0100]The mice were randomly divided into 13 groups, 10 in each group, and the blank group, western medicine positive group, Chinese patent medicine positive group and each drug administration group were respectively set up. Except for the blank group, the mice in the other groups were all modeled according to the method described in Example 1. From the first day of model building, they were administered orally at 8:00-9:00 every morning, once a day, and continued for 21 days. The grouping and dosing regimen are shown in Table 9.

[0101] Table 9 Animal grouping and dos...

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Abstract

The invention discloses a semen ziziphi spinosae effective part composition for preventing and treating depression and insomnia diseases, and belongs to the field of traditional Chinese medicines. Thecomposition is prepared from total alkaloids and total flavonoids extracted from semen ziziphi spinosae according to a certain ratio. In-vitro and in-vivo pharmacological experiments show that the traditional Chinese medicine effective part composition has an obvious effect of preventing and treating the depression and insomnia diseases, the curative effect of the composition is superior to thatof any single component and other effective part composition, and a good synergistic effect is achieved. The semen ziziphi spinosae effective part composition has no obvious toxic and side effects, isremarkable in curative effect on depression and insomnia and has a wide practical application value.

Description

technical field [0001] The invention belongs to the fields of medicine and health care products, and relates to a combination of jujube seed alkaloids and flavonoids and its application in treating depression and insomnia. Background technique [0002] With the acceleration of the pace of life and the increase of life and work pressure, central nervous system diseases such as depression and insomnia have become common and frequently-occurring diseases that affect human health besides cardiovascular disease, diabetes and tumors. Relevant epidemiological surveys show that the prevalence of chronic insomnia is between 6% and 30%, depression and insomnia often occur at the same time, and 60% to 80% of patients with depression have insomnia symptoms, or insomnia is a prodromal symptom; Insomnia can significantly increase the incidence of depression in subjects, and the prevalence of depression in patients with insomnia symptoms is 3 to 4 times higher than that of non-insomnia pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P25/24A61P25/20
CPCA61P25/20A61P25/24A61K36/725A61K2236/333A61K2236/331A61K2236/55A61K2236/51A61K2236/39A61K2300/00
Inventor 乔卫段宏泉孙燕张晓梅孙丹晨徐浩
Owner TIANJIN MEDICAL UNIV
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