Mixed bacteria system, method for decomposing hemicellulose using same and method for producing alcohol

A technology of hemicellulose and alcohol, applied in the fields of biochemical equipment and methods, enzymes, hydrolase, etc., to achieve the best production efficiency and the effect of improving the biomass alcohol production process

Active Publication Date: 2021-02-23
朱一民 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mixed bacteria system of the present invention can simultaneously overcome the aforementioned problems of hemicellulose hydrolysis, pentose sugar consumption, and the survival rate of recombinant yeast, and provide a high-yield and low-cost biomass alcohol production process

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  • Mixed bacteria system, method for decomposing hemicellulose using same and method for producing alcohol
  • Mixed bacteria system, method for decomposing hemicellulose using same and method for producing alcohol
  • Mixed bacteria system, method for decomposing hemicellulose using same and method for producing alcohol

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Embodiment Construction

[0025] The main method of the hemicellulolytic method provided by the present invention is to divide the yeast into several groups, wherein genes of different hemicellulolytic enzymes and anchor proteins can be fused and inserted into the genome of the yeast respectively, so that these groups Secretory expression of fusion proteins with different hemicellulolytic enzymes. The expressed fusion protein has an anchor protein part, which will be inserted into the surface of the yeast when it contacts the surface of the yeast, so that the hemicellulolytic enzyme is also fixed on the surface of the yeast. On the other hand, the remaining group can insert exogenous genes to express enzymes with five-carbon sugar fermentation function. Next, yeasts of all the above groups were co-cultured to form a mixed bacteria system. When the biomass material containing hemicellulose is added to the mixed bacteria system, the hemicellulose can be decomposed into five-carbon sugar by hemicelluloly...

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Abstract

The invention provides a method for producing biomass alcohol, which comprises recombining at least three groups of yeasts to obtain at least three groups of recombinant yeasts, wherein the recombinant yeasts contain hemicellulose-transformed bacteria expressing fusion proteins different from each other, The fusion protein is a combination of ankyrin and different hemicellulolytic enzymes among different groups, and the ankyrin has the ability to anchor to the surface of hemicellulose-transformed bacteria; and the five-carbon sugar fermentation enzyme expresses the combination of carbon sugar fermenting bacteria; co-cultivating the at least three groups of recombinant yeast; making hemicellulose contact with the hemicellulolytic enzyme to obtain five-carbon sugar; and making the five-carbon sugar fermentation enzyme convert the five-carbon sugar into biomass alcohol.

Description

technical field [0001] The present invention relates to a method for decomposing hemicellulose. Specifically, the present invention relates to a mixed bacteria system, a method for decomposing hemicellulose using it and a method for producing alcohol. Background technique [0002] In the industrial development of biomass ethanol, the currently rapidly developing consolidated bio-process (CBP) integrates cellulolytic enzymes and alcohol fermentation in the same reactor, thereby effectively reducing production costs. However, regardless of any manufacturing process, biomass raw materials must first be converted into monosaccharides through hydrolysis before further fermentation can continue. However, the hemicellulose contained in the biomass raw material needs to be hydrolyzed optimally in an acid environment, high temperature or high pressure environment in the prior art. However, in the CBP process, cellulose hydrolysis (including cellulose, hemicellulose, etc.) and alcoh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10C12N1/19C12N9/42
CPCC12N1/16C12N9/2437C12P7/10C12P19/02C12P19/14C12Y302/01004Y02E50/10
Inventor 朱一民魏毓宏蔡伸隆
Owner 朱一民
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