Mixed bacteria system, method for decomposing hemicellulose using same and method for producing alcohol

Abstract

The invention provides a method for producing biomass alcohol, which comprises recombining at least three groups of yeasts to obtain at least three groups of recombinant yeasts, wherein the recombinant yeasts contain hemicellulose-transformed bacteria expressing fusion proteins different from each other, The fusion protein is a combination of ankyrin and different hemicellulolytic enzymes among different groups, and the ankyrin has the ability to anchor to the surface of hemicellulose-transformed bacteria; and the five-carbon sugar fermentation enzyme expresses the combination of carbon sugar fermenting bacteria; co-cultivating the at least three groups of recombinant yeast; making hemicellulose contact with the hemicellulolytic enzyme to obtain five-carbon sugar; and making the five-carbon sugar fermentation enzyme convert the five-carbon sugar into biomass alcohol.

Description

technical field

[0001] The present invention relates to a method for decomposing hemicellulose. Specifically, the present invention relates to a mixed bacteria system, a method for decomposing hemicellulose using it and a method for producing alcohol. Background technique

[0002] In the industrial development of biomass ethanol, the currently rapidly developing consolidated bio-process (CBP) integrates cellulolytic enzymes and alcohol fermentation in the same reactor, thereby effectively reducing production costs. However, regardless of any manufacturing process, biomass raw materials must first be converted into monosaccharides through hydrolysis before further fermentation can continue. However, the hemicellulose contained in the biomass raw material needs to be hydrolyzed optimally in an acid environment, high temperature or high pressure environment in the prior art. However, in the CBP process, cellulose hydrolysis (including cellulose, hemicellulose, etc.) and alcoh...

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