Methods for modulating production profiles of recombinant proteins
A technology of recombinant protein and composition, which is applied in the field of cell culture and can solve problems such as quality reduction
Inactive Publication Date: 2019-05-03
ARES TRADING SA
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, adding compounds during culture, while potentially increasing potency, often reduces quality
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0061] Materials and Methods
[0062] I. Cells, Cell Expansion and Cell Growth
[0063] 1) cells
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The present invention relates to methods and compositions for culturing a host cell expressing a recombinant protein in a cell culture medium supplemented with feeds comprising effective amounts of valeric acid, whereby viability of the cells and production of said protein are increased relative to cells grown with feeds comprising no valeric acid.
Description
technical field [0001] The invention belongs to the field of cell culture. In particular, the present invention relates to methods for culturing host cells expressing recombinant proteins in a cell culture medium supplemented with a feed comprising an effective amount of valeric acid such that the viability of the host cells and the protein The production was higher than that of cells cultured without valeric acid feed. Background technique [0002] For the past 20 years, biopharmaceutical companies have focused on finding compounds that promote cell growth and recombinant protein productivity. Many authors have reported positive effects of small molecule additives on productivity, usually because they arrest cell cultures in the G0 phase [1], which is the most important phase for production. Of these, carboxylic acids had the greatest impact on the performance of the culture. They were found to inhibit HDAC1 activity (histone deacetylase 1) [2]. However, adding compound...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12P1/00C07K16/18
CPCC12N5/0018C12N2501/999C12N2510/02C12P21/02C07K16/28C07K2317/14C12N2511/00C12N5/0682C07K14/52C12N15/85C07K14/475C12P21/00C07K14/575C12N2015/8518
Inventor J-M·比尔瑟C·德斯莫格特
Owner ARES TRADING SA