Construction method for nasopharynx cancer clinic related radioresistance cell strain

A construction method and cell line technology, applied in the field of cell biology, can solve the problems of non-representative characteristics of radioresistant cell lines and insufficient clinical practice guidance, and achieve the effect of balancing the total irradiation dose and short construction period

Pending Publication Date: 2019-05-10
台州市中心医院
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Problems solved by technology

[0007] Literature: Yao Wenhu, Zhang Longzhen, Qiu Xiangnan, Zhang Wei, Li Hao, Qin Zhaohui. A method for constructing radiation-resistant cell lines of lung cancer. CN104962548A[P]. 2015. Six-well plate was selected as the cell carrier, and a gradient However, the fractionation dose adopted by this method far exceeds the conventional fractionation dose in clinical practice, and the characteristics of the radioresistant cell lines constructed by it are not representative, which is not enough to guide clinical practice

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  • Construction method for nasopharynx cancer clinic related radioresistance cell strain
  • Construction method for nasopharynx cancer clinic related radioresistance cell strain
  • Construction method for nasopharynx cancer clinic related radioresistance cell strain

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] A method for constructing a clinically relevant radioresistant cell line of nasopharyngeal carcinoma, comprising the following steps:

[0027] Step 1, the cultivation of CNE-2 cells: as figure 1 , CNE-2 cells were placed in RP-MI 1640 culture medium containing 5% FBS, routinely cultured in a 37°C / 5% CO2 cell incubator, digested and passaged with 0.25% trypsin;

[0028] Step 2, construction of CNE-2R cell line: adopt 6MV X-ray irradiation, the source sk...

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Abstract

The invention discloses a construction method for a nasopharynx cancer clinic related radioresistance cell strain. The method comprises the following steps of culturing a CNE-2 cell, and constructinga CNE-2R cell strain, wherein according to the irradiation mode, irradiation is completed according to the gradient irradiation mode that 0.5Gy*5 times, 1.0Gy*5 times, 1.5Gy*5 times, 2.0Gy*30times andaccumulated 75Gy. According to the construction method for the nasopharynx cancer clinic related radioresistance cell strain, the method adopts irradiation with the partition dosage being not largerthan 2.0Gy, is close to clinic practice, and can better reflect the actual clinic condition; compared with conventional, repeated and other irradiation modes, the method adopts the gradient irradiation mode, the total irradiation dosage, irradiation frequency and construction period are better balanced, and the construction period is short.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a method for constructing a clinically relevant radiation-resistant cell line of nasopharyngeal carcinoma. Background technique [0002] Restricted by the complexity of nasopharyngeal structure and the invasive growth characteristics of nasopharyngeal carcinoma, radiotherapy is the main treatment. With the widespread application of new technologies such as IMRT, the local control rate of nasopharyngeal carcinoma has been significantly improved, but some patients still experience tumor recurrence, mainly in T3 and T4 patients, and the 5-year local recurrence rate reaches 11.2% and 18.9%. . However, recurrent nasopharyngeal carcinoma is often treated poorly, and the 5-year survival rate is only 38%. [0003] The resistance of tumor cells to radiation is one of the main reasons for tumor residual and recurrence after radiotherapy. How to identify patients with radiotherapy-r...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N1/36C12Q1/02
Inventor 吴波应申鹏王勇丁笑笑朱杰陈卫军孙华丽丁燕倪玲琴
Owner 台州市中心医院
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