Nonsense mutation read-through activity application of 3,3'-bis-indolymethane

Abstract

The invention discloses a nonsense mutation read-through activity application of 3,3'-bis-indolymethane. Preliminary experimental results show that 3,3'-bis-indolymethane can partially recover expression of luciferase with nonsense mutation in a cell model and can partially recover expression of an ATM gene containing nonsense mutation in the extracellular protein truncation experiment, and is expected to serve as a lead compound; new drugs can be developed through structure transformation and modification, various diseases caused by nonsense mutation are treated. 3,3'-bis-indolymethane has potential medical application.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of nonsense mutation read-through activity of 3,3'-diindolylmethane. Background technique [0002] Studies have shown that 30% of the diseases caused by human gene mutations are caused by nonsense mutations. Nonsense mutations can cause stop codons to appear early in the coding region during protein translation, causing peptide chain synthesis to terminate, resulting in non-functional or Insufficient truncated protein. At the same time, the premature stop codon will also prolong the time of mRNA staying in the nucleus, and even through the nonsense-mediated mRNA decay (NMD) pathway, the mRNA is selectively degraded and the encoded protein is lost. Therefore, nonsense mutations can lead to the occurrence of numerous tumor diseases and genetic diseases. Research data show that about 12% of genetic diseases are caused by nonsense mutations, involving more than 1,800 d...

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Problems solved by technology

[0005] In view of the problems of the existing nonsense mutation read-through agents such as few types, high toxicity, and low read-through efficiency, the present invention proposes the use of 3,3'-diindolylmethane nonsense mutation read-through activity

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