Detecting method for accurately and rapidly detecting target superbacteria

A super bacteria, detection target technology, applied in the medical and health field, can solve the problems of hidden dangers to the health of patients and medical staff, difficult to completely remove, etc., to achieve rapid and accurate detection and judgment, good application value, and reduce the effect of operation difficulty.

Pending Publication Date: 2019-05-14
希拓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They can exist in the hospital environment for a long time, and can colonize the respiratory tract of medical staff. It is difficult to completely remove them with conventional disinfection measures, so they bring serious health risks to patients and medical staff.

Method used

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  • Detecting method for accurately and rapidly detecting target superbacteria
  • Detecting method for accurately and rapidly detecting target superbacteria
  • Detecting method for accurately and rapidly detecting target superbacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] In this embodiment, the qualitative detection and determination of super bacteria using fluorescence luminescence characteristics is used as an example, and the relevant detection process is briefly introduced as follows.

[0053] It should be noted that, in order to further prove the specificity of phages, the samples prepared during the experiment were mixed samples (that is, the mixed samples were a mixture of multiple super bacteria).

[0054] (1) Mixed samples and enriched culture

[0055] The common superbug standard samples (screened, identified and stored in advance) were inoculated into sterile LB medium (containing 16ug / ml of carbapenem antibiotic imipenem), and the mixed culture was used as a mixed sample.

[0056] In this example, during mixed culture, the general culture conditions are: 37°C, 200rpm shaking culture until the super bacteria concentration is not less than 10 6 CFU / ml;

[0057] The common target super bacteria, that is, the super bacteria i...

Embodiment 2

[0070] In this example, the construction of a semi-quantitative detection method for a specific superbacterium pan-drug resistant Acinetobacter baumannii AB09V (corresponding to phage ZZ1) is taken as an example, and the relevant detection process is briefly introduced as follows.

[0071] (1) Sampling and preparing mock samples

[0072] Use a planktonic air sampler to collect planktonic bacteria in the environment as a background sample (the background sample does not contain super bacteria), and add different concentrations in the early stage (the addition amount is 10 1 , 10 2 , 10 3 , 10 4 , 10 5 , 10 6 CFU / ml) pan-drug resistant Acinetobacter baumannii AB09V was prepared as a mock sample containing super bacteria (the sampling time reflects the added concentration of Acinetobacter baumannii AB09V), and preliminarily enriched in sterile LB medium , 37°C, 200rpm shaking culture, and sampling every 1h in the subsequent experiments to investigate the influence of differe...

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Abstract

The invention belongs to the technical field of medical treatment sanitation, and particularly relates to a detecting method for accurately and rapidly detecting target superbacteria. According to themethod, floating bacteria in the environment are adopted as a sample collecting object; the method comprises the specific steps of sampling, enriching, screening culture, qualitative or quantitativemeasuring evaluation and the like. By means of the characteristic that phage can split ATP released by bacteria in the survival status so that luciferase catalyzes fluorescein to emit light, the typeor content of 'superbateria' can be visually qualitatively or quantitatively determined; due to the specificity of phage, no isolated culture is needed even in a sample containing mixed bacteria, rapid and accurate detection and determination can be achieved, and therefore the good application value is achieved.

Description

technical field [0001] The application belongs to the technical field of medical and health care, and in particular relates to a detection method for accurate and rapid detection of target super bacteria. Background technique [0002] "Superbugs" is a general term for a class of bacteria, which are characterized by strong resistance to most antibiotics and can spread among humans, animals and the environment, making it more difficult to treat infected people and lowering the efficiency of treatment , the fatality rate has risen, and medical expenses have risen sharply, especially for patients with low immunity, long-term use of antibiotics, and invasive procedures among hospitalized patients. According to the "Global "Superbug" Surveillance Report released by the World Health Organization in 2014, the mortality rate of patients infected with "superbugs" is about twice that of patients infected with non-resistant bacteria. Due to the widespread application and indiscriminate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/66C12Q1/06C12Q1/04
Inventor 靳静陈松建王书伟王山梅王小亭李亚辉张改李振江张杰
Owner 希拓生物科技有限公司
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