A "fluorescence-stable isotope" dual-mode detection method for p-trinitrotoluene

A stable isotope, trinitrotoluene technology, used in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of difficult long-term stable and accurate quantitative analysis, limited photobleaching effect and matrix effect, etc., to achieve high-sensitivity detection. , long-term stability and high sensitivity detection, the effect of small matrix effect
CN109765203BActive Publication Date: 2021-07-16SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN UNIV
Publication Date
2021-07-16

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Abstract

The purpose of the present invention is to provide a "fluorescence-stable isotope" dual-mode detection method for p-trinitrotoluene (TNT). The assay consists of a polythymidine‑nucleic acid aptamer probe (AptTNT‑T30) and dual-modality detection with fluorescence detection and stable isotope detection. The principle of the present invention is: the TNT nucleic acid aptamer (AptTNT) sequence can be specifically combined with the target TNT molecule for terminal protection, avoiding exonuclease cutting, and the polythymine sequence (T30) at the 5' end can be used as a template Synthesis of copper nanoparticles. The synthesized copper nanoparticles can be detected in dual modes: in the fluorescence detection mode, rapid fluorescence detection can be performed; after the copper nanoparticles are digested in nitric acid, in the stable isotope detection mode, the inductively coupled plasma mass spectrometer can be used for highly sensitive and stable quantitative analysis , which can respectively satisfy the on-site rapid detection and highly sensitive and accurate detection of TNT.
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Description

technical field

[0001] The invention belongs to the detection field of analytical chemistry, relates to the field of Fluorimetry and stable isotope (Metal stable isotope) sensing of explosives, and particularly relates to an in-situ detection method based on polythymine deoxyribonucleic acid (Poly-T ssDNA) as a template Synthetic Copper Nanoparticles (CuNPs) Fluorescence-Stable Isotope Bimodal Paratrinitrotoluene (TNT) Detection Method. Background technique

[0002] Trinitrotoluene (TNT), as one of the most typical chemical explosives, has been widely used in military, mining, anti-terrorism and civilian fields. At the same time, TNT is a highly toxic substance, which poses a serious toxicological threat to animals, plants and humans. The serious pollution of TNT to soil and water caused by widespread application threatens the survival of ecosystems. In addition, the increasingly severe global security situation and anti-terrorism requirements are becoming more and more im...

Claims

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