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A kind of method for preparing quinic acid

A quinic acid and tannase technology, applied in the separation/purification of carboxylic acid compounds, organic chemistry, fermentation, etc., can solve the problems that quinic acid has not been found, and achieve the effect of stable quality

Active Publication Date: 2021-12-10
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the isolation of quinic acid from C.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1.0 kg of fresh leaves of the hook cone, chop finely, add ethanol / water solution equivalent to 8 times the weight of the raw material (wherein ethanol accounts for 60 v / v%), extract at 50 ° C for 2.5 hours, and filter; add 8 times the weight of the raw material to the filter residue Ethanol / water solution (of which ethanol accounts for 60 v / v%), extract at 50°C for 2.5 hours, filter; add 8 times the weight of raw material to the ethanol / water solution (of which ethanol accounts for 60 v / v%), extract at 50°C for 2.5 hours , filter, and combine the filtrates for 3 times to obtain the extract solution. After recovering the ethanol, the extract solution is filtered to remove the residual insoluble matter and collect the filtrate; Ethylene-type macroporous adsorption resin (D101) for adsorption, first use 3 times the column volume of deionized water, then elute with 4 times the column volume of ethanol / water solution (in which ethanol accounts for 30 v / v%), 0.2 times the...

Embodiment 2

[0036] Repeat Example 1, the difference is:

[0037] 1) The polar solvent used for extraction is ethanol / water solution (wherein ethanol accounts for 80 v / v%);

[0038] 2) The addition of tannase is 0.6g;

[0039] 3) The polystyrene type macroporous adsorption resin used is Diaion HP-20.

[0040] Finally, 33.3 g of quinic acid (purity 94.8%) was obtained.

Embodiment 3

[0042] Repeat Example 1, the difference is:

[0043] 1) The raw material is the bark of the bark of the cone after crushing, and the polar solvent used for extraction is methanol / water solution (wherein methanol accounts for 60v / v%);

[0044] 2) The addition of tannase is 1.0g;

[0045] 3) The model of the polystyrene macroporous adsorption resin used is AB-8.

[0046] Finally, 34.1 g of quinic acid (purity 93.4%) was obtained.

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Abstract

The invention discloses a new method for preparing quinic acid, which specifically uses hook cone as raw material, extracts with polar solvent as solvent, enzymatically hydrolyzes with tannase, and finally passes through a polystyrene macroporous adsorption resin column Obtained by chromatography; wherein, the polar solvent is a composition of water and one or two or more selected from methanol, ethanol and acetone, and the proportion of the water is ≥ 20v / v%; the The model of the polystyrene type macroporous adsorption resin is D101, Diaion HP-20 or AB-8; The eluent used during chromatography is water and one or more than two selected from methanol, ethanol and acetone The composition, the proportion of the water is ≥ 20v / v%. In the present invention, for the first time, quinic acid with stable quality, high yield and high purity is obtained by extracting, hydrolyzing with tannin enzyme, and high-purity quinic acid by using the leaves of the quinic acid as a raw material, which not only provides a new supply raw material for the preparation of quinic acid, It also opens up a new utilization path for the hook cone.

Description

technical field [0001] The invention relates to a method for preparing quinic acid, in particular to a novel method for preparing quinic acid. Background technique [0002] Quinic acid is an alicyclic organic acid unique to higher plants and a precursor for the biosynthesis of aromatic amino acids. It is commonly found in vascular plants and often coexists with shikimic acid in plants. Quinic acid has a special structure and has multiple chiral centers. It is an important material in three-dimensional organic synthesis. It is widely used as a chiral raw material to synthesize natural products and prepare new polymer materials. Its specific structure is shown in the following formula: [0003] . [0004] hook cone ( Castanopsis tibetana Hance ) is a Fagaceae and Cone plant with thick bark, cracked like pine bark, and the inner bark is light reddish brown, almost smooth. Ring porous wood, the xylem only has thin wood rays, the heartwood and sapwood are distinct, the heart...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/42C07C51/42C07C51/47C07C62/04
Inventor 黄永林王亚凤颜小捷何瑞杰李典鹏卢凤来陈月圆王磊李连春
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI