50 results about "Quinamic acid" patented technology

The invention discloses a method for preparing a high purity dicaffeoylquinic acid compound by high-efficient separation, mainly comprising: using an alcohol-water solution to extract taraxacum fresh plant or dry plant, carrying out the column chromatographic separation after the concentration, preparing a crude extract rich in dicaffeoylquinic acid compound, then isolating and purifying the crude extract by high-speed countercurrent chromatography, and finally adopting a recrystallization process for refining so as to obtain 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid with high purity. The method is applied to the preparation of high purity monomers by taking various natural products or natural product extracts containing one or a plurality of ingredients in the dicaffeoylquinic acid compound and acquired through various approaches as raw materials, and has advantages of simple steps, simple operation, high efficiency, low cost, easy repetition, and large output of separation and preparation. The purity of dicaffeoylquinic acid compound prepared by the method can reach 98 percent.

The invention discloses an HPLC (High Performance Liquid Chromatography) method for simultaneously determining the contents of seven components in blumea balsamifera. Seven caffeoyl quinic acid components determined by the method are 5-O-caffeoyl quinic acid, 3-O-caffeoyl quinic acid, 4-O-caffeoyl quinic acid, 1,3-O-dicaffeoyl quinic acid, 3,4-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acidand 4,5-O-dicaffeoyl quinic acid. The HPLC method comprises the following determining steps of (1) preparation of a standard solution; (2) preparation of a tested solution; (3) establishment of content determination standard curves of seven caffeoyl quinic acid components; (4) determination of the contents of seven caffeoyl quinic acid components in a sample. According to the HPLC method disclosed by the invention, the operation is simple and convenient, the precision degree, the repeatability and the stability are good, the accuracy is high, the contents of seven caffeoyl quinic acid components in the blumea balsamifera can be simultaneously determined, and higher practical value is obtained.

A honeysuckle preparation takes traditional Chinese medicine honeysuckle as raw material, and is characterized in that the proportion of mono-caffeic acyl quinic acid to bi-caffeic acyl quinic acid in the honeysuckle preparation is 1: 0.5-1.5; the mono-caffeic acyl quinic acid comprises: chlorogenic acid and / or 5-caffeic acyl quininic acid and / or 4-caffeic acyl quininic acid and / or caffeic acid; the bi-caffeic acyl quinic acid comprises: 3, 4-bi-caffeic acyl quininic acid and / or 3, 5-bi-caffeic acyl quininic acid and / or 4, 5-bi-caffeic acyl quininic acid. The processing method lof the honeysuckle preparation are as follows: mixing the honeysuckle with honey evenly, at 100-220 DEG C, steaming for 0.5-24 hours; the weight of the honey is 0-5 times of the honeysuckle, and the concentration is 0-70 percent; drying at the normal pressure and at 10-100 DEG C, and then, the honeysuckle preparation is prepared. The invention also relates to an application of the honeysuckle preparation on preparing the drink or drugs for removing pathogenic heat from blood, activating blood and dissolving stasis. The invention changes the component shapes of the honeysuckle, thus removing pathogenic heat from blood, activating blood and dissolving stasis. The application range of the honeysuckle is enlarged.

The invention belongs to the field of organic acid extraction and separation, and relates to a method for extracting shikimic acid and quinic acid from ginkgo leaves. The problems of long consumed time, large wastewater treatment capacity, environmental pollution, complicated operation and the like in the existing method are solved; the chemical properties of each component in the ginkgo leaves are compared; a carbon dioxide supercritical extraction-extraction-membrane filtration-electrodialysis-crystallization method is combined for use; on the premise of commercial value, various active ingredients, including chlorophyll, ginkgetin, bilobalide, gingko polysaccharide and the like, in the ginkgo leaves are developed to the maximum extent, and finally, shikimic acid and quinic acid productsare obtained through separation, and the content of the shikimic acid and the content of the quinic acid are respectively greater than 98%.

The invention relates to a method for manufacturing phenolic acid positions of vernonia anthelmintica and anti-inflammation application of the phenolic acid positions. The method includes smashing dried fruits of the vernonia anthelmintica; extracting extracts by the aid of organic solvents; separating and purifying the extracts by the aid of resin to obtain the phenolic acid positions of the vernonia anthelmintica. Main active ingredients of phenolic acid at the positions of the vernonia anthelmintica include 3, 4-dicaffeoyl-quinic acid, 3, 5-dicarffeoyl-quinic acid and 4, 5-dicaffeoyl-quinic acid, the total content of the phenolic acid ranges from 20% to 99%, excess release of nitric oxide of inflammation mediators can be obviously suppressed by the phenolic acid positions of the vernonia anthelmintica in inflammation response of mice macrophage RAW264.7 induced by lipopolysaccharide, excessive secretion of interleukin 6, interleukin 1 and tumor necrosis factors alpha of inflammation factors can be suppressed, excess expression of nitric oxide synthase and epoxidase 2 can be suppressed, and phosphorylation of nuclear factors kB can be suppressed. The method has the advantages that the phenolic acid positions of the vernonia anthelmintica has excellent anti-inflammation activity and can be used for preventing or treating inflammation due to the excess nitric oxide, the excess tumor necrosis factors alpha, the excess interleukin 6 or the excess interleukin 1.

The invention relates to the technical field of identification of medicinal materials and especially relates to a method for identifying Polygonum bistorta L., Polygonum viviparnm L. and Polygonum paleaceum Wall.. The method takes three components, that is, chlorogenic acid, 3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid, as distinguishing substances. The method is simple and easy tooperate, short in analysis time and high in accuracy reaching 100%, and is suitable for being widely promoted.

The invention discloses a method for extracting shikimic acid and quinic acid from ginkgo biloba extract adsorption column-discharging water, wherein the method comprises the steps: taking ginkgo biloba extract adsorption column-discharging water concentrated solution with solid content of 60-90%, and carrying out alcohol precipitation on the concentrated solution by using 90-95% short carbon chain alcohol, wherein the ratio of the volume of short carbon chain alcohols to the mass of the concentrated solution is (4-5):1; filtering to obtain a shikimic acid filtrate and a quinic acid crude extract; drying and concentrating the shikimic acid filtrate to obtain a shikimic acid crude extract; dissolving the shikimic acid crude extract by using 85-95% ethanol, crystallizing, and drying to obtain shikimic acid with the purity of more than or equal to 98%; and dissolving the quinic acid crude extract by using 85-95% methanol, crystallizing, and drying to obtain quinic acid with the purity ofmore than or equal to 98%. Quinuclidic acid and shikimic acid in the ginkgo biloba extract preparation waste liquid can be extracted at the same time, operation is easy and convenient, the purification effect is good, and the method is suitable for industrial production and good in practicability.

The invention provides an engineering strain for reducing by-products quinic acid and glutamic acid in the process of biosynthesizing shikimic acid and application of the engineering strain, and belongs to the technical field of bioengineering.According to the engineering strain, an escherichia coli recombinant strain HYY1458 serves as an initial strain, quinic acid dehydrogenase (ydiB) is knocked out, so that the synthetic route of quinic acid is blocked, and the yield of the shikimic acid is increased; glutamate synthetase (gltB) and glutamate dehydrogenase (gdhA) are knocked out to partially hinder a glutamic acid synthesis route, the constructed strain is named as an escherichia coli engineering strain YY1127, the escherichia coli engineering strain YY1127 is used for fermentation culture, the content of quinic acid and glutamic acid is reduced by 85.3%, the purity of biosynthesized shikimic acid is improved, and the yield of the shikimic acid is increased. The subsequent separation and purification difficulty is reduced.

The present invention discloses a method for determining the content of organic acid metabolites of black plum in colon tissue and its application. The determination method comprises the following steps: (1) adding sterile physiological saline to the colon tissue, grinding, adding methanol to continue grinding, centrifuging, collecting the supernatant, adding methanol and vortexing, centrifuging, and finally adding water and vortexing to obtain a sample solution to be tested; (2) using high performance liquid chromatography-mass spectrometry to draw standard curves of the three organic acids using citric acid, malic acid, and quinic acid standards, wherein citric acid is used in positive ion mode, and malic acid and quinic acid are used in negative ion mode; (3) using the same method to detect the sample to be tested, and the concentration or content of the three organic acid metabolites (citric acid, malic acid, and quinic acid) can be calculated based on the standard curve. The method of the present invention is simple and highly sensitive and can be used to study the mechanism of the effect of black plum on ulcerative colitis.

The invention relates to the technical field of electrolyte additives, in particular to an electrolyte additive for improving the low-temperature stability of vanadium batteries and a preparation method thereof, which comprises the following components: 3-5 parts of lauric acid, lauryl sulfosuccinic acid mono 1-2 parts of disodium ester, 0.3-0.5 parts of disodium sulfosuccinate monoester, 5-8 parts of monolauryl phosphate, 1-2 parts of quinic acid, quaternary ammonium 3-5 parts of salt, 10-15 parts of sulfuric acid solution. The richness of the admixture group of the present invention can improve the steric hindrance effect, reduce the generation of precipitation, improve the stability of the electrolyte, have a significant effect on the improvement of the fluidity of the electrolyte, and can enhance the action time of the electrolyte during use. Improve the continuous use effect of the battery.

The present application relates to plant active ingredient extraction technology, particularly to a method for extracting quinic acid in cranberry, specifically, the method comprises the following steps: crushing the cranberry, mixing the crushed cranberry with water to form a slurry, extracting the slurry under low temperature and ultrasonic wave, filtering the slurry after the extraction is completed to remove solid residues, obtaining a filtrate, adding activated carbon to the filtrate for adsorption, adding a desorption agent to the activated carbon for desorption after the adsorption is completed, obtaining quinic acid extract, concentrating the quinic acid extract, and freeze-drying the concentrated quinic acid extract to obtain quinic acid, the extraction device of the present application has high degree of automation and can improve the crushing effect of the cranberry.

The invention belongs to the field of extraction and separation of organic acids, and relates to a method for extracting shikimic acid and quinic acid in ginkgo leaves, which overcomes the problems of long time consumption, large amount of wastewater treatment, environmental pollution, cumbersome operation and the like in the existing methods. The chemical properties of the components in Ginkgo biloba are combined with carbon dioxide supercritical extraction-extraction-membrane filtration-electrodialysis-crystallization methods, and strive to maximize the development of various active components in Ginkgo biloba on the premise of commercial value. In addition to shikimic acid and quinic acid, it also includes chlorophyll, ginkgo flavonoids, ginkgolides, ginkgo polysaccharides, etc., and the shikimic acid and quinic acid products are finally separated, and the contents are respectively greater than 98%.

The invention provides a coconut milk product capable of improving emulsion stability and a preparation process of the coconut milk product. The coconut milk product is a mechanism for improving emulsion stability through interaction between coffee polyphenol (CP) and main protein coconut globulin (CG) in coconut milk. Caffeic acid (CA), chlorogenic acid (CHA) and ferulic acid (FA) are selected as CP models. The result shows that the hydrogen bond interaction mainly occurs between the CGs and the CPs (Cl-FAslt; cG-CGlt, CG-CGlt; cG-CHA) is used in the method. CHA containing quinic acid preferentially forms a strong interaction with CG. The interaction changes the lipophilicity of the CG and promotes the formation of a compact and thick interfacial film on an oil-water interface. Furthermore, after storage, centrifugation, pH and salt treatment, the CG-CPs stable emulsion shows good stability, especially CG-CHA. The method can provide a theoretical basis for improving the stability of the coconut milk product.

Provided is an oral composition in which sourness of chlorogenic acid and quinic acid is suppressed. The oral composition contains the following components (A), (B), and (C): (A) 0.01% by mass or more and 50% by mass or less of chlorogenic acid, (B) quinic acid, and (C) isovaleric acid, wherein the mass ratio [(B) / (A)] between the component (A) and the component (B) is 0.01 or more and 0.1 or less.

The invention relates to the field of traditional Chinese medicine detection, in particular to a method for detecting chemical components of eight-component kidney-tonifying capsules. Through UPLC-Q-TOF-MS detection, 229 compounds in vitro of the eight-component kidney-tonifying capsule are characterized, 4 alkaloids, 4 quinic acids, 3 sterone, 3 anthraquinones, 3 oligosaccharides and 21 other compounds in the eight-component kidney-tonifying capsule are preliminarily classified and qualitatively determined by adopting the method, and 56 peaks are compared with a standard substance. The detection method provided by the invention can perform qualitative analysis on chemical components of the eight-seed kidney-tonifying capsule, and has guiding significance for subsequent research on in-vivo metabolism, content determination and anti-osteoporosis biological activity evaluation of the eight-seed kidney-tonifying capsule.

The present invention relates to the use of natural protectants comprising a mastic extract and optionally a second component to prevent microbial growth and preserve a product. The invention also relates to the use of natural protectants comprising quinic acid or a salt thereof, ethyl lauroyl arginate or a salt thereof, and optionally a third component to prevent microbial growth and preserve a product.

The present invention provides a method for simultaneously determining the contents of 8 components in Xiongju Shangqing Tablets. The HPLC method is used in the present invention to determine neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, luteoloside, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid. The HPLC method is adopted in the present invention to establish a method for simultaneously determining the contents of 8 components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, luteoloside, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid in Xiongju Shangqing Tablets. The method is simple and highly specific, providing a basis for the establishment of the quality standard of Xiongju Shangqing Tablets. The established method can play a role in process control to a certain extent and provide a reference for the quality control and quality evaluation of the product.

The invention provides application of a TaHQT1 gene in regulating and controlling the content of chlorogenic acid in plants, and belongs to the technical field of gene engineering. The invention provides an application of a TaHQT1 gene in regulating and controlling the content of chlorogenic acid in a plant. The invention also proves that a recombinant protein coded by the TaHQT1 gene can generate a product chlorogenic acid by taking caffeoyl-coenzyme A and quinic acid as substrates; meanwhile, overexpression is carried out on the TaHQT1 gene, and it is found that the content of chlorogenic acid in mature grains and leaves is remarkably increased, so that the TaHQT1 gene can be developed and utilized, and the content of wheat chlorogenic acid is increased through overexpression of the TaHQT1 gene, so that the nutritional quality of wheat is improved.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The invention relates to a quinic acid salicin ester derivative extracted from shiny feet, a method and application. The quinic acid salicin ester derivative is at least one of a compound 1 and a compound 2. The compound 1 and the compound 2 can be used for preparing medicines or products for preventing or treating myocardial hypertrophy diseases; and when the concentration of the compound 1 and the compound 2 is less than 50 [mu] mol / L, the compound 1 and the compound 2 have no obvious toxicity to cells and show higher biological safety. The invention provides a new choice for development of a cardiac hypertrophy inhibitor.

To provide a flavor improving agent capable of improving the undesirable flavor of food and drink containing linalool. [Solution] A flavor improver for linalool-containing food and drink, containing quinic acid as an active ingredient. It is also preferable that the amount (a) of quinic acid added to the linalool-containing food and drink is used so that the mass ratio (a / b) of the linalool content (b) in the linalool-containing food and drink is 0.00002 to 180. It is also preferable that the quinic acid content in the food and drink is 0.001 to 9 ppm.

The invention relates to a pharmaceutical composition containing caffeoyl quinic acid compounds and application of the pharmaceutical composition in treatment of rheumatoid arthritis, and the pharmaceutical composition contains active ingredients: 3-O-caffeoyl quinic acid (3-CQA) and 1, 5-O-dicaffeoyl quinic acid (1, 5-DCQA). The caffeoyl quinic acid compound is further researched, and it is surprisingly found that 3-CQA and 1, 5-DCQA are combined according to a specific proportion range, so that the treatment effect of the caffeoyl quinic acid compound on rheumatoid arthritis is further improved.

The invention relates to a polyolefin adhesive for stainless steel and a preparation method thereof, and belongs to the technical field of adhesives. According to the invention, quinic acid and shikimic acid are firstly used for modifying sodium carbonate, then modified calcium carbonate and ramie fiber are used as fillers for filling so as to construct an efficient stress transfer system, and meanwhile, maleic anhydride grafted polyethylene is also added for assisting filling of the fillers, so that the impact resistance of the prepared adhesive is effectively improved; besides, malonaldehyde is added to reduce the activity of molecules in the material and limit molecular motion, vinyl neodecanoate with a side group is doped to increase the steric hindrance of molecular motion in the system, and hexanediol diglycidyl ether is doped to increase the density of a cross-linked network of the system, so that the composite material has excellent mechanical properties. The adhesive prepared by the method has relatively high peel strength.

Chlorogenic acid or compositions containing chlorogenic acid are used in preparation of a medicament for treating sarcoma. The chlorogenic acid composition includes chlorogenic acid and coumaroyl quinic acid, can effectively treat sarcoma, and has a better therapeutic effect than the chemotherapeutic medicament doxorubicin. The experimental results show that the combination of chlorogenic acid and coumaroylquinic acid can have a synergistic effect.

The invention discloses a new method for preparing quinic acid, which specifically uses hook cone as raw material, extracts with polar solvent as solvent, enzymatically hydrolyzes with tannase, and finally passes through a polystyrene macroporous adsorption resin column Obtained by chromatography; wherein, the polar solvent is a composition of water and one or two or more selected from methanol, ethanol and acetone, and the proportion of the water is ≥ 20v / v%; the The model of the polystyrene type macroporous adsorption resin is D101, Diaion HP-20 or AB-8; The eluent used during chromatography is water and one or more than two selected from methanol, ethanol and acetone The composition, the proportion of the water is ≥ 20v / v%. In the present invention, for the first time, quinic acid with stable quality, high yield and high purity is obtained by extracting, hydrolyzing with tannin enzyme, and high-purity quinic acid by using the leaves of the quinic acid as a raw material, which not only provides a new supply raw material for the preparation of quinic acid, It also opens up a new utilization path for the hook cone.

The application relates to a new pesticide-degrading strain, in particular to a strain New bacteria Acinetobacter oryzae F9-1 . A strain F9-1 is obtained by separation, screening and identification from a soil sample in Hami City of Xinjiang Uygur Autonomous Region New bacteria Acinetobacter oryzae F9- 1 The strain is subjected to physiological and biochemical characteristic detection, the strain F9-1 shows small colonies, smooth surface, white semi-transparency on NB plates, and the results of gram staining, contact enzyme and indole experiment are positive, and the results of oxidase, V.P test and urease are negative. The strain F9-1 can hydrolyze hydrogen peroxide, can utilize L-alanine, lactic acid, L-aspartic acid and quinine acid, is sensitive to 8% NaCl, lincomycin, naphthylidine ketone acid and chloromycetin, and can degrade fluazifop-butyl and pendimethalin, and has the advantages of environmental protection, high efficiency, economy and sustainability, and is an effective means for solving the problem of pesticide pollution.

The invention discloses a method for qualitative analysis of hydroxybenzoylquinic acid and hydroxycinnamoylquinic acid isomers, and belongs to the field of separation and analytical chemistry. Monosubstituted and disubstituted hydroxybenzoylquinic acid different position isomers and hydroxycinnamoylquinic acid different position isomers are synthesized through synthesis reaction, and then the secondary mass spectrometry fragmentation law and retention time law are studied, and a method for distinguishing position isomers is provided, thereby providing a new idea for qualitative analysis of isomers without standard samples.

A powdered green tea extract composition comprising the following components (A) to (C): (A) non-polymer catechins, (B) quinic acid, and (C) high molecular weight sugar, wherein the volatile matter is 5.6% by mass or less, the mass ratio of component (A) to component (B) [(B) / (A)] is less than 0.2, and the mass ratio of component (A) to component (C) [(C) / (A)] is 1.2 or more, and the L value of the powdered green tea extract composition after being stored in an atmosphere of 37°C / 50%RH for 6 weeks is calculated. * a * b * a in the color system * The value is similar to the L value of the powdered green tea extract composition just after production. * a * b * a in the color system * The absolute value of the difference between the values ​​(Δa * ), the Δa of the powdered green tea extract composition * The value of is lower than Δa of a powdered green tea extract composition α having the same mass ratio [(B) / (A)] as the powdered green tea extract composition and not containing component (C) * , and the rate of change calculated by the following formula (2) is 5% or more. (Δa * -Δa1 * ) / Δa * ×100(2)[where Δa1 * and Δa * Compared with Δa1 described in this manual * and Δa * have the same meaning].

The invention discloses a method for inhibiting browning and microbial growth of fresh-cut potatoes, and belongs to the technical field of fresh-cut potato preservation. It is verified that quinic acid can effectively inhibit browning of the potato slurry, the fresh-cut potato shreds and the potato chips, the original color and flavor are kept, and the total number of bacterial colonies of the fresh-cut potatoes is inhibited. The use method is simple, and only a small amount of quinic acid needs to be directly added into the potato slurry, or the peeled and cut fresh-cut potatoes are soaked in a quinic acid aqueous solution. Experiments show that the compounding of quinic acid and aconitic acid can significantly inhibit browning of the fresh-cut potatoes; however, the browning of peaches, pears and apples cannot be obviously inhibited when applied to peaches, pears and apples; metabolite of the potatoes treated by quinic acid is obviously changed, and 3-methylthiopropionate and nicotinamide ribose obviously inhibit browning of the potatoes. The method is safe, free of toxic and side effects, simple in treatment, easy to operate and popularize and suitable for large-scale production and use.