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Method for identifying Polygonum bistorta L., Polygonum viviparnm L. and Polygonum paleaceum Wall.

A technology of Polygonum vulgaris and Dracula japonica, which is applied in the field of medicinal material identification, can solve the problems of insignificant differences, too strict sample size requirements, and unsatisfactory effects, and achieves the effects of simple and easy operation, guaranteed accuracy, and short analysis time.

Active Publication Date: 2019-08-02
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
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Problems solved by technology

[0003] Some literatures have used microscopic cross-section observation method to distinguish the three, and the difference is not significant; some literatures have used the clustering method to analyze the HPLC fingerprints of the water-soluble components of the three, which can be properly distinguished, but for the sample size The requirements are too strict; some literatures have used HPLC-MS-MS method to analyze the water-soluble components of the three, and the main components are basically the same, with only a small amount of difference
All methods are ineffective
[0004] According to the current "Chinese Pharmacopoeia" 2015 edition one, the traits, microscopic identification, TLC identification of gallic acid and content determination by HPLC method cannot effectively distinguish the three, resulting in Polygonum viburnum and D. The use of boxing ginseng is relatively common
The reason for this kind of problem is mainly that the specificity of the above-mentioned inspection items is not strong.

Method used

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  • Method for identifying Polygonum bistorta L., Polygonum viviparnm L. and Polygonum paleaceum Wall.
  • Method for identifying Polygonum bistorta L., Polygonum viviparnm L. and Polygonum paleaceum Wall.
  • Method for identifying Polygonum bistorta L., Polygonum viviparnm L. and Polygonum paleaceum Wall.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Instruments and reagents

[0032] Instrument: Mettler Toledo XSE205 electronic balance;

[0033] Waters 2695-2998 high performance liquid chromatography (PDA detector);

[0034] Reference substance: chlorogenic acid, purchased from China National Institutes for Food and Drug Control; batch number 110753-201314, content 96.6%; 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid Nitric acid, all purchased from Chengdu Pusi Biotechnology Co., Ltd., the batch numbers are ps100623-02 and ps100623-01, respectively, and the purity is ≥98%;

[0035] Reagent: Methanol and acetonitrile are chromatographically pure, and phosphoric acid is analytically pure. There are 10 batches each of boxing ginseng medicinal materials, Polygonum versicolor medicinal materials, and grass blood root medicinal materials, all of which are collected from the medicinal material market.

[0036] 2. Solution Preparation

[0037] 2.1 Preparation of mixed reference substance solution: Accurate...

Embodiment 2

[0047] Embodiment 2 accuracy test

[0048] 1. Identification of samples by gene sequencing

[0049] Genomic DNA was extracted from the collected 30 batches of ginseng, Polygonum japonicus, and Dracaena japonicus using kits, the ITS2 sequence was amplified by PCR, sequenced, and the sequencing results were spliced ​​with Codencode software, and compared and analyzed on Genbank. The results are shown in Table 1.

[0050] The sequencing results of the 30 batches of samples collected in Table 1

[0051] Sample serial number Number / Accession No. species sequence similarity 1 KX167974.1 Polygonum bistorta L. (Boxing ginseng) 100.00％ 2 KX167974.1 Polygonum bistorta L. (Boxing ginseng) 100.00％ 3 KX167974.1 Polygonum bistorta L. (Boxing ginseng) 100.00％ 4 KX167974.1 Polygonum bistorta L. (Boxing ginseng) 100.00％ 5 KX167974.1 Polygonum bistorta L. (Boxing ginseng) 100.00％ 6 KX167974.1 Polygonum bistorta L. (Boxing gin...

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Abstract

The invention relates to the technical field of identification of medicinal materials and especially relates to a method for identifying Polygonum bistorta L., Polygonum viviparnm L. and Polygonum paleaceum Wall.. The method takes three components, that is, chlorogenic acid, 3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid, as distinguishing substances. The method is simple and easy tooperate, short in analysis time and high in accuracy reaching 100%, and is suitable for being widely promoted.

Description

technical field [0001] The invention relates to the technical field of identification of medicinal materials, in particular to a method for identification of Polygonum japonicum and Rhizoma praecox. Background technique [0002] Ginseng is the dry rhizome of Polygonum bistorta L., a plant of the Polygonaceae family. It is oblate strip or flat cylinder, curved, some are curved in opposite directions, both ends are slightly pointed, or one end is tapered, 6-13cm long, 1-2.5cm in diameter. The surface is purple-brown or purple-black, rough, one side is raised, the other side is slightly flat or slightly grooved, the whole body is densely ringed, and there are residual fibrous roots or root marks. Hard, light brown-red or brown-red cross-section, vascular bundles are yellow-white dot-shaped, arranged in rings. Gas micro, bitter, astringent. Polygonum viviparnm is the dry rhizome of Polygonum viviparnm L. Dried grass blood is the dry rhizome of Polygonum paleaceum Wall. The ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 林林林永强于凤蕊穆向荣解盈盈
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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