Rapid test strip for chloramphenicol drug residue

A technology for detecting test strips and chloramphenicol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, difficult to popularize, expensive equipment, etc. easy effect

Inactive Publication Date: 2019-05-28
ZHENJIANG YITE BIOTECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing technologies are mainly gas chromatography-mass spectrometry, high performance liquid chromatography and other detection methods for the detection of chloramphenicol, but the defects of these technologies are obvious: expensive equipment, complicated operation, difficult to popularize

Method used

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  • Rapid test strip for chloramphenicol drug residue
  • Rapid test strip for chloramphenicol drug residue
  • Rapid test strip for chloramphenicol drug residue

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Embodiment Construction

[0018] To prepare test strips for rapid detection of chloramphenicol residues, it is first necessary to prepare the carrier protein and gold-labeled antibody coupled to chloramphenicol drug, so as to prepare the detection blot and gold-labeled antibody fiber; secondly, it is necessary to prepare goat anti or rabbit anti-mouse IgG Antibodies, used to prepare control blots.

[0019] 1. Conjugation of Chloramphenicol Drug to Carrier Protein

[0020] Coupling of chloramphenicol with ovalbumin and hemocyanin by mixed anhydride method and carbodiimide method respectively to obtain coating agent (for immobilization on reaction membrane) and immunogen (for preparation of monoclonal antibody) .

[0021] (1) Preparation of the original coating:

[0022] ①Dissolve 5mg of chloramphenicol in 0.5mL formamide (DMF), cool to 10°C, add 5 μl of isobutyl chloroformate, and stir for 30 minutes at 4°C to obtain the reaction solution Ⅰ;

[0023] ② Weigh 30 mg of ovalbumin (OVA), and dissolve it ...

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Abstract

The invention discloses a test strip and belongs to the field of rapid food safety detection. The test strip comprises a base plate, a sample pad, a conjugate release pad, a reaction film and a water-absorbing pad, wherein the sample pad, the conjugate release pad, the reaction film and the water-absorbing pad are attached to the base plate and tightly connected in sequence, and a part of the conjugate release pad covers the lower portion of the sample absorbing pad. The reaction film is coated with a detection line composed of a chloramphenicol-carrier protein conjugate and a quality controlline coated with goat anti-mouse IgG; the conjugate release pad is coated with a colloidal gold label of chloramphenicol. The test strip can prolong the observation time of a test result, the sample absorbing pad can also fully absorb a detection liquid to make the detection liquid completely be in contact with a gold-labeled antibody for a full reaction, then chromatography is carried out to achieve a reaction on the reaction film, and the errors can be effectively reduced. The situation can also be prevented that the protein in the gold-labeled antibody is inactivated by some interference components in a test sample, and the combination of the gold-labeled antibody and a coating antigen is influenced.

Description

technical field [0001] The invention relates to a rapid detection test strip for detecting chloramphenicol drug residues, belonging to the field of rapid detection of food safety. technical background [0002] Chloramphenicol is a broad-spectrum antibiotic, commonly used in the treatment of various infectious diseases in animals, and has a strong inhibitory effect on a variety of pathogenic bacteria. Chloramphenicol has serious side effects, which can inhibit the hematopoietic function of human bone marrow and cause human aplastic anemia and other diseases. Therefore, chloramphenicol residues in animal food pose a huge threat to human health. [0003] At present, the determination methods of chloramphenicol and its metabolites in food of animal origin are mainly physical and chemical detection methods. For example, chemical analysis is used to determine the residual amount of chloramphenicol in shrimp meat, and the detection limit is 0.0125ug / kg. Determination of chloramphe...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/577G01N33/544
Inventor 洪霞立雯馨秦悦
Owner ZHENJIANG YITE BIOTECH DEV CO LTD
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