Rapid test strip for chloramphenicol drug residue
A technology for detecting test strips and chloramphenicol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, difficult to popularize, expensive equipment, etc. easy effect
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[0018] To prepare test strips for rapid detection of chloramphenicol residues, it is first necessary to prepare the carrier protein and gold-labeled antibody coupled to chloramphenicol drug, so as to prepare the detection blot and gold-labeled antibody fiber; secondly, it is necessary to prepare goat anti or rabbit anti-mouse IgG Antibodies, used to prepare control blots.
[0019] 1. Conjugation of Chloramphenicol Drug to Carrier Protein
[0020] Coupling of chloramphenicol with ovalbumin and hemocyanin by mixed anhydride method and carbodiimide method respectively to obtain coating agent (for immobilization on reaction membrane) and immunogen (for preparation of monoclonal antibody) .
[0021] (1) Preparation of the original coating:
[0022] ①Dissolve 5mg of chloramphenicol in 0.5mL formamide (DMF), cool to 10°C, add 5 μl of isobutyl chloroformate, and stir for 30 minutes at 4°C to obtain the reaction solution Ⅰ;
[0023] ② Weigh 30 mg of ovalbumin (OVA), and dissolve it ...
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