Cell bionic intelligent production system

A production system and cell technology, applied in the field of cell bionic intelligent production system, can solve the problems of adverse effects, low red blood cell denucleation efficiency, etc., and achieve the effect of in vitro scale and denucleation

Active Publication Date: 2020-07-10
赵涌
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are studies that can continuously expand hematopoietic stem cells in vitro for 45 days in a serum-free culture system, and the number of cells can reach 10 7 times, but the red blood cell denuclearization efficiency is low, which cannot meet the requirements
Although the complete denucleation of erythroid progenitor cells is achieved by the hematopoietic support of stromal cells, these stromal cells have adverse effects on later blood transfusions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell bionic intelligent production system
  • Cell bionic intelligent production system
  • Cell bionic intelligent production system

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] The hollow fiber tubes of the present invention can be produced by known methods. In an exemplary method, the method of making the hollow fiber tube of the present invention comprises the following steps:

[0043] (1') Steps for preparing activated hollow fibers

[0044] Activated hollow fibers can be prepared by methods known in the art. In certain embodiments, activated hollow fibers are prepared from chloromethyl ether-modified polysulfone by, for example, melt extrusion.

[0045] In an exemplary method, the melt extrusion of polysulfone modified with chloromethyl ether is carried out by the melt extrusion method for producing plastic products, gradually cooled in the atmosphere, and the melt fluidity gradually increases with the decrease of the temperature of the melt. Reduced and solidified into shape.

[0046] In another exemplary method, an activated hollow fiber with an asymmetric structure is prepared by, for example, a profiled spinneret forming method. Sp...

Embodiment 1

[0072] This embodiment is the preparation of the hollow fiber tube 1 .

[0073] Soak 0.2kg of polysulfone hollow fiber C2011 (FiberCell Systems, Frederick, MD) in 1000ml of organic solvent composed of chloromethyl ether and dioxyethane with a volume ratio of 1:10, add 8g of chlorinated zinc. The polysulfone hollow fiber was reacted at 40°C for 12 hours. During the reaction, the organic solvent is intermittently stirred to keep the uniformity of the reaction between the hollow fiber tube and the organic solvent. The activated hollow fiber tubes were then rinsed several times with deionized water. It was then dried at 60°C to obtain activated hollow fibers. The activated hollow fiber was soaked in 25% TMA solution at room temperature for 45 hours to obtain the hollow fiber tube 1 of the present invention.

Embodiment 2

[0075] This embodiment is the preparation of the hollow fiber tube 2 .

[0076] Dissolve the polysulfone modified by chloromethyl ether in DMF organic solvent, use PEG6000 as an additive, undergo miscible degassing, use a metering pump (specification 1.2ml / r), insert tube spinneret, nitrogen cylinder, etc. A hollow fiber membrane spinning machine is used to prepare hollow fibers, and after air exposure, a coagulation bath (gel bath temperature 25° C., solvent concentration 55% in the gel bath, and 78% solvent concentration in the core liquid) is carried out. Activated hollow fiber tubes with an asymmetric structure were thus prepared. The activated hollow fiber was soaked in 25% TMA solution for 45 hours at room temperature to obtain the hollow fiber tube 2 of the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
thicknessaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

Disclosed in the present invention is a cell bionic intelligent production system, comprising a bioreactor, a medium container, and an oxygen supplier. The bioreactor comprises a sealed chamber formed by a housing and multiple hollow fiber tubes disposed in the chamber; the housing is provided with a medium inlet, a medium outlet, and an oxygen inlet; the inner cavity of the hollow fiber tube allows a basal medium and / or a gas to circulate, and the tail end of the hollow fiber tube can be communicated with a basal medium container and / or the oxygen supplier; the wall of the hollow fiber tube allows a component of the basal medium and / or a gas to pass from the inner cavity side of the hollow fiber tube to the outside through the wall; at least the outer surface of the wall of the hollow fiber tube is provided with a group suitable for cell denucleation. The medium container is provided with a supply port, and the supply port is connected to the medium inlet by means of a pipeline; the oxygen supplier is connected to the oxygen inlet by means of a pipeline. The cell bionic intelligent production system provided by the present invention is particularly suitable for cultivation of erythroid progenitor cells.

Description

technical field [0001] The invention relates to a cell production system, in particular to a cell bionic intelligent production system. Background technique [0002] According to data from the World Health Organization (WHO) global blood safety database, 92 million units of whole blood donations are collected globally every year, 3.3% of hospitals postpone operations every day due to blood shortages, and 10.3% of hospitals have at least 1 day of emergency operations every year. Blood is available. In addition, 5-10% of blood transfusions are unsafe due to reasons such as HIV infection. Therefore, the need for blood supply is very urgent on a global scale. [0003] With the in-depth research on the differentiation and development of hematopoietic stem cells, artificially inducing the expansion of hematopoietic stem progenitor cells to produce mature red blood cells has gradually become a potential and feasible direction to solve the difficulties faced by blood transfusion. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): C12M3/00C12M1/24C12M1/04C12M1/00
CPCA61M1/34C12M1/00C12M1/04C12M1/24C12M3/00
Inventor赵涌
Owner赵涌