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A cross-linking treatment method for improving the stability of biological valve elastin

An elastin and cross-linking treatment technology, which is applied in the field of biomedical materials and medical devices, can solve problems such as no good methods, and achieve the effects of prolonging service life, increasing elastin content and anti-calcification performance

Active Publication Date: 2019-11-12
VENUS MEDTECH (HANGZHOU) INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Therefore, by optimizing the chemical cross-linking method of biological heart valves, especially the development of new material processing methods that can increase elastin content and structural stability, the overall structural stability and anti-calcification performance of biological heart valves will be improved. The development of related industries is of great significance, but there is no good method at present, so it needs to be improved

Method used

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  • A cross-linking treatment method for improving the stability of biological valve elastin
  • A cross-linking treatment method for improving the stability of biological valve elastin
  • A cross-linking treatment method for improving the stability of biological valve elastin

Examples

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Embodiment 1

[0024] This example provides a cross-linking treatment method for improving the stability of biological valve elastin. In all embodiments, fresh pig pericardium comes from a local slaughterhouse.

[0025] Such as figure 1 As shown, the freshly collected porcine pericardium was washed in distilled water for 2 hours at 4 degrees Celsius and 100 RPM under shaking conditions, and then soaked in 0.625% glutaraldehyde for 24 hours, and then the pericardium was soaked in tropoelastin and lysyl oxidation. In the enzyme aqueous solution for 24 hours, the mass feed ratio is 0.01mg proelastin: 1mg pericardium and 1ug lysyl oxidase: 1mg pericardium. Finally, the pericardium was soaked in 1% epigallocatechin gallate aqueous solution for 24 hours.

[0026] Such as figure 2 As shown, it is a schematic diagram of the principle of using tropoelastin and lysyl oxidase cross-linking and tea polyphenol combined treatment to improve the content and stability of pericardium elastin.

[0027] Du...

Embodiment 2

[0029] This example provides a cross-linking treatment method for improving the stability of biological valve elastin. In all embodiments, fresh pig pericardium comes from a local slaughterhouse.

[0030] Such as figure 1 As shown, the freshly collected porcine pericardium was washed in distilled water for 2 hours at 4 degrees Celsius and 100 RPM under shaking conditions, and then soaked in 0.625% glutaraldehyde for 24 hours, and then the pericardium was soaked in tropoelastin and lysyl oxidation. In the enzyme aqueous solution for 24 hours, the mass feed ratio is 0.05mg proelastin: 1mg pericardium and 1ug lysyl oxidase: 1mg pericardium. Finally, the pericardium was soaked in 1% epigallocatechin gallate aqueous solution for 24 hours.

[0031] Such as figure 2 As shown, it is a schematic diagram of the principle of using tropoelastin and lysyl oxidase cross-linking and tea polyphenol combined treatment to improve the content and stability of pericardium elastin.

Embodiment 3

[0033] This example provides a cross-linking treatment method for improving the stability of biological valve elastin. In all embodiments, fresh pig pericardium comes from a local slaughterhouse.

[0034] Such as figure 1 As shown, the freshly collected porcine pericardium was washed in distilled water for 2 hours at 4 degrees Celsius and 100 RPM under shaking conditions, and then soaked in 0.625% glutaraldehyde for 24 hours, and then the pericardium was soaked in tropoelastin and lysyl oxidation. In the enzyme aqueous solution for 24 hours, the mass feed ratio is 0.25mg proelastin: 1mg pericardium and 1ug lysyl oxidase: 1mg pericardium. Finally, the pericardium was soaked in 1% epigallocatechin gallate aqueous solution for 24 hours.

[0035] Such as figure 2 As shown, it is a schematic diagram of the principle of using tropoelastin and lysyl oxidase cross-linking and tea polyphenol combined treatment to improve the content and stability of pericardium elastin.

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Abstract

The invention discloses a cross-linking treatment method for improving stability of biovalve elastin. The cross-linking treatment method comprises the steps of soaking glutaraldehyde cross-linked pericardium in a tropoelastin and lysyl oxidase aqueous solution so as to realize original position cross-linking of elastin on pericardium, and then stabilizing the elastin with tea polyphenols. The method provided by the invention can improve the content of elastin in biomaterials, and the calcification resistance, and the service life is potentially prolonged.

Description

technical field [0001] The invention relates to the technical field of biomedical materials and medical devices, in particular to a cross-linking treatment method for improving the stability of biological valve elastin. Background technique [0002] Heart valve disease is a common disease of valve degeneration. Anatomically, it manifests as narrowing of the blood passage or valvular insufficiency. [0003] Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. [0004] Biological heart valves refer to a class of biomedical materials used to replace human diseased heart valves. Biological heart valves are ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 雷洋王云兵
Owner VENUS MEDTECH (HANGZHOU) INC
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