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Composition for catalyzing degradation of uric acid in intestinal tracts, as well as preparation method and application thereof

A composition, uric acid technology, applied in the field of uric acid or urate degradation, to achieve the effect of reducing the incidence of disease, reducing the content of uric acid, and improving the activity of urate oxidase

Active Publication Date: 2019-06-07
WUHAN KANGFUDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yin Yanyan and others studied the uric acid-lowering effect of Cordyceps militaris on patients with hyperuricemia, and found that it has a uric acid-lowering effect, but the way of taking the material is heating and boiling, so it can be concluded that the effective substance is a heat-resistant material, not uric acid oxidation. enzyme

Method used

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  • Composition for catalyzing degradation of uric acid in intestinal tracts, as well as preparation method and application thereof
  • Composition for catalyzing degradation of uric acid in intestinal tracts, as well as preparation method and application thereof
  • Composition for catalyzing degradation of uric acid in intestinal tracts, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Isolation and preservation of Cordyceps militaris (Cordyceps militaris)

[0031] The researcher of the present invention buys fresh Cordyceps militaris from Wuhan farmer's market, adopts tissue separation method to carry out the separation and purification of Cordyceps militaris. The specific operation is as follows: clean the surface of fresh Cordyceps militaris fruiting body with sterile water, put it into a sterile petri dish in the ultra-clean workbench, clamp 75% (v / v) alcohol cotton balls on Cordyceps militaris with sterilized tweezers The surface of the fruiting body is disinfected, the fruiting body is cut open with a sterilized scalpel, and the bacterial meat tissue pieces of about 2*2mm inside the fruiting body are cut, moved to sterilized PDA slant medium (200g potato, 10g glucose , 15g of agarose, distilled to 1L with pure water, sterilized at 121°C), placed in a constant temperature incubator at 26°C for cultivation. Check the colony growth e...

Embodiment 2

[0032] Embodiment 2: the preparation method of the Cordyceps militaris fungus powder containing uric acid oxidase

[0033] The Cordyceps militaris (Cordyceps militaris) preserved in Example 1 on the PDA solid medium was picked and transferred to the fungal culture medium plate (peptone 0.5wt%, yeast extract powder 0.2wt%, glucose 2wt%, dihydrogen phosphate Potassium 0.1wt%, magnesium sulfate 0.05wt%, 2.0wt% agarose, pure water to make up to a constant volume, pH 6.2~6.6), culture at 25-28°C for 7-10 days for activation culture, cut 1cm×1cm from the plate The bacterial block, after chopping, is inoculated to the seed medium (sucrose 2.0wt%, peptone 1.0wt%, yeast extract 1.0wt%, dipotassium hydrogen phosphate 0.5wt%, sodium dihydrogen phosphate 1.0wt%, pure water constant volume Filling, pH6.5), placed at 150rpm, cultured at 25-28°C for 3-5 days, then crushed the bacterial block with a sterile hand-held homogenizer, continued to cultivate under the same conditions for 3-5 days, ...

Embodiment 3

[0034] Embodiment 3: the preparation method of the Cordyceps militaris fungus powder containing uric acid oxidase

[0035] The Cordyceps militaris (Cordyceps militaris) preserved in Example 1 on the PDA solid medium was picked and transferred to the fungal culture medium plate (peptone 0.5wt%, yeast extract powder 0.2wt%, glucose 2wt%, dihydrogen phosphate Potassium 0.1wt%, magnesium sulfate 0.05wt%, 2.0wt% agarose, pure water to fill up, pH 6.2 ~ 6.6), 25-28 ° C for 6-7 days for activation culture, cut from the plate 1cm × 1cm The bacterium block, after chopping, is inoculated to the seed culture medium (sucrose 2.0wt%, peptone 1.0wt%, yeast extract 1.0wt%, dipotassium hydrogen phosphate 1.0wt%, sodium dihydrogen phosphate 1.0wt%, pure water constant volume Filling, pH6.5), placed at 150rpm, cultured at 25-28°C for 3-5 days, then crushed the bacterial block with a sterile hand-held homogenizer, continued to cultivate under the same conditions for 3-5 days, and then used A st...

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Abstract

The invention relates to a composition for catalyzing degradation of uric acid in intestinal tracts. The composition comprises cordyceps militaris powder or dry enzyme powder, which contains urate oxidase which can be used for catalyzing degradation of uric acid. The invention further provides a preparation method and application of the composition for catalyzing degradation of uric acid in intestinal tracts. According to the composition, uric acid is catalyzed to be degraded into allantoin, the content of uric acid in intestinal tracts can be reduced, the proportion of uric acid, secreted andexcreted to the intestinal tracts, in blood can be increased, the concentration of uric acid in the blood can be reduced, and hyperuricemia and gout diseases can be prevented and treated; and excretion of uric acid in urea can be reduced, and the incidence rate of uric acid calculus can be reduced.

Description

technical field [0001] The invention relates to the field of degradation of uric acid or urate in human or animal intestines, in particular to a composition capable of catalyzing the degradation of uric acid in intestinal tracts and its preparation method and application. Background technique [0002] Hyperuricemia is a chronic metabolic disease, which is manifested by blood uric acid levels higher than the normal range (male blood uric acid concentration greater than 420 μmol / L, female blood uric acid concentration greater than 360 μmol / L). In addition to gout caused by inflammation caused by crystalline uric acid, patients with hyperuricemia may also develop kidney disease, urinary stones, cardiovascular disorders, and cerebrovascular disorders. [0003] Gout is a complication of hyperuricemia, a disease with elevated blood uric acid as the main biochemical indicator, and is closely related to diet structure and lifestyle. With the improvement of domestic living standards...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61K38/44A61P19/06A61P13/04A61P13/12A23L33/00A23L31/00
Inventor 刘海峰刘艳红陈火晴刘红黄荷
Owner WUHAN KANGFUDE BIOTECH CO LTD
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