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Tubular scleroderma guani counting method and application thereof

A tube-type swollen-legged bee and counting method technology, which is applied in the field of image recognition, achieves the effect of small external interference and large error in solving time-consuming

Pending Publication Date: 2019-06-07
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The counting method of the present invention obtains the number of tube-type swollen-legged wasps by freezing the tube-type swollen-legged wasps, sieving, taking pictures, and counting by ImageJ software, thereby overcoming the defects of manual counting

Method used

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  • Tubular scleroderma guani counting method and application thereof
  • Tubular scleroderma guani counting method and application thereof
  • Tubular scleroderma guani counting method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Basic Mode

[0049] The specific tube counting method for Strophaga is:

[0050] (1) Place the tube containing the tube-type Sclerophyllum sample at -20°C for 24h-48h to ensure that it does not move during subsequent operations;

[0051] (2) Take out the frozen tube scorpion bee, peel off the bee and the feed with tweezers, and discard the large pieces of feed impurities;

[0052] (3) Put the tube-type scorpion bee on a sieve of 40 meshes, and shake it lightly. Small impurities leak under the sieve to remove small impurities, and the tube-type scorpion bee is basically left on the sieve;

[0053] (4) Filter paper (Length*Width=30cm*30cm) Adhere the filter paper to the table with double-sided tape, and it needs to be completely flat; the tube-type Sclerophagus after sieving is placed on the filter paper, placed independently between individuals, intelligent Take photos with a mobile phone or camera, and the pixels of the photo are 4032*3024 or 3024*4032 (width*height), ...

Embodiment 2

[0058] Example 2: Optimization of sieve mesh

[0059] The main steps are basically the same as steps (1)-(3) in Example 1, except that the number of meshes selected in step (3) is carried out according to Table 1 below. In this example, multiple groups of S. scorpione samples to be counted were tested.

[0060]

[0061] Test, the test results are shown in Table 1. The results showed that the tubular scorpion wasp passed a 20-mesh sieve, and the number of insects under the sieve was 22-80; after a 40-mesh sieve, only one bee fell under the sieve, and the amount of impurities on the sieve was small; On the sieve, the amount of impurities on the sieve is slightly larger; after passing a 100-mesh sieve, although all the bees are on the sieve, the amount of impurities is higher. The photos are not suitable for software counting. Therefore, the mesh number of the tube-type Sclerophyllum sieve is selected to be 40, and occasionally the bees that fall under the sieve can be retrieved wit...

Embodiment 3

[0062] Example 3: Counting the same photo with different minimum sizes

[0063] (1) Place the tube containing the tube-type Sclerophyllum sample at -20°C for 24h-48h to ensure that it does not move during subsequent operations;

[0064] (2) Take out the frozen tube scorpion bee, peel off the bee and the feed with tweezers, and discard the large pieces of feed impurities;

[0065] (3) Put the tube-type scorpion bee on a sieve of 40 meshes, and shake it lightly. Small impurities leak under the sieve to remove small impurities, and the tube-type scorpion bee is basically left on the sieve;

[0066] (4) Filter paper (Length*Width=30cm*30cm) Adhere the filter paper to the table with double-sided tape, and it needs to be completely flat; the tube-type Sclerophagus after sieving is placed on the filter paper, placed independently between individuals, intelligent Take photos with a mobile phone or camera, and the pixels of the photo are 4032*3024 or 3024*4032 (width*height), but the tube-shap...

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Abstract

The invention discloses a tubular scleroderma guani counting method and application thereof. The counting method comprises the steps of freezing of the tubular scleroderma guani, impurity removal, photographing and Image J software counting. The camera is used for providing photos with appropriate pixels used for counting by Image J software. And Image J software is used for counting, and countingof the number of the tubular scleroderma harzianum openings in the photo is completed by setting relevant parameters. According to the method, the number of the bee larva mouths of the tubular scleroderma guani is counted through the method combining the photo and the software, statistics of the number of the bee larva mouths can be rapidly completed, the counting accuracy is high, and the defects that manual counting wastes time and is prone to errors are overcome.

Description

Technical field [0001] The invention belongs to the field of image recognition, and relates to a tubular scorpion bee counting method, in particular to a tubular scorpion bee counting method using software, and the application of the method in the tube-type scorpion bee production and release monitoring and bee number verification . Background technique [0002] Scleroderma guani Xiao et Wu is a parasitic natural enemy insect, belonging to the Hymenoptera Bethylidae family. The host is the larvae and pupae of borer pests such as Coleoptera. It is currently mainly used to control the harm of long beetles, especially Monochamus alternatus Hope (1842). This is because Monochamus alternatus Hope is the main vector insect of pine wood nematode disease, which is caused by pine trees. A devastating epidemic. The biological control of forestry pests and diseases is an effective means to ensure the green mountains and green waters. Therefore, the tube scorpion bee is purchased for the p...

Claims

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Application Information

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IPC IPC(8): G06T7/00G06T7/41
Inventor 王俊平张培王婷婷田小草张妍
Owner QINGDAO AGRI UNIV
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