A kind of method of extracting golden pine biflavone from ginkgo leaf

A technology of golden pine biflavonoids and ginkgo biloba leaves is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, organic chemistry, etc., which can solve the problems of high cost, insufficient extraction, etc., reduce emissions, facilitate large-scale promotion, The effect of convenient operation

Active Publication Date: 2021-01-05
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, when extracting the biflavonoids from Ginkgo biloba, the extraction is not thorough enough, the cost is high, and it cannot meet the needs of the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 cellulase enzymolysis time selection

[0020] Ginkgo biloba leaves were crushed through a 40-mesh sieve, and 100 g of the sample was added to water of the same quality to make leaf pulp, and cellulase was added. The mass ratio of cellulase and leaf pulp was 1:400, and enzymatic hydrolysis was carried out at 37-40°C for 2 hours. , 2.5h, 3h, 3.5h, 4h, inactivate the enzyme at 90°C for 5min to obtain the enzymolysis solution A, adjust the pH of the enzymolysis solution A to 4.7, add the mass ratio of hemicellulase and ligninase to 1.5-2 : 1 compound enzyme preparation, the mass ratio of the compound enzyme preparation and enzymolysis solution A is 1:400, enzymolysis at 48-50°C for 1.0h, and inactivating the enzyme at 90°C for 5min to obtain the crude extract of enzymolysis; The crude extract and an equal volume of methanol are fully mixed, extracted 2-3 times, the extracts are combined, concentrated under reduced pressure to obtain a concentrated solution; the...

Embodiment 2

[0024] Embodiment 2 hemicellulase and ligninase enzymolysis time selection

[0025] Ginkgo biloba leaves were crushed through a 40-mesh sieve, 100 g of the sample was taken and water of equal mass was added to make leaf pulp, cellulase was added, the mass ratio of cellulase and leaf pulp was 1:400, and enzymolysis was carried out at 37-40°C for 3 hours, Inactivate the enzyme at 90°C for 5 minutes to obtain the enzymatic solution A, adjust the pH of the enzymatic solution A to 4.7, add a compound enzyme preparation with a mass ratio of hemicellulase and ligninase of 1.5-2:1, the compound enzyme preparation The mass ratio of enzymolysis solution A to enzymolysis solution A is 1:400, enzymolysis at 48-50°C for 0.5h, 1.0h, 1.5h, 2h, and inactivating the enzyme at 90°C for 5min to obtain enzymolysis crude extract; enzymolysis crude extract Liquid and an equal volume of methanol are fully mixed, extracted 2-3 times, combined extracts, and concentrated under reduced pressure; The mi...

Embodiment 3

[0029] The selection of embodiment 3 eluent ratio

[0030] Crush Ginkgo biloba leaves through a 40-mesh sieve, take 100 g of the sample, add water of equal quality to make leaf pulp, add cellulase, the mass ratio of cellulase and leaf pulp is 1:400, and enzymatically hydrolyze at 37-40°C for 3 hours , inactivate the enzyme at 90°C for 5 minutes to obtain enzymolysis solution A, adjust the pH of enzymolysis solution A to 4.7, add a compound enzyme preparation with a mass ratio of hemicellulase and ligninase of 1.5-2:1, the compound enzyme The mass ratio of preparation and enzymolysis solution A is 1:400, enzymolysis at 48-50°C for 1.5h, inactivation of enzyme at 90°C for 5min, to obtain enzymolysis crude extract; enzymolysis crude extract and an equal volume of methanol are sufficient Mix and extract 2-3 times, combine the extracts, and concentrate under reduced pressure; the obtained concentrated solution is added to a chromatographic column with silica gel as an adsorbent for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting sciadopitysin from ginkgo leaves. According to the method, the enzymatic hydrolysis is used to pretreat ginkgo leaves, multiple extractions and separations are performed on a crude extraction liquid, and the sciadopitysin extract with relatively high yield is obtained through simple process conditions. In the method, the enzymatic hydrolysis is usedfor pre-treatment to improve the extraction rate of the product, and the extraction rate is higher than 96%. The enzymatic hydrolysis can be easily operated in a large area, and the enzymatic hydrolysis reactions of enzymic preparations can reduce the use of organic solvents, so that the discharge of organic wastewater is reduced, and the production concept of green and environmental protection isconducive to achieve. The product is fully extracted, the purity of the product is high, and the content of the product is 99.5% or more, so that the action effects of sciadopitysin is improved, andthe method is conducive to the wide application of sciadopitysin.

Description

technical field [0001] The invention relates to a method for extracting aquinone from ginkgo leaves, and relates to the field of traditional Chinese medicine extraction technology. Background technique [0002] Diabetic heart disease refers to the concurrent or concomitant heart disease of diabetic patients. 70% to 80% of diabetic patients die of cardiovascular complications. Physiological, non-invasive cardiac function tests, and pathological and anatomical data are key factors in the discovery of macrovascular lesions and microvascular lesions. Diabetic macrovascular disease is the accelerated development of atherosclerosis, which is the cause of increased myocardial infarction in diabetic patients. The famous heart prevention study (HPS) has confirmed that giving statins to strictly control blood lipids (especially LDL) patient group, the incidence of cardiovascular events was significantly lower than the poor blood lipid control group, and diabetic patients benefited mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/40C07D311/30A61K31/352
Inventor 张珊珊丁书文郭良清时吉来李运伦
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap