Connecting mechanism for collecting and detecting fluorescence

Abstract

The invention discloses a connecting mechanism for collecting and detecting fluorescence. The connecting mechanism for collecting and detecting the fluorescence comprises a fluorescence collecting device, a wavelength selection device, a fluorescence focusing device, a darkroom sleeving and a fluorescence detecting device, and the fluorescence collecting device, the wavelength selection device, the fluorescence focusing device, the darkroom sleeving and the fluorescence detecting device are sequentially connected; the fluorescence collecting device comprises a sleeving a, a lens a, a connecting tube a, the lens a is arranged in the sleeving a, and the sleeving a is connected with the connecting tube a; the wavelength selection device comprises a light filter placing frame A, a light filterplacing frame B, a placing seat and a base; the fluorescence focusing device comprises a sleeving b, a lens b, a connecting tube b, the lens b is arranged in the sleeving b, and the sleeving b is connected with the connecting tube b; the fluorescence detecting device comprises a front plate, a detector and a detector base plate, the front plate is fixed to one end of the detector, and the detector is fixed to the detector base plate. According to the connecting mechanism for collecting and detecting the fluorescence, internal coordinating of the whole device is compact, production and assembling are facilitated, the fluorescence can enter from only one side, the other places form a darkroom, and detecting accuracy is improved.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection, in particular to a connection mechanism for fluorescence collection and detection. Background technique [0002] Fluorescence detection is a commonly used detection technology, which uses the characteristics of the analyte to produce fluorescence when excited by light of a specific wavelength for qualitative and quantitative detection. This method is not only convenient and fast, but also usually has high sensitivity and selectivity. [0003] During the detection process of fluorescence lifetime, when the laser hits the sample, the fluorescence emitted by the sample diverges, and the divergent fluorescence needs to be collected; the common problems of the existing fluorescence detection devices are: the fluorescence collection efficiency is low, and the collected fluorescence cannot be collected. The wavelength of the light is selected, the focus position after the fluorescence co...

AI Technical Summary

Problems solved by technology

[0003] During the detection process of fluorescence lifetime, when the laser hits the sample, the fluorescence emitted by the sample diverges, and the divergent fluorescence needs to be collected; the common problems of the existing fluorescence detection devices are: the fluorescence collection efficiency is low, and the collected fluorescence cannot be collected. The wavelength of the light is selected, the focus position after the fluorescence converges will have a little deviation, and the background light will affect the detection result, so the detection accuracy is poor

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