Preparation method and applications of fluorescently-labeled rice bran polysaccharide iron complex

A technology of rice bran polysaccharide and fluorescent labeling is applied in the field of tracing, which can solve the problems of difficulty in tracing rice bran polysaccharide iron and the like, and achieve the effects of low detection cost, rapid method and easy promotion.

Inactive Publication Date: 2019-06-18
WUHAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The main purpose of the present invention is to propose a preparation method and application of fluorescently label

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  • Preparation method and applications of fluorescently-labeled rice bran polysaccharide iron complex
  • Preparation method and applications of fluorescently-labeled rice bran polysaccharide iron complex
  • Preparation method and applications of fluorescently-labeled rice bran polysaccharide iron complex

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preparation example Construction

[0041] In view of this, the present invention proposes a preparation method of fluorescently labeled rice bran polysaccharide iron, which uses the characteristic that the reducing end of rice bran polysaccharide iron molecule reacts with the active amino group in rhodamine B to prepare a fluorescent Tracer fluorescently labeled rice bran polysaccharide iron. Combine now figure 1 The schematic flow chart of an embodiment of the preparation method of the fluorescently labeled rice bran polysaccharide iron is described, and the preparation method of the fluorescently labeled rice bran polysaccharide iron includes the following steps:

[0042] Step S110, dissolving iron rice bran polysaccharide and rhodamine B in water, and then adding an aprotic solvent to react to form a mixed solution.

[0043] In this embodiment, the mass ratio of the rice bran polysaccharide iron and rhodamine B is preferably (10-1):1; the volume ratio of the water and the aprotic solvent is preferably 10:(1...

Embodiment 1

[0069] Add 10% sodium hydroxide solution dropwise to the rice bran polysaccharide aqueous solution until the pH is 8, and add 10% ferric chloride solution dropwise to the above rice bran polysaccharide under the condition of pH 8-9 in a water bath at 70°C React in an aqueous solution to form a reaction solution until a reddish-brown precipitate appears in the reaction solution. Centrifuge the reaction solution, add absolute ethanol 3 times the volume of the supernatant to the supernatant, let stand at 0°C for 14 hours, centrifuge the precipitate, wash the precipitate with alcohol, and dry to obtain iron rice bran polysaccharide, which is ready for use.

[0070] Take 20 mg of the above-mentioned rice bran polysaccharide iron and 20 mg of rhodamine B and dissolve them in 10 ml of water, then add 2 ml of pyridine, stir and react in the dark at room temperature, and form a mixed solution after reacting for 24 hours; place the mixed solution in a dialysis bag with a molecular weight...

Embodiment 2

[0072] Add 10% sodium hydroxide solution dropwise to the rice bran polysaccharide aqueous solution until the pH is 9, and add 10% ferric chloride solution dropwise to the above rice bran polysaccharide under the condition of pH 8-9 in a water bath at 60°C React in an aqueous solution to form a reaction solution until a reddish-brown precipitate appears in the reaction solution. Centrifuge the reaction solution, add absolute ethanol 4 times the volume of the supernatant to the supernatant, let it stand at 4°C for 12 hours, centrifuge the precipitate, wash the precipitate with alcohol, and dry it to obtain iron rice bran polysaccharide, which is ready for use.

[0073] Take 200 mg of the above-mentioned rice bran polysaccharide iron and 20 mg of rhodamine B and dissolve them in 10 ml of water, then add 2.5 ml of pyridine, stir and react in the dark at room temperature, and form a mixed solution after 22 hours of reaction; place the mixed solution in a dialysis bag with a molecula...

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Abstract

The invention discloses a preparation method and applications of fluorescently-labeled rice bran polysaccharide iron complex, and belongs to the technical field of tracing technology. The preparationmethod comprises following steps: rice bran polysaccharide iron complex and rhodamine B are dissolved in water, an aprotic solvent is added so as to obtain a mixed solution; the mixed solution is subjected to dialysis so as to obtain a reservation liquid; absolute ethanol is added into the reservation liquid so as to obtain a precipitate; the precipitate is washed and dried so as to obtain the fluorescently-labeled rice bran polysaccharide iron complex. According to the preparation method, reaction of the reductive end terminals in rice bran polysaccharide iron complex molecules with the active amino groups in rhodamine B can be realized, the rice bran polysaccharide iron complex-rhodamine B compound, that is to say the fluorescently-labeled rice bran polysaccharide iron complex is prepared, the preparation method is simple, and maneuverability is high. The fluorescently-labeled rice bran polysaccharide iron complex is applied in tracing of rice bran polysaccharide iron complex, so that a rapid convenient method is provided for tracing of rice bran polysaccharide iron complex is provided, and a problem in the prior art that tracing of rice bran polysaccharide iron complex is difficult is solved.

Description

technical field [0001] The invention relates to the technical field of tracing, in particular to a preparation method and application of fluorescently labeled rice bran polysaccharide iron. Background technique [0002] In-depth research on the bioactivity of rice bran polysaccharide iron has extremely important practical significance for strengthening the deep processing and high-value utilization of agricultural resources in my country, and promoting the application and development of rice bran polysaccharide iron in food and medicine. However, since most of the rice bran polysaccharide iron molecular structure does not contain co-chromogenic groups, it cannot be traced and detected by simple ultraviolet absorption spectrum or fluorescence spectrum. (HPLC) combined with differential refractive index detector (RID), mass spectrometry detector (MSD) or evaporative light scattering detector (ELSD) to analyze and detect polysaccharide iron, but this type of detection instrumen...

Claims

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Application Information

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IPC IPC(8): C08B37/00G01N21/64C09K11/06
Inventor 刘梁吴淑恒潘显虎虞鸿玉燕宇剑倪丹妮关金涛陈新
Owner WUHAN POLYTECHNIC UNIVERSITY
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