An Efficient Isolation and Culture Method of Adipose Endothelial Progenitor Cells Without Coating

A technology of endothelial progenitor cells and cells, applied in the field of obtaining endothelial progenitor cells, can solve the problems of FN and serum, cumbersome operating procedures, etc., and achieve the effect of clear ingredients, low operation requirements, strong cell stemness and value-added ability
CN109897815BActive Publication Date: 2021-04-16BEIJING JING MENG STEM CELL TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING JING MENG STEM CELL TECH
Publication Date
2021-04-16

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Abstract

The invention discloses a method for obtaining endothelial progenitor cells from human or animal autologous adipose tissue. The endothelial progenitor cells have high purity, strong cell stemness and proliferation ability, and have typical endothelial progenitor cell morphology, cell phenotype, and ability to absorb low-density lipoprotein and form blood vessels.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the separation and purification of endothelial progenitor cells, in particular to a method for obtaining endothelial progenitor cells from fat samples. Background technique

[0002] Endothelial progenitor cells (EPCs) are primitive cells that can proliferate, migrate, adhere and differentiate into vascular endothelial cells, and play an important role in repairing vascular endothelium and promoting angiogenesis. Studies have shown that EPC plays an important role in the research of cardiovascular and cerebrovascular diseases, diabetic vascular diseases, hypertension, liver cirrhosis and tumor therapeutic drugs targeting angiogenesis.

[0003] EPCs originate from bone marrow and exist in bone marrow, umbilical cord blood, and peripheral blood. The latest research shows that EPCs are also found in umbilical cord, placenta, fat, and the adventitia of organs. Currently, the main sources...

Claims

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