Preparation method of euphausia superba peptide ferrous chelate

An Antarctic krill and chelate technology is applied in the field of preparation of Antarctic krill peptide ferrous chelate, and achieves the effects of being beneficial to absorption, improving added value and utilization rate, and having a simple operation process

Inactive Publication Date: 2019-06-18
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is often reported that peptides are extracted from marine organisms by enzymatic hydrolysis, there is no relevant literature report on the use of simulated human gastrointestinal digestion method to obtain Antarctic krill peptides to prepare peptide ferrous chelates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add a mixture of n-hexane and absolute ethanol with a volume ratio of 1:1 to Antarctic krill powder to degrease, extract at 50°C for 4 hours, repeat 1-2 times, filter with suction, air-dry the obtained filter cake naturally, and pulverize to obtain Antarctic krill defatted powder; add water to the prepared Antarctic krill defatted powder to prepare a digestion reaction solution with a substrate protein concentration of 2% (w / v), shake and incubate at 37°C for 30 min at 140 rpm, and use 2 M HCl adjusted the pH to 3.5, added simulated gastric digestion solution (the mass ratio of pepsin to substrate protein was 1:50), adjusted the pH to 2.0, and digested in a water bath at 37°C for 60 min; the prepared simulated gastric Digest with 1 M NaHCO 3 Adjust the pH to 6.0, add simulated intestinal digestion solution (the mass ratio of trypsin to substrate protein is 1:200, and the mass ratio of trypsin to bile salt is 1:2), adjust the pH to 7.5, and store in a water bath at 37°C ...

Embodiment 2

[0028] Add a mixture of n-hexane and absolute ethanol with a volume ratio of 1:2 to the Antarctic krill powder to degrease, extract at 50°C for 6 h, repeat 1-2 times, filter with suction, and air-dry the obtained filter cake naturally and pulverize , to obtain Antarctic krill defatted powder; add water to the prepared Antarctic krill defatted powder to prepare a digestion reaction solution with a substrate protein concentration of 4% (w / v), and incubate at 37°C and 140 rpm for 30 min with shaking , adjusted the pH to 3.5 with 2 M HCl, added simulated gastric digestion solution (the mass ratio of pepsin to substrate protein was 1:100), adjusted the pH to 2.0, and digested in a water bath at 37°C for 90 min; the obtained Simulated gastric digest with 1 M NaHCO 3Adjust the pH to 6.0, add simulated intestinal digestion solution (the mass ratio of trypsin to substrate protein is 1:500, and the mass ratio of trypsin to bile salt is 1:5), adjust the pH to 7.5, and store in a water ba...

Embodiment 3

[0030] Add a mixture of n-hexane and absolute ethanol with a volume ratio of 1:5 to Antarctic krill powder to degrease, extract at 50°C for 4 hours, repeat 1-2 times, filter with suction, air-dry the obtained filter cake naturally, and pulverize to obtain Antarctic krill defatted powder; add water to the prepared Antarctic krill defatted powder to prepare a digestion reaction solution with a substrate protein concentration of 5% (w / v), shake and incubate at 37°C and 140 rpm for 30 min, and use 2 M HCl adjusted the pH to 3.5, added simulated gastric digestion solution (the mass ratio of pepsin to substrate protein was 1:200), adjusted the pH to 2.0, and digested in a water bath at 37°C for 120 min; the prepared simulated gastric Digest with 1 M NaHCO 3 Adjust the pH to 6.0, add simulated intestinal digestion solution (the mass ratio of trypsin to substrate protein is 1:800, and the mass ratio of trypsin to bile salt is 1:8), adjust the pH to 7.5, and store in a water bath at 37...

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Abstract

The invention discloses a preparation method of euphausia superba peptide ferrous chelate. The preparation method comprises following steps: A, preparation of euphausia superba degreased powder: an n-hexane and absolute ethyl alcohol mixed solution is added to euphausia superba powder for degreasing, an obtained filter cake is air-dried and smashed, and the euphausia superba degreased powder is obtained; B, preparation of simulated stomach digest: water is added to the prepared euphausia superba degreased powder, a digestion reaction liquid is prepared, simulated stomach digestive juice is added to the digestion reaction liquid, a euphausia superba stomach digestion reaction liquid is prepared, and the simulated stomach digest is prepared from the euphausia superba stomach digestion reaction liquid; C, preparation of euphausia superba digest: pH of the prepared simulated stomach digest is regulated by NaHCO3, simulated intestinal digestive juice is added, and enzyme deactivation and centrifugation are performed; D, preparation of the euphausia superba peptide ferrous chelate. With the adoption of the preparation method, added value and utilization rate of the euphausia superba areincreased; the operating process is simple, binding capacity of the prepared euphausia superba peptide ferrous chelate is up to 70%-90%, production efficiency is improved, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of Antarctic krill deep processing, in particular to a preparation method of Antarctic krill peptide ferrous chelate. Background technique [0002] Antarctic krill ( Euphausia superba ) is one of the largest single biological resources on the earth, with abundant reserves, and the maximum annual catch can reach 100 million tons. Because it is rich in high-quality protein, it is considered to be the largest and last potential animal protein resource pool on the earth Therefore, Antarctic krill is listed as an important economic strategic resource for development by Japan, Norway, the United Kingdom, the United States, Russia and other countries in the world. my country also attaches great importance to Antarctic krill. In 2009, the Ministry of Agriculture listed Antarctic krill as "national economic "Strategic resources", and issued a probing order, more than 1,000 tons of catches enter the Chinese market ever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/107
Inventor 孙娜沈佳颖陆雪琪林松毅王彤彤李冬梅
Owner DALIAN POLYTECHNIC UNIVERSITY
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