Sorghum 14-3-3 protein GF14c gene, recombined vector thereof and expression method of gene

A recombinant vector and gene technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve the problems that have not yet been reported on 14-3-3 protein gene research.
CN109912705APending Publication Date: 2019-06-21GUIZHOU UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
GUIZHOU UNIV
Publication Date
2019-06-21

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Abstract

The invention discloses a sorghum 14-3-3 protein GF14c gene. The nucleotide sequence of the gene is as shown in SEQ ID NO.1. The invention further discloses protein of a sorghum 14-3-3 protein GF14c gene code, and the amino acid sequence of the protein is as shown in SEQ ID NO.2. The invention further discloses a recombined vector and an expression method comprising the sorghum 14-3-3 protein GF14c gene. By the aid of a protein sequence of a corn GF14c gene, a sorghum homologous gene GF14c is acquired from a sorghum gene database, the overall length of the gene is 771bp, a prokaryotic expression vector is built according to the gene and purified by a protein purification system, and a foundation is laid for further study of the a protein crystal structure and biological characteristics bya purification result, so that bases are provided for improvement of sorghum stress resistance by a gene editing method.
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Description

technical field

[0001] The invention relates to a sorghum 14-3-3 protein GF14c gene, a recombinant vector and an expression method thereof, belonging to the field of biotechnology. Background technique

[0002] Sorghum (Sorghum bicolor (L.) Moench), also known as water millet and chestnut, is an important cereal crop with strong resistance to adversity. It is an important raw material, and it is also a kind of animal husbandry crop that has been widely used in recent years. Sorghum has a long history of cultivation in my country, and it was not cultivated on a large scale before the founding of the People's Republic of China. It was not until the 1970s, with the development of economy and society, that my country gradually paid attention to and cultivated sorghum on a large scale. Sorghum is a short-day C4 plant. Compared with other energy crops, sorghum has more obvious photosynthetic efficiency and higher yield advantages, so it is known as a "high-energy crop". In recen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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