Method for rapidly grafting green pomelo
A green pomelo, fast technology, applied in the directions of grafting, horticultural methods, botanical equipment and methods, etc., can solve the problems of slow reproduction speed, difficult to maintain good characters, high cost, shorten the seedling period, ensure the characteristics of good varieties, restore quick effect
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[0030] The grafting time of the present embodiment green pomelo is selected in March, and the grafting site is selected in a certain farm in Guilin, Guangxi, and the specific procedures are as follows:
[0031] Rootstock cultivation:
[0032] Select the young and knotted stems of green pomelo with good growth and no disease as explants, put in the disinfectant solution with the concentration of 5-10% alcohol and 5-10g / L of chitosan oligosaccharide for 20 minutes, and then inoculate Cultivate the callus on the cluster bud induction medium, then transfer the callus to the subculture medium for cultivation to obtain subculture adventitious buds, and finally transfer the subculture adventitious buds to the rooting medium for cultivation, and grow 3 After -5 roots, the rootstock tissue culture seedlings are obtained, and then the tissue culture seedlings are planted in a seedling cup equipped with a seedling growth substrate and cultivated to 10-15 cm, and then the rootstock is obt...
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