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DR5-targeting single-chain antibody, chimeric antigen receptor T cell, preparation method and application of of chimeric antigen receptor T cell

A technology of chimeric antigen receptor and single chain antibody, which is applied in the field of medical biology to achieve the effect of promoting amplification, strong affinity activity, high efficiency and specific killing

Inactive Publication Date: 2019-07-02
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no preparation and research of chimeric antigen receptor T cells targeting DR5

Method used

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  • DR5-targeting single-chain antibody, chimeric antigen receptor T cell, preparation method and application of of chimeric antigen receptor T cell
  • DR5-targeting single-chain antibody, chimeric antigen receptor T cell, preparation method and application of of chimeric antigen receptor T cell

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Experimental program
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Embodiment 1

[0075] A method for preparing chimeric antigen receptor T cells targeting DR5, comprising the following steps:

[0076] (1) Prepare the gene sequence of chimeric antigen receptor CAR-DR5 targeting DR5

[0077]The genes encoding the signal peptide, the single-chain antibody targeting DR5, the hinge region of CD8α, the transmembrane region of CD8, the signal region of CD27, and the signal region of CD3ζ were respectively prepared. The genes encoding the signal peptide are shown in SEQ ID NO: 15. The coding gene of the single-chain antibody targeting DR5 is shown in SEQ ID NO: 2, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO 9, the coding gene of the CD27 signal region is shown in SEQ ID NO: 11, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 13.

[0078] The genes encoding the above signal peptide, single-chain antibody targeting DR5, CD8α hinge region, CD8 transm...

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PUM

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Abstract

The present invention provides a DR5-targeting single-chain antibody, comprising an amino acid sequence shown in SEQ ID NO: 1. The invention also provides a chimeric antigen receptor T cell comprisingthe DR5-targeting single-chain antibody, and the DR5-targeting chimeric antigen receptor can specifically target DR5 to promote amplification of T cell in the body of a patient, thus efficiently andspecifically kill tumor cells and preferably maintain viability and killing effect of cells without damaging normal cells. The invention further provides a preparation method and application of the DR5-targeting chimeric antigen receptor T cell.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a single-chain antibody targeting DR5, a chimeric antigen receptor T cell, and a preparation method and application thereof. Background technique [0002] Cancer has become the culprit that threatens human life, and the incidence rate is on the rise. From the perspective of inducing tumor cell apoptosis or overcoming the resistance of tumor cells to apoptosis, people have discovered tumor necrosis factor-related apoptosis-inducing ligand (TRAIL ), a member of the tumor necrosis factor superfamily capable of inducing apoptosis. Death receptor 5 (death receptor5; DR5) is a member of the tumor necrosis factor receptor superfamily (TN FRSF10B), and its cytoplasmic region contains a death domain, mainly distributed in tumor cells. TRAIL binds to DR5 on the cell surface and induces apoptosis. DR5 is widely expressed in various solid tumor cells such as liver cancer, while it is very we...

Claims

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Application Information

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IPC IPC(8): C07K16/28C12N15/13C07K19/00C12N15/62C12N15/867C12N5/10A61P35/00
CPCA61K35/17C07K14/7051C07K16/2878C07K2317/24C07K2317/622C07K2319/02C07K2319/03C07K2319/33C07K2319/74C12N5/0636C12N15/86C12N2510/00C12N2740/15043C12N2800/107
Inventor 张宏玲龙丽梅钟春颖
Owner SHENZHEN BINDEBIOTECH CO LTD
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