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Preparation of dual-recognition chlorpyrifos sensor, method and detection device for detecting chlorpyrifos residues in vegetables

A chlorpyrifos and sensor technology, which is applied in the field of electrochemistry, can solve the problems of sensitivity influence, complicated instrument use, complicated sample extraction and purification steps, etc., and achieves good selectivity.

Active Publication Date: 2021-07-13
YANGZHOU POLYTECHNIC INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography can improve the sensitivity and selectivity of analysis, but the use of instruments is more complicated and the cost is higher, the steps of sample extraction and purification are relatively cumbersome, and the sensitivity is affected by steps such as sample purification and concentration, and these methods have high requirements for instruments and equipment

Method used

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  • Preparation of dual-recognition chlorpyrifos sensor, method and detection device for detecting chlorpyrifos residues in vegetables
  • Preparation of dual-recognition chlorpyrifos sensor, method and detection device for detecting chlorpyrifos residues in vegetables
  • Preparation of dual-recognition chlorpyrifos sensor, method and detection device for detecting chlorpyrifos residues in vegetables

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preparation example Construction

[0043] Preparation of Perovskite Precursor

[0044] 38mL volume percentage concentration 33% methylamine (CH 3 NH 2 ), 40mL volume percent concentration 57% hydroiodic acid (HI) and 100mL volume percent concentration 95% ethanol solution were mixed at room temperature, stirred for 1h in a nitrogen atmosphere, evaporated to obtain crystals, washed 3 times with dimethyl ether, and then Dry in a vacuum oven at 60°C for 24 hours to obtain white CH 3 NH 3 I crystal, then the resulting CH 3 NH 3 I Crystal and PbCl 2 Mix and stir in DMF for half an hour according to the molar ratio of 4:1 to obtain a brown perovskite precursor solution.

[0045] ITO / CH 3 NH 3 PB 3 Preparation of / CS / AChE Dual Recognition Sensor

[0046] Smoked tin oxide (ITO) conductive glass is cut into flakes and put into -1 In the isopropanol solution of KOH, boil for 30 minutes, clean it with ultrapure water for 10 minutes, and dry it in an oven at 100°C for later use.

[0047] ITO / CH 3 NH 3 PB 3 P...

Embodiment 1

[0068] Convert MIP / ITO / CH 3 NH 3 PB 3 / CS / AChE electrode in different concentrations of chlorpyrifos solution (0, 0.5, 1.0, 10.0nmol L -1 ) after immersing for 10 minutes, take it out, and then insert -1 0.1mol L of acetylthiocholine chloride -1 In the pH 7.0 PBS electrolytic cell, a halogen light source is set on the left side of the electrolytic cell; under the condition of a bias potential of 0V, the halogen light source irradiates the acetylthiocholine chloride electrolyte from left to right in the electrolytic cell, and measures Obtain the photocurrent inhibition signal value of the chlorpyrifos solution of different known concentrations; Take the concentration of the chlorpyrifos solution as the abscissa, and take the inhibition photocurrent signal value of the chlorpyrifos solution as the ordinate, such as figure 1 shown. from figure 1 In , it can be seen that different concentrations of chlorpyrifos solutions correspond to different inhibitory photocurrent signal...

Embodiment 2

[0070] Will ITO / CH 3 NH 3 PB 3 / CS / AChE electrode in different concentrations of chlorpyrifos solution (0, 0.02, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 and 2.0nmol L -1 ) after immersing for 10 minutes, take it out, and then insert -1 0.1mol L of acetylthiocholine chloride -1 In the pH 7.0 PBS electrolytic cell, a halogen light source is set on the left side of the electrolytic cell; under the condition of a bias potential of 0V, the halogen light source irradiates the acetylthiocholine chloride electrolyte from left to right in the electrolytic cell, and measures Obtain the photocurrent inhibition signal value of the chlorpyrifos solution of different known concentrations; Take the concentration of the chlorpyrifos solution as the abscissa, and take the inhibition photocurrent signal value of the chlorpyrifos solution as the ordinate, obtain the linear relation figure of the chlorpyrifos concentration and the electric current, as figure 2 As shown, the linear equation is I=...

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Abstract

The invention discloses a preparation method of an enzyme-molecular imprinted double-recognition chlorpyrifos sensor based on a nano-perovskite material, that is, MIP / ITO / CH 3 NH 3 PB 3 Preparation of / CS / AChE dual recognition sensor. The invention also discloses a method for detecting chlorpyrifos residues in vegetables, specifically: a. making a linear relationship diagram between chlorpyrifos concentration and current; b. testing the concentration of chlorpyrifos in a sample: measuring and obtaining the inhibitory photocurrent signal value of the chlorpyrifos solution to be tested; Using the prepared linear relationship diagram between the concentration of chlorpyrifos and the inhibition photocurrent to measure the inhibition photocurrent signal value of the chlorpyrifos solution to be tested, and obtain the concentration of chlorpyrifos in the chlorpyrifos solution to be tested. The invention also discloses a device for detecting chlorpyrifos residue in vegetables, which comprises an electrochemical workstation, a light source, an electrolytic cell, a working electrode, a reference electrode and an auxiliary electrode. The invention can detect chlorpyrifos residue in vegetables, has low detection cost, good selectivity, simple sample pretreatment, convenient operation and small sampling volume.

Description

technical field [0001] The invention relates to an electrochemical method, in particular to a method for detecting chlorpyrifos residues in vegetables, and an enzyme-molecular imprinting dual-recognition chlorpyrifos sensor based on nanometer perovskite materials. Background technique [0002] Chlorpyrifos (CPF), an organophosphate insecticide commonly used in agriculture, can destroy cholinesterase and cause choline dysfunction or even death, thereby endangering the safety of humans and animals. Limiting the use of organophosphorus pesticides in agriculture and banning the use of toxic organophosphorus pesticides are of vital importance and have received widespread attention worldwide. Recently, some toxic organophosphorus pesticides, such as parathion and methyl parathion, have been banned or severely restricted by EPA and food and agriculture organizations in the United States. In 2000, the Japanese Ministry of Health, Labor and Welfare stipulated that the concentration ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 金党琴吴耀月王珍
Owner YANGZHOU POLYTECHNIC INST