Construction method of I-type diabetes large animal model

A technology for large animals and construction methods, which is applied in the field of biomedicine and can solve problems such as primate ethics, severe viral infectious diseases, rodent morphology, and large body weight differences.

Inactive Publication Date: 2019-07-05
河南象南网络科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing problem is that rodents differ greatly from humans in terms of shape and weight, and primates bring about ethical and severe viral infectious diseases and other issues.

Method used

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  • Construction method of I-type diabetes large animal model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Animal screening

[0020] Screen 15 castrated male pigs with a fasting blood glucose level of ≦5.6mmol / L for 12 hours and a blood glucose value of ≦11.1mmol / L 1 hour after eating. Healthy castrated male pigs with a weight of 80 catties were used as simulated model animals.

[0021] (2) Injection of STZ:

[0022] After fasting for 10 hours, the screened model animals were injected with streptozotocin (STZ) at a dose of 110 mg / kg into the model animals through the intraperitoneal vein.

[0023] (3) Model screening

[0024] Pigs injected with streptozotocin (STZ) were fed with normal feed formula for 8 days, fasted for 12 hours, and blood was taken for testing. When the fasting blood sugar value was ≥12mmol / L, the animal model of diabetes could be obtained.

[0025] (4) Insulin maintenance

[0026] After the successful establishment of the type I diabetes animal model, 0.2 U / kg of body weight insulin was given every day for maintenance, and the injection was divided...

Embodiment 2

[0028] (1) Animal screening

[0029] Screen 15 castrated male pigs with a fasting 13-hour fasting blood glucose value of ≦5.6mmol / L and a 1-hour post-eating blood glucose value of ≦11.1mmol / L. Healthy castrated male pigs with a weight of 100 catties were used as simulated model animals.

[0030] (2) Injection of STZ:

[0031] After fasting for 11 hours, the screened model animals were injected with streptozotocin (STZ) at a dose of 120 mg / kg into the model animals through the intraperitoneal vein.

[0032] (3) Model screening

[0033] Pigs injected with streptozotocin (STZ) were fed with normal feed formula for 9 days, fasted for 13 hours, and blood samples were taken for testing. When the fasting blood glucose value was ≥12mmol / L, the animal model of diabetes could be obtained.

[0034] (4) Insulin maintenance

[0035] After the successful modeling of type I diabetes animal model, 0.3 U / kg body weight insulin was given every day for maintenance, and the injection was divid...

Embodiment 3

[0037] (1) Animal screening

[0038] Screen 15 healthy castrated male pigs weighing 120 catties with a fasting blood glucose level of ≦5.6mmol / L for 15 hours and a blood glucose value of ≦11.1mmol / L 1 hour after eating as the model animals.

[0039] (2) Injection of STZ:

[0040] After fasting for 12 hours, the screened model animals were injected with streptozotocin (STZ) at a dose of 130 mg / kg into the model animals through the intraperitoneal vein.

[0041] (3) Model screening

[0042] Pigs injected with streptozotocin (STZ) were fed with normal feed formula for 10 days, fasted for 15 hours, and blood samples were taken for testing. When the fasting blood sugar value was ≥12mmol / L, the animal model of diabetes could be obtained.

[0043] (4) Insulin maintenance

[0044] After the successful establishment of the type I diabetes animal model, 0.4 U / kg of body weight insulin was given every day for maintenance, and the injection was divided into 3 times, and the time was wi...

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Abstract

The invention discloses a construction method of an I-type diabetes large animal model, and belongs to the technical field of biomedical therapies. According to the construction method, pigs with widesources serve as modeling experimental animals of the I-type diabetes large animal model and are injected with a dosage of 110-130 mg/kg streptozotocin, pig pancreas beta cells are selectively damaged to suffer from autoimmune destruction to cause insulin deficiency, and thus the I-type diabetes large animal model is constructed. The construction method has the advantages of being convenient in modeling, low in cost, wide in source and high in similarity, animal survival rate and model establishing success rate, and provides ideal experimental animal models for pathogenesis, treatment means and drug development and use of I-type diabetes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for constructing a large animal model of type I diabetes. Background technique [0002] Diabetes is a group of metabolic diseases characterized by high blood sugar. Diabetes is divided into type I diabetes and type II diabetes. Type I diabetes, also known as insulin-dependent diabetes, is an autoimmune disease. Due to the destruction of pancreatic beta cells, Leading to absolute lack of insulin secretion, patients need life-long injections of exogenous insulin to control blood sugar. [0003] If blood sugar is not well controlled for a long time in diabetic patients, it can lead to organ tissue damage and become the "source of all diseases", accompanied by various organs, especially eye, heart, blood vessel, kidney, nerve damage or organ insufficiency or failure. The patient becomes disabled or dies. [0004] Currently, animal models of type I diabetes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 王昆朋
Owner 河南象南网络科技有限公司
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