Saccharomyce sp. ZB412 and application thereof

A ZB412, yeast technology, applied in the direction of fermentation, fungi, microorganisms, etc., can solve the problems of increasing production energy consumption and production cost, unable to meet the demand for vinegar flavor, and the generation of unfavorable aroma substances, so as to improve the utilization rate of raw materials , good operability and economic benefits, good taste and sweetness

Active Publication Date: 2019-07-09
FOSHAN HAITIAN GAOMING FLAVORING & FOOD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese Invention Patent No. CN201510849370.7 discloses a strain of yeast with high ethyl acetate production under low temperature conditions and its application. The ethyl acetate production of the strain reaches the maximum under 22°C and static fermentation conditions, and the fermentation metabolites are only Contains ethyl acetate and a small amount of ethanol. The yeast strain above 28°C has low activity of producing ethyl acetate. When it is used in the production and fermentation of vinegar, it is necessary to reduce the fermentation temperature of the traditional production process, which increases the production energy consumption and production cost.
[0005] Due to the limitations of the technical conditions of the liquid deep-layer fermentation vinegar, it is not conducive to the generation of aroma substances such as organic acids, esters, and free amino acids, resulting in the problems of insufficient flavor and poor taste in the fermented vinegar, which cannot satisfy people's desire for vinegar. flavor needs
Therefore, in the prior art, there is no safe bacteria that can be directly applied in the process of producing vinegar by liquid submerged fermentation, and then increase the content of 4-ethylguaiacol and ethyl acetate in the fermentation product of the vinegar fermentation stage. Improving the Flavor Quality of Vinegar Safely and Reliably

Method used

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  • Saccharomyce sp. ZB412 and application thereof
  • Saccharomyce sp. ZB412 and application thereof
  • Saccharomyce sp. ZB412 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 strain mutagenesis

[0042] Saccharomyces ZB118 is used as the starting strain, and the acquisition of yeast ZB118 refers to the method described in the Chinese patent "A Yeast and Its Application" with publication number CN108315271A. The biological deposit information of yeast ZB118 is as follows: it was deposited in the Guangdong Provincial Microbial Culture Collection Center on November 17, 2017, named as yeast (saccharomyce sp.) ZB118, the deposit number is GDMCC NO:60280, and the deposit address is Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou. The mature slant of the strain ZB118 was made into a bacterial suspension, and the yeast suspension was subjected to ARTP mutagenesis. Dilute the mutagenized yeast suspension to 10 with sterile saline -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Concentration, make bacterial cell dilution, count the bacterial cell dilution of different concentrations under the microscope, choose 10 -3 Concentra...

Embodiment 2

[0043] The screening of embodiment 2 mutagenic strains

[0044] (1) Primary screening of mutagenized strains

[0045] Pipette 0.1 mL of the bacterial cell dilution obtained in Example 1, and evenly spread it on the YPD solid medium supplemented with 0.4% tributyrin, and incubate at 28-30°C in the dark for 48 hours, and observe the growth of colonies during the cultivation process The situation and the speed of the formation of the transparent circle, and record the diameter of the colony growth and the diameter of the transparent circle. The ratio of the diameter of the transparent circle to the diameter of the colony is used to characterize the ester-producing ability of the yeast strain. Therefore, screening is carried out according to the ratio of the diameter of the transparent circle to the diameter of the colony. Compared with the starting strain, the colony has good colony growth and better ester production performance. After screening, six strains with better ester-p...

Embodiment 3

[0058] Example 3 Saccharomyces ZB412 strain genetic stability detection

[0059] The yeast strain ZB412 screened out in Example 2 was inoculated on the YPD slant medium for 10 generations, and the growth of each generation strain was observed, and the 1st generation, the 5th generation and the 10th generation slant strain were inoculated according to Example 2. According to the method in the middle method, the strains were fermented and cultivated. After the fermentation, the contents of 4-ethylguaiacol and ethyl acetate in the fermentation liquid were determined by high-performance liquid chromatography, and the genetic stability was judged. If the content error of 4-ethylguaiacol and ethyl acetate in the obtained fermentation broth is within 10%, it indicates that the genetic stability of the strain is good, and the specific data are shown in Table 3.

[0060] Table 3 Yeast strain subculture stability test results

[0061]

[0062] As shown in the results in Table 3, fro...

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Abstract

The invention discloses a saccharomyce sp. ZB412. The saccharomyce sp. ZB412 was preserved in Guangdong Culture Collection Center on December 12, 2018 with a preservation number of GDMCC NO: 60523. The invention also discloses application of the saccharomyce sp. ZB412 to food fermentation. The saccharomyce sp. ZB412 obtained through strain mutagenesis can significantly improve the content of 4-ethyl guaiacol and ethyl acetate in liquid-state fermented vinegar. The saccharomyce sp. ZB412 is high in acid resistance and can significantly increase the utilization rate of raw materials of the vinegar and the content of amino nitrogen and total ester when inoculated into sweet mash for alcoholic fermentation; the prepared vinegar is high in flavor purity, moderate in acidity, outstanding in ester flavor and good in taste and sweetness. The saccharomyce sp. ZB412 can be applied as a fermentation functional bacterium to traditional fermentation industries such as vinegar, soybean sauce and fermented wine as well as the food industry, thereby ensuring that products are outstanding in ester flavor, significantly improving the quality of the products and achieving high operability and economic benefits.

Description

technical field [0001] The invention relates to the technical field of food fermentation, in particular to a saccharomyce sp. suitable for the production of vinegar and rice vinegar, and also relates to the application of the saccharomyce sp. in the production of vinegar. Background technique [0002] At present, the production of vinegar on the market is mainly divided into traditional solid-state fermentation technology and liquid deep-layer fermentation technology. The vinegar produced by traditional solid-state fermentation has a unique flavor and soft sour taste, but the traditional solid-state technology has labor intensity, low production efficiency, and production cycle However, the liquid submerged fermentation process can obtain vinegar products after rapid fermentation by yeast and acetic acid bacteria. It has the advantages of stable product, less precipitation, short fermentation cycle, high degree of mechanization and low cost, and has become a vinegar productio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12J1/04C12G3/022A23L27/50C12P7/62C12P7/22C12R1/85
CPCA23L27/50C12G3/02C12J1/04C12P7/22C12P7/62C12N1/185C12R2001/85
Inventor 周其洋崔谷梨周斌童星
Owner FOSHAN HAITIAN GAOMING FLAVORING & FOOD
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