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A method for evaluating the exposure risk of tobacco-specific nitrosamines to animals

A technology based on exposure risk and animal body, applied in the field of carcinogen exposure risk assessment, can solve problems such as inability to quantify the potential exposure risk of TSNAs

Active Publication Date: 2021-12-17
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for evaluating the exposure risk of tobacco-specific nitrosamines to animal bodies, so as to solve the problem that the existing methods cannot quantitatively evaluate the potential exposure risk of TSNAs

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  • A method for evaluating the exposure risk of tobacco-specific nitrosamines to animals
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  • A method for evaluating the exposure risk of tobacco-specific nitrosamines to animals

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Embodiment Construction

[0032] The present invention mainly uses a certain dose of representative TSNAs (NNK, NNAL, NNN, NAB) solution to expose animal models, and uses high performance liquid chromatography-tandem mass spectrometry to measure the direct metabolites of TSNAs in blood and the indirect metabolites in liver tissue , on the basis of obtaining the relative amount of TSNAs and their metabolites entering the systemic circulation, according to the ratio of the total amount of α-hydroxylation metabolic pathway products in the blood to the relative amount of TSNAs entering the systemic circulation (α-hydroxylation coefficient) and the main metabolic organ The production of representative DNA adducts within the body was used to assess the degree of α-hydroxylation in different individuals after exposure to TSNAs.

[0033] On the one hand, this method uses the ratio of direct metabolites in the blood to reflect the average degree of α-hydroxylation metabolism of TSNAs in the body; The degree of ...

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Abstract

The invention relates to a method for evaluating the exposure risk of tobacco-specific nitrosamines to animal bodies. The method comprises the following steps: 1) detecting the content of TSNAs and metabolites thereof; calculating the AUC value, and calculating the α-hydroxylation coefficient according to the AUC value; detecting O 6 ‑mG and O 2 ‑POB‑dT content; based on detected O 6 ‑mG and O 2 The sum of the content values ​​of ‑POB‑dT to obtain the yield of representative DNA adducts; 2) According to the α‑hydroxylation coefficient and the yield of representative DNA adducts, evaluate the degree of α‑hydroxylation metabolism and predict the effect of TSNAs on animals exposure risk. This method evaluates the degree of α-hydroxylation reaction in different individuals after exposure to TSNAs based on the α-hydroxylation coefficient and the yield of representative DNA adducts, and then provides quantitative indicators for evaluating the potential exposure risk of TSNAs.

Description

technical field [0001] The invention belongs to the field of exposure risk assessment of carcinogens, and in particular relates to a method for assessing the exposure risk of tobacco-specific nitrosamines (TSNAs) to animal bodies. Background technique [0002] TSNAs are a unique group of N-nitrosamine harmful components in tobacco, which are formed by nitrosation of tobacco alkaloids in the process of tobacco preparation and fermentation, and have always been a hot spot in the research of smoking and health issues. There are eight known TSNAs, NNK, NNN, NAB, NAT, NNAL, iso-NNAL, NNA, and iso-NNAC. NAB and NAT are directly formed by the nitrosation of anatabine and anatabine, and NNN is mainly formed by the nitrosation of nornicotine, while the nitrosation of nicotine mainly forms NNK and is further converted into a small amount of NNAL, and a very small part Nicotine nitrosation can also form NNA and be further converted into iso-NNAL or iso-NNAC. At present, it is believe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 张建勋毛健刘俊辉孟庆华冯明飞赵贝贝郎海磊张启东卢斌斌王磊刘珊范武史清照
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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