Fingerprint detection method of licorice juice-roasted Ligustrum lucidum

The technology of a fingerprint and detection method is applied in the fingerprint detection method and the field of fingerprints of licorice juice-roasted Ligustrum lucidum, which can solve the problems of no clear regulations on process conditions and quality standards, lack of process parameters, etc., and achieve a simple and convenient method. Optimized processing technology, good reproducible effect

Active Publication Date: 2021-06-18
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the current relevant drug standards, the processing specifications of Ligustrum lucidum decoction pieces include Ligustrum lucidum and wine Ligustrum lucidum, but the specific process conditions and quality standards are not clearly stipulated, and there is a lack of objective process parameters.

Method used

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  • Fingerprint detection method of licorice juice-roasted Ligustrum lucidum
  • Fingerprint detection method of licorice juice-roasted Ligustrum lucidum
  • Fingerprint detection method of licorice juice-roasted Ligustrum lucidum

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Instruments used in the following examples

[0044] Shimadzu LC-20AB high performance liquid chromatography system from Shimadzu, Japan, including online degasser, automatic sampler Prominence SIL-20A, diode array detector SPD-M20A and column thermostat CTO-20A; ME204E electronic analytical balance (Mei Teller-Toledo International Co., Ltd., accurate to 1 / 100,000); BSM220.4 analytical balance (Shanghai Zhuojing Company, accurate to 1 / 10,000); GVS ultrasonic cleaner (Shenzhen Gowell Technology Co., Ltd. Company); TGL-16C high-speed desktop centrifuge (Shanghai Anting Scientific Instrument Factory).

[0045] The reagents used in the following examples are as follows: the standard product for the analysis of privetin (batch number 5937, with a purity of 99.5%) was purchased from Shanghai Shidande Standard Technology Service Co., Ltd. Liquiritin (batch number 111610-200604) and ammonium glycyrrhizinate (batch number 110731-201619) reference substances were purchased from C...

Embodiment 2

[0063] The methodological research of embodiment 2 fingerprint spectrum detection:

[0064] 1. Methodological investigation

[0065] 1.1 Inspection of precision

[0066] Get batch number and be 181202 the licorice juice juice broiled Ligustrum lucidum sample, according to the preparation method of the test sample of the above-mentioned embodiment 1, prepare the test sample solution, continuous sampling 6 times, each injection volume 10 μ L, according to the above-mentioned embodiment 1 Chromatographic conditions detection, determination of its HPLC chromatogram, investigation of 6 common fingerprint peaks in the chromatogram, the results show that the retention time RSD of the common fingerprint peaks is less than 1.9%, and the common peak area RSD is less than 1.7%, indicating that the instrument is precise The degree is better.

[0067] 1.2 Stability investigation

[0068] Get batch number and be 181202 the licorice juice Zhizi decoction piece sample that broils, prepare ...

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Abstract

The invention discloses a detection method for fingerprints of Ligustrum lucidum licorice juice, the method comprises the following steps: Step 1, preparation of a test solution of Ligustrum lucidum licorice juice; step 2, preparation of a mixed reference solution; step 3, Precisely draw the quantitative test sample and reference solution into the liquid chromatograph, and record the chromatogram; step 4, export the fingerprint instrument of Ligustrum lucidum licorice juice, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system, select different The chromatographic peaks that exist in the chromatograms of batches of licorice juice-burned Ligustrum lucidum are used as common peaks, and the control fingerprints of licorice juice-burned Ligustrum lucidum are generated by the average value calculation method, and the relative retention time and relative peak area of ​​each common peak are calculated. . The fingerprints of the Ligustrum lucidum roasted with licorice juice studied by the present invention can comprehensively and objectively evaluate the quality of the Ligustrum lucidum roasted with licorice juice. The detection method provided by the invention has the advantages of simple method, high accuracy, good repeatability, high precision and the like.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for detecting fingerprints of Ligustrum lucidum dried with licorice juice and the fingerprints thereof. Background technique [0002] Ligustrum lucidum Ait. is the dry ripe fruit of Ligustrum lucidum Ait. It has the effects of nourishing liver and kidney, improving eyesight and black hair. For liver and kidney yin deficiency, dizziness and tinnitus, soreness of the waist and knees, premature graying of the beard and hair, dark eyes, internal heat and quenching thirst, bone steaming and hot flashes. Licorice is the dry root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the leguminous plant Glycyrrhiza uralensis Fisch. The nature and flavor are sweet and flat, and have the effects of invigorating the spleen and replenishing qi, relieving spasms and relieving pain, and harmonizi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8631G01N30/8686
Inventor 蔡宝昌李国维金俊杰李轩涛秦昆明丁斐郑艳萍申海宁
Owner NANJING HAICHANG CHINESE MEDICINE GRPCO LTD
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