QuEChERS one-step type pretreatment device

Abstract

The invention discloses a QuEChERS one-step type pretreatment device. The QuEChERS one-step type pretreatment device comprises an outer sleeve 1 and an inner sleeve 2 inserted into the outer sleeve; an outer tube thread 1-1 is arranged on the inner wall of an opening end of the outer sleeve 1; an inner tube thread 2-1 matched with the outer tube thread 1-1 is arranged on the outer wall of the inner sleeve, a movable piston 3 is arranged at the lower end, and a filter net 4 is arranged in the middle; an inner tube small hole 2 is formed in the tube wall between the piston 3 and the filter net 4; and the piston 3 is in sealing fit with the inner wall of the inner sleeve 2. According to the device, effective extraction of the medicine in the blood of a biological sample and purification of ablood matrix can be successfully realized, the analysis sample can be obtained in one step, and instrument analysis can be directly carried out. According to the device, the operations of the QuEChERSmethod can be completed only in the sleeve, the material is saved, and the space is saved; the device is a high-efficiency and environment-friendly pretreatment analysis device which can be widely applied to sample pretreatment of forensic medicine and forensic clinical analysis.

Description

technical field [0001] The invention relates to the field of pretreatment devices for amphetamine drug analysis, in particular to a QuEChERS one-step pretreatment device. Background technique [0002] As the most routine clinical biological sample, blood can accurately and timely reflect the concentration of drugs in the body and the status of each drug in the target organ. , non-protein nitrogenous compounds (nitrogenous substances in the blood other than protein, mainly urea, uric acid, creatine, creatinine, amino acids, ammonia, peptides, bilirubin), sugar and other organic substances, vitamins, lipids (including Steroid hormones) and other organic substances often interfere with the analysis of target substances in instrumental analysis. At present, QuEChERS is a very efficient processing method for the processing of complex matrices. [0003] The essence of QuEChERS is the derivation and further development of solid phase extraction technology and matrix solid phase di...

AI Technical Summary

Problems solved by technology

However, all the above-mentioned reagents are used to improve and optimize the reagents in the QuECh ERS method. In the process of processing, numerous transfer and pipetting processes are still unavoidable, and more consumables are required, and there will be obvious system errors that will affect the experimental results.

For the QuEChERS processing device, there is an invention that uses a centrifuge tube for extraction, purification, and separation. The patent CN 103698195 B "QuEChERS method package for efficient homogeneous extraction of pesticide and veterinary drug residues" uses fruits and vegetables, meat, aquatic products, grain, wine, and tea. , The extraction tube in the method bag in the invention of tobacco, the syringe membrane filter, and the salting bag tool used can only make the extracted liquid squeezed out from the lower layer by air pressure after extraction. The nozzle will be blocked, the separation effect of the matrix and the extract will be poor, and the subsequent transfer and pipetting will cause large errors

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