Application of stropharia rugoso-annulata polysaccharide as well as plant defense activator and application
An immune inducer and technology of Stropharia stropharia, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as functional studies that have not been reported, and achieve the purpose of promoting rice growth, improving seedling quality, and preventing good effect
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Embodiment 1
[0032] Preparation of Stropharia stropharii polysaccharides, using a compound enzyme method to extract stropharia stropharii polysaccharides, the specific steps are as follows:
[0033] 1. Wash the fruiting bodies of Stropharia stropharia, dry them, grind them into powder, and pass through an 80-mesh sieve to obtain Stropharia stropharia powder;
[0034] 2. Weigh 20g of the sifted Stropharia powder, put it into a beaker, add 580mL of distilled water and mix well, then place it in a 55°C water bath to preheat;
[0035] 3. Mix papain, cellulase, and pectinase in a mass ratio of 2:1:1 to obtain a mixed enzyme, weigh 0.12 g of the mixed enzyme, dissolve it in 5 mL of distilled water, and then add the preheated aqueous solution of Stropharia stropharia powder ;
[0036] 4. After 2 hours in a water bath at 55°C;
[0037] 5. After the reaction is over, put the triangular flask into a boiling water pot, take a boiling water bath for 10 minutes to inactivate the enzyme, and then cool...
Embodiment 2
[0055] Treatment of rice seedlings with polysaccharides from Stropharia grandis.
[0056] The hybrid rice variety used in the experiment: 'Zhongzheyou 1', provided by Zhejiang Wuwangnong Seed Industry Co., Ltd.
[0057] The specific method is as follows:
[0058] 1. Weigh 10g of 'Zhongzheyou No. 1' rice seeds, pour 200mL of ultra-pure water into the rice cultivation box, and soak the seeds in a rice cultivation box at 30°C for one day;
[0059] 2. Pour off the upper layer of water and unqualified seeds that float up, wash the seeds twice, moisten the gauze, spread on the surface of the seeds, and put them in a 30°C incubator for one day to accelerate germination;
[0060] 3. Prepare 0.35% agar solution, boil it on the induction cooker for 10 minutes until there are no obvious solid particles;
[0061] 4. When the temperature is lowered to 40°C, add the Stropharia stropharii polysaccharide prepared in Example 1 to be mixed with a rice agar medium containing 100ppm polysacchar...
Embodiment 3
[0070] 1. Test materials
[0071] Hybrid rice variety: 'Zhongzheyou No. 1', provided by Zhejiang Wuwangnong Seed Industry Co., Ltd.
[0072] A plant immune inducer: based on 1 kilogram of total weight, the consumption of each component is:
[0073] Tween-20 0.01g;
[0074] Stropharia polysaccharide 0.1~0.15g;
[0075] Potassium sorbate 0.006g;
[0076] The balance is water.
[0077] The preparation method of Stropharia grandis polysaccharide in this embodiment is the same as that in Example 1.
[0078] 2. Administration of immune inducers
[0079] Beginning in early June 2018, field experiments were conducted at the Fuyang Rice Experimental Base of the China Rice Research Institute. The field planting size is 30 cm × 18 cm, each treatment is 10 square meters, and there are 3 repetitions. After the jointing stage, spray the immune inducer on the leaves once a week, stop spraying after heading, and spray 4 times in total. At the same time set chemical pesticides and wate...
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