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Application of stropharia rugoso-annulata polysaccharide as well as plant defense activator and application

An immune inducer and technology of Stropharia stropharia, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as functional studies that have not been reported, and achieve the purpose of promoting rice growth, improving seedling quality, and preventing good effect

Active Publication Date: 2019-08-02
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing research results show that Stropharia polysaccharide can enhance the immune function of animals, and has anti-tumor, anti-oxidation, and fatigue-relieving effects, but there is no report on its function in inducing plant resistance

Method used

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  • Application of stropharia rugoso-annulata polysaccharide as well as plant defense activator and application
  • Application of stropharia rugoso-annulata polysaccharide as well as plant defense activator and application
  • Application of stropharia rugoso-annulata polysaccharide as well as plant defense activator and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Stropharia stropharii polysaccharides, using a compound enzyme method to extract stropharia stropharii polysaccharides, the specific steps are as follows:

[0033] 1. Wash the fruiting bodies of Stropharia stropharia, dry them, grind them into powder, and pass through an 80-mesh sieve to obtain Stropharia stropharia powder;

[0034] 2. Weigh 20g of the sifted Stropharia powder, put it into a beaker, add 580mL of distilled water and mix well, then place it in a 55°C water bath to preheat;

[0035] 3. Mix papain, cellulase, and pectinase in a mass ratio of 2:1:1 to obtain a mixed enzyme, weigh 0.12 g of the mixed enzyme, dissolve it in 5 mL of distilled water, and then add the preheated aqueous solution of Stropharia stropharia powder ;

[0036] 4. After 2 hours in a water bath at 55°C;

[0037] 5. After the reaction is over, put the triangular flask into a boiling water pot, take a boiling water bath for 10 minutes to inactivate the enzyme, and then cool...

Embodiment 2

[0055] Treatment of rice seedlings with polysaccharides from Stropharia grandis.

[0056] The hybrid rice variety used in the experiment: 'Zhongzheyou 1', provided by Zhejiang Wuwangnong Seed Industry Co., Ltd.

[0057] The specific method is as follows:

[0058] 1. Weigh 10g of 'Zhongzheyou No. 1' rice seeds, pour 200mL of ultra-pure water into the rice cultivation box, and soak the seeds in a rice cultivation box at 30°C for one day;

[0059] 2. Pour off the upper layer of water and unqualified seeds that float up, wash the seeds twice, moisten the gauze, spread on the surface of the seeds, and put them in a 30°C incubator for one day to accelerate germination;

[0060] 3. Prepare 0.35% agar solution, boil it on the induction cooker for 10 minutes until there are no obvious solid particles;

[0061] 4. When the temperature is lowered to 40°C, add the Stropharia stropharii polysaccharide prepared in Example 1 to be mixed with a rice agar medium containing 100ppm polysacchar...

Embodiment 3

[0070] 1. Test materials

[0071] Hybrid rice variety: 'Zhongzheyou No. 1', provided by Zhejiang Wuwangnong Seed Industry Co., Ltd.

[0072] A plant immune inducer: based on 1 kilogram of total weight, the consumption of each component is:

[0073] Tween-20 0.01g;

[0074] Stropharia polysaccharide 0.1~0.15g;

[0075] Potassium sorbate 0.006g;

[0076] The balance is water.

[0077] The preparation method of Stropharia grandis polysaccharide in this embodiment is the same as that in Example 1.

[0078] 2. Administration of immune inducers

[0079] Beginning in early June 2018, field experiments were conducted at the Fuyang Rice Experimental Base of the China Rice Research Institute. The field planting size is 30 cm × 18 cm, each treatment is 10 square meters, and there are 3 repetitions. After the jointing stage, spray the immune inducer on the leaves once a week, stop spraying after heading, and spray 4 times in total. At the same time set chemical pesticides and wate...

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Abstract

The invention discloses novel application of stropharia rugoso-annulata polysaccharide as well as a plant defense activator with the stropharia rugoso-annulata polysaccharide as an active ingredient and application. Firstly, the invention effectively proves that the stropharia rugoso-annulata polysaccharide can promote the growth of rice and improve the quality of seedlings through indicators suchas plant height, dry weight, leaf age, chlorophyll content and root vitality; secondly, the plant defense activator disclosed by the invention can increase the number of grains per ear and the setting rate, so that yield increase of the rice is realized; thirdly, the plant defense activator disclosed by the invention has good control effects on the rice sheath blight and ear rot disease and is safe for rice at treatment concentration.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a stropharia polysaccharide, a plant immune inducer and its application. Background technique [0002] Rice sheath blight is a fungal disease caused by Rhizoctonia solani infection, which occurs widely in my country. After the onset, rice leaves die, the seed setting rate decreases, the thousand-grain weight decreases, and there are more grains. Generally, the yield is reduced by 10-30%, and in severe cases, the yield is reduced by 50%. At present, it has become the disease with the largest occurrence area of ​​rice in my country. [0003] For the prevention and control of this disease, Jinggangmycin, propiconazole, diniconazole, hexaconazole, etc. are often used for prevention and control, but Jinggangmycin does not last long and is highly toxic in high-quality concentrations, which may affect soil microorganisms. cause irreversible effects. Diniconazole, propi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P21/00A01P3/00C12P19/14C12P19/04
CPCA01N43/16C12P19/04C12P19/14
Inventor 肖文斐阮松林忻雅裘劼人柴伟国陈佳滢
Owner HANGZHOU ACAD OF AGRI SCI
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