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Blueberry anthocyanin-chitosan oligosaccharide complex, preparation method thereof and application of complex

A technology of blueberry anthocyanins and chitosan oligosaccharides is applied in the directions of preparation, application, and sugar derivatives of sugar derivatives, which can solve problems such as poor stability of blueberry anthocyanins, and achieve the effects of improving stability and broad application prospects.

Active Publication Date: 2019-08-16
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The inventors have developed an improved process that extracting or creating new types of flavonoid gains called blueberryanthrone (G) which could potentially provide useful medications such as cancer treatment agents. These processes use specific chemical reactions instead of traditional methods like acidic degradation techniques involving harsh acids at high temperatures. By combining these chemically modified ingredients together they make it more effective against tumours than just one type alone. Overall this innovative technology provides valuable resources for producing healthy products while also providing technical benefits related to its ability to treat diseases caused by harmful substances found naturally occurring within seafood fishes and other sources.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving stability and preservation of bluebellum flavonoids while maintaining its beneficial effects for human consumption or use purposes through different ways like making it easier to absorb and make better nutrition choices.

Method used

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  • Blueberry anthocyanin-chitosan oligosaccharide complex, preparation method thereof and application of complex
  • Blueberry anthocyanin-chitosan oligosaccharide complex, preparation method thereof and application of complex
  • Blueberry anthocyanin-chitosan oligosaccharide complex, preparation method thereof and application of complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] First, pick out frozen blueberries that are plump, free of pests and mechanical damage, rinse them with clean water, thaw them at a low temperature at 4°C, put them in a juicer to squeeze and crush them, and prepare to weigh 5g of fruit pulp. Add 60% hydrochloric acid acidified ethanol solution with a volume fraction of 1:10, adjust the pH value to 3, assisted extraction by ultrasonic oscillation for 90 minutes (set power at 500W), extraction temperature is 40°C, collect the filtrate, and concentrate under reduced pressure at room temperature A red crude extract was obtained. The crude extract obtained above was purified through AB-8 macroporous resin. AB-8 macroporous resin was activated with absolute ethanol and placed for 24 hours to make it swell, gently stirred with a glass rod to remove the resin on the surface, washed repeatedly with distilled water until no alcohol smell, soaked in 3% hydrochloric acid for 1d, Soak in 3% NaOH solution for 1d, and then wash with...

Embodiment 2

[0067] First, pick out frozen blueberries that are plump, free of pests and mechanical damage, rinse them with clean water, thaw them at a low temperature at 4°C, put them in a juicer to squeeze and crush them, and prepare to weigh 5g of fruit pulp. Add 50% hydrochloric acid acidified ethanol solution with a volume fraction of 1:5, adjust the pH value to 2, assisted extraction with ultrasonic oscillation for 60 minutes (set power is 500W), and the extraction temperature is 35°C, collect the filtrate, and concentrate under reduced pressure at room temperature A red crude extract was obtained. The crude extract obtained above was purified by D101 macroporous resin. D101 macroporous resin was activated with absolute ethanol and placed for 24 hours to make it swell, stirred gently with a glass rod to remove the resin on the surface, washed repeatedly with distilled water until no alcohol smell, soaked in 3% hydrochloric acid for 1 day, and then used Soak in 3% NaOH solution for 1...

Embodiment 3

[0073] First, pick out frozen blueberries that are plump, free of pests and mechanical damage, rinse them with clean water, thaw them at a low temperature at 4°C, put them in a juicer to squeeze and crush them, and prepare to weigh 5g of fruit pulp. Add 70% hydrochloric acid acidified ethanol solution with a volume fraction of 1:15, adjust the pH value to 4, assisted extraction with ultrasonic oscillation for 120 minutes (set power at 500W), and extract at a temperature of 45°C, collect the filtrate, and concentrate under reduced pressure at room temperature A red crude extract was obtained. The crude extract obtained above was purified through X-5 macroporous resin. X-5 macroporous resin is activated with absolute ethanol and placed for 24 hours to make it swell, gently stirred with a glass rod to remove the resin on the surface, washed repeatedly with distilled water until there is no alcohol smell, soaked in 3% hydrochloric acid for 1d, Soak in 3% NaOH solution for 1d, and...

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Abstract

The invention relates to a blueberry anthocyanin-chitosan oligosaccharide complex, a preparation method thereof and an application of the complex, and belongs to the field of food processing. The preparation method of the blueberry anthocyanin-chitosan oligosaccharide complex includes the process steps: uniformly mixing blueberry anthocyanin solution and chitosan oligosaccharide solution to obtainmixed solution; adding glutaraldehyde into the mixed solution to perform reaction to obtain blueberry anthocyanin-chitosan oligosaccharide complex solution; drying the blueberry anthocyanin-chitosanoligosaccharide complex solution to obtain blueberry anthocyanin-chitosan oligosaccharide complex solid powder. According to the method, blueberry anthocyanin extracted from natural fruit blueberriesand animal by-product shrimp and crab shell enzymolysis product chitosan oligosaccharide are effectively compounded, the complex is free from toxicity and odor, has the function of resisting tumors and potential research values in the field of medicines and can serve as a natural anti-tumor complex to be used for functional foods.

Description

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Claims

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Application Information

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Owner SHENYANG AGRI UNIV
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