Blueberry anthocyanin-chitosan oligosaccharide complex, preparation method thereof and application of complex
A technology of blueberry anthocyanins and chitosan oligosaccharides is applied in the directions of preparation, application, and sugar derivatives of sugar derivatives, which can solve problems such as poor stability of blueberry anthocyanins, and achieve the effects of improving stability and broad application prospects.
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Embodiment 1
[0061] First, pick out frozen blueberries that are plump, free of pests and mechanical damage, rinse them with clean water, thaw them at a low temperature at 4°C, put them in a juicer to squeeze and crush them, and prepare to weigh 5g of fruit pulp. Add 60% hydrochloric acid acidified ethanol solution with a volume fraction of 1:10, adjust the pH value to 3, assisted extraction by ultrasonic oscillation for 90 minutes (set power at 500W), extraction temperature is 40°C, collect the filtrate, and concentrate under reduced pressure at room temperature A red crude extract was obtained. The crude extract obtained above was purified through AB-8 macroporous resin. AB-8 macroporous resin was activated with absolute ethanol and placed for 24 hours to make it swell, gently stirred with a glass rod to remove the resin on the surface, washed repeatedly with distilled water until no alcohol smell, soaked in 3% hydrochloric acid for 1d, Soak in 3% NaOH solution for 1d, and then wash with...
Embodiment 2
[0067] First, pick out frozen blueberries that are plump, free of pests and mechanical damage, rinse them with clean water, thaw them at a low temperature at 4°C, put them in a juicer to squeeze and crush them, and prepare to weigh 5g of fruit pulp. Add 50% hydrochloric acid acidified ethanol solution with a volume fraction of 1:5, adjust the pH value to 2, assisted extraction with ultrasonic oscillation for 60 minutes (set power is 500W), and the extraction temperature is 35°C, collect the filtrate, and concentrate under reduced pressure at room temperature A red crude extract was obtained. The crude extract obtained above was purified by D101 macroporous resin. D101 macroporous resin was activated with absolute ethanol and placed for 24 hours to make it swell, stirred gently with a glass rod to remove the resin on the surface, washed repeatedly with distilled water until no alcohol smell, soaked in 3% hydrochloric acid for 1 day, and then used Soak in 3% NaOH solution for 1...
Embodiment 3
[0073] First, pick out frozen blueberries that are plump, free of pests and mechanical damage, rinse them with clean water, thaw them at a low temperature at 4°C, put them in a juicer to squeeze and crush them, and prepare to weigh 5g of fruit pulp. Add 70% hydrochloric acid acidified ethanol solution with a volume fraction of 1:15, adjust the pH value to 4, assisted extraction with ultrasonic oscillation for 120 minutes (set power at 500W), and extract at a temperature of 45°C, collect the filtrate, and concentrate under reduced pressure at room temperature A red crude extract was obtained. The crude extract obtained above was purified through X-5 macroporous resin. X-5 macroporous resin is activated with absolute ethanol and placed for 24 hours to make it swell, gently stirred with a glass rod to remove the resin on the surface, washed repeatedly with distilled water until there is no alcohol smell, soaked in 3% hydrochloric acid for 1d, Soak in 3% NaOH solution for 1d, and...
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