7-Phenylacetamido-3-desacetoxycephalosporanate and its preparation method and application

A technology of acetoxy cephalosporanic acid and ammonium salt of cyclo-expanded acid is applied in the field of pharmaceutical synthesis, and can solve the problems such as the influence of raw material drug quality, poor quality of 7-ADCA, etc., and achieve high product yield, short process time, and prolonged use. effect of life

Active Publication Date: 2022-05-03
方长明
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the quality of 7-ADCA synthesized by existing methods is poor, and its content is about 98wt%.
In addition, impurities such as isomers are generated during the preparation of 7-ADCA, especially the residual amount of the impurity Δ2-7-ADCA shown in the following structural formula (VIII) is very high, reaching 0.5 to 1.0%. The quality of the raw drug also has a big impact

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 7-Phenylacetamido-3-desacetoxycephalosporanate and its preparation method and application
  • 7-Phenylacetamido-3-desacetoxycephalosporanate and its preparation method and application
  • 7-Phenylacetamido-3-desacetoxycephalosporanate and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0066] The preferred synthetic route for preparing high-content 7-ADCA of the present invention is as follows:

[0067]

[0068] In order to obtain the 7-ADCA of high content, the present invention takes preferred technical scheme and comprises the steps:

[0069] (1) Preparation of high-content 7-phenylacetamido-3-desacetoxycephalosporanic acid and salts thereof.

[0070] The crude product of 7-phenylacetamido-3-desacetoxycephalosporanic acid is salt-formed and crystallized to obtain high-purity 7-phenylacetamido-3-desacetoxycephalosporanic acid salt; Add 7-phenylacetamido-3-desacetoxycephalosporanic acid salt into water, adjust the pH to 1-3 with acid, crystallize, and obtain high content of 7-phenylacetamido-3-desacetoxycephalosporanic acid .

[0071] (2) Preparation of high content 7-ADCA

[0072] The high-purity 7-phenylacetamido-3-desacetoxycephalosporanic acid or its salt obtained in the above step (1) is catalyzed by penicillin acylase to obtain 7-ADCA lysate, an...

Embodiment 1

[0111] Add 20g of crude product of cyclohexanoic acid (content 95.3wt%) into a 500ml reaction bottle, add 40ml of deionized water, add 50ml of acetone (for dispersion, can reduce the amount of water), stir, and dropwise add about 4.2ml of 24wt% ammonia water , adjust the pH to 6.5-7.0, stir for 30 min, add dropwise 400 ml of acetone, cool down to 0-5°C, and stir for 30 min. Filter, wash with 50 ml of acetone, filter dry, and dry under reduced pressure to obtain 19.53 g of cycloexpanded acid ammonium salt (HPLC purity 99.8%), yield 97.5% (mol / mol).

[0112] Add 10 g of the prepared ammonium cyclocyclate salt to 100 ml of water, adjust the pH to 6-8 to dissolve, add 5 g of immobilized penicillin acylase (immobilized enzyme), keep warm at 28-32°C, and maintain pH 7.5-8.5 with ammonia water , until the cleavage reaction is complete, the immobilized enzyme is filtered off to obtain the enzymatic hydrolysis solution. The temperature of the enzymolysis solution was raised to 30-35° ...

Embodiment 2

[0114] Add 20g of the crude product of cyclohexanoic acid (content 95.3wt%) into a 500ml reaction bottle, add 100ml of absolute ethanol, stir to dissolve, add about 4.2ml of 24wt% ammonia water dropwise, adjust the pH to 6.5-7.0, stir for 30min, and then add dropwise Add 200ml of ethanol, cool down to 0-5°C, and stir for 30min. Filter, wash with 50 ml of absolute ethanol, filter dry, and dry under reduced pressure to obtain 19.45 g of cycloexpanded ammonium salt (HPLC purity 99.8%). Yield 97.1% (mol / mol).

[0115] Add 10 g of the prepared cycloexpandant ammonium salt into 100 ml of water, adjust the pH to 6 to 8 to dissolve, add 5 g of immobilized penicillin acylase (immobilized enzyme), keep warm at 28 to 32 ° C, and maintain it with 4 wt % sodium hydroxide aqueous solution. pH 7.5-8.5, until the cleavage reaction is complete, filter out the immobilized enzyme, and obtain the enzymatic hydrolysis solution. The temperature of the enzymolysis solution was raised to 30-35° C.,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to 7-phenylacetamido-3-desacetoxy cephalosporanic acid salt and its preparation method and application. Described 7-phenylacetamido-3-desacetoxy cephalosporanate has the following structural formula: wherein, M + selected from metal ions or ammonium ions. A method for preparing 7-phenylacetamido-3-desacetoxycephalosporanic acid salt, comprising the steps of: reacting 7-phenylacetamido-3-desacetoxycephalosporanic acid with an alkaline salt-forming agent; Or it can be obtained by reacting 7-phenylacetamido-3-desacetoxy cephalosporanic acid trimethylsilyl ester with alkaline salt-forming agent. 7-ADCA can be prepared by 7-phenylacetamido-3-desacetoxycephalosporanate, and the content of the obtained 7-ADCA product is very high, especially the content of the isomer △2-7-ADCA Less than 0.5%, it is more suitable for synthesizing high-quality cephalexin bulk drugs such as cephalexin, cephradine, cefadroxil and ceftazidime pivoxil.

Description

technical field [0001] The invention belongs to the technical field of medicine synthesis, in particular to cephalosporin intermediate 7-phenylacetamido-3-desacetoxycephalosporanic acid salt, and 7-amino-3-desacetoxycephalosporanic acid (7- ADCA) preparation method. Background technique [0002] 7-amino-3-desacetoxy cephalosporanic acid (7-ADCA for short), its chemical structural formula is shown in following formula (I): [0003] [0004] 7-ADCA is an important mother nucleus for the synthesis of cephalosporin antibiotic raw materials, and a key intermediate for the synthesis of cephalexin, cephradine, cefadroxil and ceftazidime pivoxil. [0005] Regarding the synthetic method of 7-ADCA, the semi-synthetic chemical method is commonly used at present, that is, the cheap penicillin G potassium industrial salt with sufficient market supply is used as a raw material, and it is prepared through oxidation, ring expansion rearrangement, enzymatic cleavage, crystallization and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07D501/22C07D501/04C07D501/12C12P17/18
CPCC07D501/22C07D501/04C07D501/12C12P17/18
Inventor 方长明
Owner 方长明
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap