A method for identifying metabolites in honeysuckle
A technology of metabolites and identification methods, applied in the field of identification of metabolites in honeysuckle, can solve the problems of lack of identification methods for metabolites of honeysuckle and only focus on prototype components, etc., and achieve the effect of simple operation and good repeatability
Active Publication Date: 2022-02-11
SHANDONG ANALYSIS & TEST CENT
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Problems solved by technology
These studies show that many chemical components in honeysuckle are quickly metabolized into other compounds after entering the body. The inventors found that the existing research only focused on its prototype components, and lacked identification methods for the metabolites in honeysuckle.
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[0039] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
[0040] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.
[0041] A method for identifying metabolites in the body of honeysuckle, comprising the steps of:
[0042] 1) Ho...
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The invention discloses a method for identifying metabolites in the body of honeysuckle, which comprises the following steps: preparing the alcohol extract of honeysuckle, feeding the animals in the experimental group for several days, and feeding the control group with the same amount of normal saline; fasting after each feeding 8 hours, blood samples were taken, and the blood samples were analyzed by ultra-high performance liquid chromatography-high resolution mass spectrometry, and a peak table including mass number, retention time and peak intensity was obtained; the experimental group and the control group were given two factors of gavage days and honeysuckle Analysis of variance, screening out the variables only affected by honeysuckle, and then screening possible honeysuckle metabolites from the variables only affected by honeysuckle; performing secondary mass spectrometry analysis on possible honeysuckle metabolites, determined by neutral loss of 80 in secondary mass spectrometry It is a sulfated metabolite, and the neutral loss 176 is determined to be a glucuronidation metabolite; the neutral loss 80 and neutral loss 176 that appear simultaneously in the secondary mass spectrometry are the sulfated metabolites of glucuronide.
Description
technical field [0001] The patent of the present invention relates to the field of pharmaceutical analytical chemistry, and specifically relates to a method for identifying metabolites of honeysuckle in vivo. Background technique [0002] Traditional Chinese medicine has a history of thousands of years in our country and has made indelible contributions to the prosperity of the Chinese nation. Traditional Chinese medicine has a unique diagnosis and treatment method and a unique theoretical system. With social development and scientific and technological progress, medical care models and consumption concepts have gradually changed, and consumption trends of "returning to nature" and "advocating nature" are emerging around the world. Traditional Chinese medicine, as the primary representative of traditional medicine, has received widespread attention. Traditional Chinese medicine is safe, effective, has few toxic and side effects, and is famous for treating both symptoms and ...
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IPC IPC(8): G01N30/02G01N30/30G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/30G01N30/32G01N30/34G01N30/7266G01N2030/324
Inventor 李丽丽王晓刘伟马双双于金倩陈龙
Owner SHANDONG ANALYSIS & TEST CENT