Analysis method for simultaneously detecting polycyclic aromatic hydrocarbon and hydroxy metabolite level thereof in hair

A technology for polycyclic aromatic hydrocarbons and an analysis method is applied in the analysis field of simultaneous detection of polycyclic aromatic hydrocarbons and their hydroxy metabolites in hair, which can solve the problems of time-consuming and laborious, long method flow, inability to detect and analyze at the same time, etc. The effect of shortening time and avoiding errors

Inactive Publication Date: 2019-08-23
GUANGDONG UNIV OF TECH
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Problems solved by technology

[0004] At present, the literature has reported the analysis method of PAHs in hair, and a small number of studies have analyzed the pollution level of OH-PAHs in hair, and some studies have analyzed 52 kinds of OH-PAHs metabolites in the hair of rats fed poison, These existing analytical methods for OH-PAHs are all based on derivatized gas chromatography-mass spectrometry, which is long, time-consuming and labor-intensive.
On the other hand, because of the difference in physical and chemical properties of PAHs and OH-PAHs, it is impossible to use the same instrument conditions for simultaneous detection and analysis of them
That is to say, there is no suitable method for simultaneous detection of PAHs and OH-PAHs in human hair.

Method used

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  • Analysis method for simultaneously detecting polycyclic aromatic hydrocarbon and hydroxy metabolite level thereof in hair
  • Analysis method for simultaneously detecting polycyclic aromatic hydrocarbon and hydroxy metabolite level thereof in hair
  • Analysis method for simultaneously detecting polycyclic aromatic hydrocarbon and hydroxy metabolite level thereof in hair

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Embodiment 1

[0029] The hair samples in this example were collected from occupationally exposed dismantling workers in an electronic waste dismantling area and nearby non-occupationally exposed residents. All samples were tightly wrapped with aluminum foil, sealed in sealed bags and stored at -20°C.

[0030] 1. Take 1g of hair sample, add 20mL of acetone and ultrasonically clean it for 1 minute to obtain the exogenous extract; dry the washed hair, cut it to about 1mm, put it in a 50mL Teflon centrifuge tube, add the PAHs recovery indicator Nap- d 8 、Ace-d 10 , Phe-d 10 , Chr-d 12 , Pery-d 12 And OH-PAHs isotope internal standard 2-OH-Nap-d 7 , 2-OH-Flu-d 9 , 1-OH-Pyr-d 9 , 13 C 12 -3-OH-Phe, then add 5mL 1M NaOH to the dried hair, and digest it in a water bath at 40°C for 12 hours to obtain an alkaline digestion solution; then extract it with a mixed solution of n-hexane / tert-butyl methyl ether to obtain endogenous sex extract;

[0031] 2. After the extract was concentrated, it ...

Embodiment 2

[0038] The established method can be applied to the detection of exogenous and endogenous PAHs and OH-PAHs in the hair of occupationally exposed workers and non-exposed residents. Except for a few exogenous OH-PAHs, the detection rate of PAHs and OH-PAHs in exogenous hair was 100%. The average total concentration of the 16 exogenous PAHs in the hair of non-exposed residents was 116ng / g hair dry weight, and the endogenous concentration was 164ng / g; the average total concentration of the exogenous 16 PAHs in the hair of exposed workers was 111ng / g, and the endogenous concentration was 292ng / g; of which Phe, Fluo, Pyr and Chr are the main components. The distribution of these four PAHs is different in internal and external sources. The internal source is Phe27.5%>Pyr 20%>Fluo 19.5%>Chr 8.5%, and the exogenous source is Pyr 21.5%>Fluo 20.5%>Phe 15%>Chr 11.5%.

[0039] On the other hand, the average total concentration of OH-PAHs in exogenous hair of non-exposed residents was 10 ...

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Abstract

The invention discloses an analysis method for simultaneously detecting polycyclic aromatic hydrocarbon and hydroxy metabolite level thereof in hair. The method comprises the following steps: cleaningthe hair by using acetone to obtain exogenous extract liquor, digesting the cleaned hair under the condition of 40-80 DEG C to obtain endogenous extract liquor; concentrating the exogenous extract liquor and the endogenous extract liquor, performing purification by using gel permeation chromatographic column, eluting by using eluant, and collecting leacheate; performing separation and purification after concentrating the leacheate; eluting by using a n-hexane solution containing 2-5% of polar solvent, and collecting the component 1, and then eluting by using the n-hexane solution containing 30-60% of polar solvent, and collecting the component 2, performing nitrogen blowing and metered volume on the eluant, and obtaining the content level of a target object in the hair through data processing and quantitative calculation. Multiple exogenous and endogenous polycyclic aromatic hydrocarbons and the hydroxy metabolites thereof in the hair can be detected at the same time, thereby providing an effective method means for the human body exposure health risk evaluation of the polycyclic aromatic hydrocarbons.

Description

technical field [0001] The invention belongs to the technical field of organic pollutant analysis, and more particularly relates to an analysis method for simultaneously detecting polycyclic aromatic hydrocarbons (PAHs) and hydroxyl metabolites (OH-PAHs) in hair. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of compounds formed by the fusion of two or more aromatic rings, which are commonly found in the natural environment. PAHs mainly come from the incomplete combustion of hydrocarbons. So far, more than 200 PAHs homologues have been discovered, most of which are persistent and strong carcinogenic, teratogenic, and mutagenic, and have relatively strong effects on the ecological environment and human health. big threat. Among them, 16 kinds of PAHs are listed as priority pollutants by the US Environmental Protection Agency, and 7 kinds of PAHs are also included in the priority monitoring list in my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/062
Inventor 马盛韬林美卿余应新安太成
Owner GUANGDONG UNIV OF TECH
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