Wood vinegar microbial livestock and poultry feed additive and preparation method and application thereof
A technology for additive preparation, livestock and poultry feed, applied in the direction of animal feed, animal feed, application, etc., can solve the problem of not getting due promotion and application, and achieve the goal of promoting the growth of livestock and poultry, enhancing disease resistance, and improving ADG. Effect
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[0027] The present invention will be described in detail below in conjunction with examples.
[0028] The percentage by mass of the raw materials of the additive in the present invention is: 99.93%-99.75% of the Bacillus subtilis liquid, 0.07%-0.25% of the refined wood vinegar semen;
[0029] Prepare LB basal medium: use 1mol / L HCL solution and NaOH solution to control the pH value between 6.9‾7.1, autoclave at 121°C for 15min, and cool to room temperature;
[0030] Preparation of bacillus bacteria liquid: Inoculate activated bacillus into the above-mentioned LB basal medium at 2% (mass percentage), seal the bottle mouth, and incubate at 37°C, 180r / min incubator with constant temperature and shaking for 23‾25h;
[0031] Preparation of Bacillus bacteria liquid in wood vinegar: add refined wood vinegar at 0.05%-0.2% (mass percentage) to the above-mentioned Bacillus bacteria liquid, and incubate in a shaking incubator at 37°C and 180r / min for 7-9 hours; the obtained The yellow-b...
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