Photosynthetic bacterium Rhodobacter sphaeroides strain, bacterial agent, preparation method and application thereof
A technology of Rhodobacter sphaeroides and photosynthetic bacteria, applied in the fields of botany equipment and methods, biochemical equipment and methods, bacteria, etc., can solve the problems of difficulty and high cost in the control of rice bacterial streak disease
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[0036] According to another aspect of the present invention, also provide a kind of preparation method of the biocontrol agent of photosynthetic bacterium Rhodobacter sphaeroides strain as above-mentioned, comprise the following steps: Cultivate by multi-layer plate culture method until red single colony appears; S2, seed culture: insert the red single colony cultivated in step S1 into a serum bottle, and carry out seed culture with photosynthetic bacteria liquid medium to the logarithmic growth phase to obtain Bacteria liquid; S3, production cultivation: inoculate the bacterium liquid in the step S2 by the inoculation amount of 3%~6% of the total volume of the photosynthetic bacteria strain production medium, cultivate to the logarithmic growth phase to obtain the biocontrol of the photosynthetic bacteria Rhodobacter sphaeroides strain Bacteria.
[0037] In this embodiment, the temperature for seed cultivation in step S2 is 30°C to 35°C, the light conditions are 2500Lx to 400...
Embodiment 1
[0054] The inhibitory effect of the photosynthetic bacteria strain Rhodobacter sphaeroides A13 on the pathogenic bacteria causing rice bacterial stripe disease was measured by the inhibition zone method. Take 0.1ml of pathogenic bacteria and smear it on the LB plate, put a sterilizing steel ring in the center, and Add 0.05ml of photosynthetic bacteria solution to the steel ring, incubate at 30°C for 7 days, and observe whether there is a zone of inhibition.
Embodiment 2
[0056] Under greenhouse conditions, the 5×10 6 cfu / mL photosynthetic bacterium Rhodobacter sphaeroides A13 strain biocontrol fungus Soak the rice seeds germinated to white at 30°C for 1 hour, sow the treated rice seeds in pots, and keep the seedlings at 28°C during the identification period. Cultivate in a greenhouse under 10h / d light conditions, and record the plant height regularly. Three representative plants were selected to measure the plant height and wet weight respectively. After the rice enters the seedling stage, the rice seedlings are inoculated with Xanthomonas oryzae, and the disease index is investigated 7 days after the inoculation and the control effect is calculated.
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