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Two-line hybridization method for producing sorghum seeds

A production method and sorghum technology, which are applied to the field of two-line hybrid sorghum seed production, can solve problems such as low starting temperature, genetic drift of starting temperature, and increased proportion, and achieve the effects of ensuring purity, overcoming genetic drift, and improving safety factor.

Inactive Publication Date: 2003-03-05
湖南省农业科学院高粱研究开发中心
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AI Technical Summary

Problems solved by technology

However, in the process of using Xiangnuoliang S-1 to produce two-line sorghum hybrid seeds, there are still two problems in the following aspects: 1. Due to the low starting temperature for inducing the stable sterility of dual-purpose sterile lines, it makes safe seed production The selection range of the ecological point is narrow, which is only suitable for winter seed production in Hainan, and it is difficult to produce large-scale seed; 2. Due to the phenomenon of genetic drift in the starting temperature of the dual-use male sterile line's fertility conversion, under the temperature and light conditions of seed production, the two The proportion of fertile plants using sterile lines rises to more than 50%, and the purity of hybrid seeds produced is difficult to guarantee

Method used

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Experimental program
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Effect test

Embodiment Construction

[0007] 1. Xiangbai waxy sorghum was crossed with TX3197B, and then transgenic with TX3197A. Among the segregated offspring, the white-grained waxy sorghum with temperature- and light-sensitive fertility characteristics was selected as the breeding material, and sowed in mid-April and late, respectively. Heading and flowering were carried out under high temperature and selective pressure in the first ten days and middle ten days. Observe the fertility performance, pull out the fertile and semi-fertile plants, and leave the sterile plants to cut the stalks for regeneration. The regenerated sorghum was headed and flowered under high temperature conditions in the middle and late August, showing fertility and self-fertilization. After multiple generations of stable breeding, two dual-purpose CMS lines of waxy sorghum with high starting temperature, Xiangnuoliang 2S (24.5℃) and Xiangnuoliang 3S (26.5℃) were bred. Use Xiangnuoliang 2S single ear as material, sow in early April, and ...

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Abstract

A two-line hybridization method for breeding sorghum features that cultivating the dual-purpose sterile line having high origin temp from the sorghum seeds whose fertility has temp and light sensitivity by selecting layering object at 24.5-26.5 deg.C, obtaining purified and stable dual-purpose sterile line by creating the purifying and reproducing system of key seeds by controlling temp and light radiation in the sensitive period of fertility transform, and cultivating two-line hybridized seeds at proper ecological condition. Its advantages are wide range of production, high safety and high purity.

Description

technical field [0001] The invention relates to the production of sorghum seeds, in particular to a production method of two-line hybrid sorghum seeds. Background technique [0002] The use of heterosis in sorghum, since the first cytoplasmic male sterile line TX3197A was bred in the United States in the 1950s, the three-line method is mainly used in the world. The utilization of sorghum heterosis in the two-line method is based on the three-line method, omitting the maintainer line, and relying on the temperature- and light-sensitive characteristics of the dual-purpose sterile line of sorghum, that is, it is fertile under certain temperature and light conditions. It is used for self-propagation and reserved seeds; it is sterile under another temperature and light condition, and is used for hybridization with restorer lines to produce first-generation hybrid seeds. In 1993, India reported the discovery of sorghum photothermosensitive cytoplasmic male sterile material, and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 刘海军汤文光尹颛斌曾贤杰叶桃林杨光立李光林刘登望
Owner 湖南省农业科学院高粱研究开发中心
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