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A method for screening linear spirulina filaments by using triclosan

A triclosan, linear technology, applied in the field of spirulina production, can solve the problems of low success rate, long time, complicated procedures, etc., and achieve the effect of low cost and high-throughput screening

Active Publication Date: 2021-02-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is still used at home and abroad up to now, "picking linear algal filaments under a microscope → cultivating algal filaments for about 1 month → microscopic detection → if there are spiral algal filaments, then picking linear algal filaments for culture →…→Until the linear algae filament population is obtained”, this traditional method of screening linear Spirulina algae filaments has cumbersome procedures, long time-consuming, and low success rate

Method used

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Experimental program
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Embodiment Construction

[0021] The technical solutions of the present invention will be described in detail below in conjunction with specific implementation examples.

[0022] 1. Selection of sample materials: 9 well-known strains of Spirulina platensis CHM-5, CHM-6, CHM-7, CHM-8, CHM-9, CHM-10 widely used in scientific research and industrial production , CHM-11, ZJU0112, and ZJU0137 are also preserved in the Institute of Nuclear Agricultural Sciences of Zhejiang University affiliated to the applicant. The applicant guarantees that samples can be provided to the public as needed during the validity period of the patent.

[0023] 2. Reagents and instruments: Triclosan used in the present invention is the product of Jiangsu Yike Chemical Co., Ltd.; other reagents are analytically pure; the optical density T of the algae liquid is measured with an Ultrospec 2000 UV-Vis spectrophotometer from Pharmacia, Sweden.

[0024] 3. The method for screening linear spirulina filaments is as follows:

[0025] (1...

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PUM

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Abstract

The invention relates to the production technology of spirulina and aims to provide a method for screening linear spirulina filaments by using triclosan. Including: take an appropriate amount of spirulina algae liquid with both spiral and linear algal filaments, dilute its turbidity T to 0.2 with Zarrouk's culture medium; divide the diluted algae liquid into multiple parts, add Dilute the turbidity T of the algae solution to 0.1 in the Zarrouk culture solution containing gradient concentrations of triclosan; after 70 days of standing culture, select the blue-green algae solution, and take the group with the highest concentration of triclosan, and the other group The algae filaments in the algae liquid are all linear algae filaments. The present invention screens linear algae filaments based on the difference in susceptibility of different algae filaments to triclosan. Compared with the traditional separation and cultivation method of algae filaments, the present invention is not only simple, efficient, but also low in cost, and can realize batch, automation, and high-throughput Quantity screening. The method can provide the required linear spirulina filaments for further research on the internal mechanism and influencing factors of the straightening of the spirulina filaments.

Description

technical field [0001] The invention belongs to the production technology of spirulina, in particular to a method for screening linear spirulina filaments by using triclosan. Background technique [0002] Spirulina (Spirulina) is a genus of Cyanophyta, Oscillatoriaceae, and Oscillatoriaceae, with at least 38 species, including A. platensis and A. maxima, etc. A small number of species have achieved large-scale industrial farming and are widely used in many industries such as food, feed, aquatic bait, medicine and health care, and fine chemical industry due to their characteristics of food safety, high nutritional health care and medical value, and good production performance. Economic microalgae with the largest scale and the widest application fields in scientific research and industrialization development at home and abroad. [0003] The typical morphological characteristics of Spirulina filaments in taxonomy are multiple columnar cells connected in series into filamentou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 汪志平余超李晨晨卢奇奇
Owner ZHEJIANG UNIV
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